국내 자생 식물추출물의 DPPH radical 소거활성 및 항균성 스크리닝
Screening of DPPH radical scavenging and antimicrobial activity of extracts from local some native plants
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초록
항산화 및 항균활성을 가진 천연물을 활용해 합성보존료를 대체하고자 하는 연구가 활발한 가운데 국내 자생 식물 중 135종을 대상으로 DPPH radical 소거활성을 측정하였다. 이중 항산화 활성이 10%대로 낮게 나타난 식물 8종에 대해 항균효과를 조사하였다. 식물 추출물의 DPPH radical 소거활성은 0.41～94.84%로 다양하였으며, 가장 효과가 좋은 식물은 꽃향유와 우엉으로 각각 94.84, 80.55%를 나타내었다. 우엉은 잎의 경우 26.87, 뿌리 19.29%의 활성을 나타내 동일 식물이라도 추출부위에 따라 활성의 차이가 있을 것으로 보인다. 선이질풀과 비쭈기나무는 각각 그람양성균인 B. subtilis, 그람음성균인 E. coli 및 Candida속인 C. albicans에 대해 2.2～10.8 mm 수준의 항균활성을 나타내었다. 각 식물에 대한 다양한 추출법을 적용하여 기능성을 확인하고 이들 항산화성 물질에 대한 추가 연구가 진행되어야 할 것으로 사료된다.
Abstract
This study was conducted to determine the antioxidant potentials of some available plants to source alternate antioxidants and antibiotics. The antioxidant activity was evaluated by determining the antioxidant activity reducing powers and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities of extracts from some local edible native plants. The DPPH radical scavenging activity of the extracts was found to have been 0.41-94.84%. The Elsholtziasplendens NAKAI extract (85ºC, ethanol, accelerated solