Impact and Modulations of Peripheral and Edaphic B Cell Subpopulations in Chronic Rhinosinusitis With Nasal Polyposis
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Objectives. The pathophysiological mechanisms of chronic rhinosinusitis with nasal polyposis (CRSwNP) still are discussed controversially. Regulatory B cells (Breg) are responsible for the suppression of T cell activity: deficiencies for Breg have been demonstrated to contribute to autoimmune disorders, e.g., systemic lupus erythematosus. In order to evaluate the influence of B cell subpopulations, especially Breg, on the etiology of this disease, the aim of this study was to characterize subpopulations of peripheral and edaphic B cells in CRSwNP. Methods. Polypoid tissue and blood samples were collected from 10 patients undergoing paranasal sinus surgery and lymphocytes were analyzed by multicolor flow cytometry. Results. There was a significantly lower frequency of B cells in nasal polyps compared to peripheral blood mononuclear cells (PBMC) in patients with CRSwNP. Mature resting B cells were the main population within B cells in PBMC, and memory B cells in nasal polyps. Remarkably, Breg and mature B cells significantly decreased in nasal polyps compared to PBMC. Memory B cells significantly increased and represented the main subpopulation in nasal polyps in patients with CRSwNP. Conclusion. In this study a detailed contemporary characterization of B cell subpopulations in patients with CRSwNP is presented. The influence of edaphic B cells could play a key role in the maintenance of this chronic infectious disease.
Abstract
Objectives. The pathophysiological mechanisms of chronic rhinosinusitis with nasal polyposis (CRSwNP) still are discussed controversially. Regulatory B cells (Breg) are responsible for the suppression of T cell activity: deficiencies for Breg have been demonstrated to contribute to autoimmune disorders, e.g., systemic lupus erythematosus. In order to evaluate the influence of B cell subpopulations, especially Breg, on the etiology of this disease, the aim of this study was to characterize subpopulations of peripheral and edaphic B cells in CRSwNP. Methods. Polypoid tissue and blood samples were collected from 10 patients undergoing paranasal sinus surgery and lymphocytes were analyzed by multicolor flow cytometry. Results. There was a significantly lower frequency of B cells in nasal polyps compared to peripheral blood mononuclear cells (PBMC) in patients with CRSwNP. Mature resting B cells were the main population within B cells in PBMC, and memory B cells in nasal polyps. Remarkably, Breg and mature B cells significantly decreased in nasal polyps compared to PBMC. Memory B cells significantly increased and represented the main subpopulation in nasal polyps in patients with CRSwNP. Conclusion. In this study a detailed contemporary characterization of B cell subpopulations in patients with CRSwNP is presented. The influence of edaphic B cells could play a key role in the maintenance of this chronic infectious disease.
참고문헌 (30)
- Larsen PL, "Origin of nasal polyps", Laryngoscope, 101(3), pp.305~312 (1991)
- Andrews AE, "Site of origin of nasal polyps: relevance to pathogenesis and management", Rhinology, 43(3), pp.180~184 (2005)
- Bachert C, "Current and future treatment options for adult chronic rhinosinusitis: focus on nasal polyposis", J Allergy Clin Immunol, 136(6), pp.1431~1440 (2015)[DOI]
- Ickrath P, "Characterization of T-cell subpopulations in patients with chronic rhinosinusitis with nasal polyposis", Allergy Rhinol (Providence), 8(3), pp.139~147 (2017)
- Dutsch-Wicherek M, "The analysis of RCAS1 and DFF-45 expression in nasal polyps with respect to immune cells infiltration", BMC Immunol, 7, pp.4~ (2006)[DOI]
- Zhang LP, "T-lymphocyte subpopulations and B7-H1", PD-1 expression in nasal polyposis, 2010, pp.38(2), 593~601
- Dutsch-Wicherek M, "The presence of B7-H4+ macrophages and CD25+CD4+ and FOXP3+ regulatory T cells in the microenvironment of nasal polyps: a preliminary report", Folia Histochem Cytobiol, 48(4), pp.611~617 (2010)
- Gevaert P, "Local receptor revision and class switching to IgE in chronic rhinosinusitis with nasal polyps", Allergy, 68(1), pp.55~63 (2013)[DOI]
- Van Zele T, "Local immunoglobulin production in nasal polyposis is modulated by superantigens", Clin Exp Allergy, 37(12), pp.1840~1847 (2007)[DOI]
- Bachert C, "Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma", J Allergy Clin Immunol, 126(5), pp.962~968 (2010)[DOI]
- Hoyer BF, "Long-lived plasma cells and their contribution to autoimmunity", Ann N Y Acad Sci, 1050, pp.124~133 (2005)[DOI]
- Blair PA, "CD19(+)CD24(hi)CD38(hi) B cells exhibit regulatory capacity in healthy individuals but are functionally impaired in systemic lupus erythematosus patients", Immunity, 32(1), pp.129~140 (2010)[DOI]
- Odendahl M, "Generation of migratory antigen-specific plasma blasts and mobilization of resident plasma cells in a secondary immune response", Blood, 105(4), pp.1614~1621 (2005)[DOI]
- Jacobi AM, "Correlation between circulating CD27high plasma cells and disease activity in patients with systemic lupus erythematosus", Arthritis Rheum, 48(5), pp.1332~1342 (2003)[DOI]
- Robinson DS, "The role of regulatory T lymphocytes in asthma pathogenesis", Curr Allergy Asthma Rep, 5(2), pp.136~141 (2005)[DOI]
- Miyara M, "Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor", Immunity, 30(6), pp.899~911 (2009)[DOI]
- Fokkens WJ, "EPOS 2012: European position paper on rhinosinusitis and nasal polyps 2012. A summary for otorhinolaryngologists", Rhinology, 50(1), pp.1~12 (2012)
- Derycke L, "Mixed T helper cell signatures in chronic rhinosinusitis with and without polyps", PLoS One, 9(6), pp.e97581~ (2014)[DOI]
- Kato A, "Airway epithelial cells produce B cell-activating factor of TNF family by an IFN-beta-dependent mechanism", J Immunol, 177(10), pp.7164~7172 (2006)[DOI]
- Kato A, "Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps", J Allergy Clin Immunol, 121(6), pp.1385~1392 (2008)[DOI]
- Patadia M, "Evaluation of the presence of B-cell attractant chemokines in chronic rhinosinusitis", Am J Rhinol Allergy, 24(1), pp.11~16 (2010)[DOI]
- Peters AT, "Evidence for altered activity of the IL-6 pathway in chronic rhinosinusitis with nasal polyps", J Allergy Clin Immunol, 125(2), pp.397~403 (2010)[DOI]
- Weksler ME, "Changes in the B-cell repertoire with age", Vaccine, 18(16), pp.1624~1628 (2000)[DOI]
- Agematsu K, "B cell subpopulations separated by CD27 and crucial collaboration of CD27+ B cells and helper T cells in immunoglobulin production", Eur J Immunol, 27(8), pp.2073~2079 (1997)[DOI]
- Belkaid Y, "Regulatory T cells in the control of host-microorganism interactions (*)", Annu Rev Immunol, 27, pp.551~589 (2009)[DOI]
- Tan BK, "Evidence for intranasal antinuclear autoantibodies in patients with chronic rhinosinusitis with nasal polyps", J Allergy Clin Immunol, 128(6), pp.1198~1206 (2011)[DOI]
- Gevaert P, "Omalizumab is effective in allergic and nonallergic patients with nasal polyps and asthma", J Allergy Clin Immunol, 131(1), pp.110~116 (2013)[DOI]
- Dorner T, "B-cell-directed therapies for autoimmune disease", Nat Rev Rheumatol, 5(8), pp.433~441 (2009)[DOI]
- Murray T, "Very late antigen-1 marks functional tumor-resident CD8 T cells and correlates with survival of melanoma patients", Front Immunol, 7, pp.573~ (2016)
- Rau M, "Progression from nonalcoholic fatty liver to nonalcoholic steatohepatitis is marked by a higher frequency of TH17 cells in the liver and an increased TH17", resting regulatory T cell ratio in peripheral blood and in the liver, 2016, pp.196(1), 97~105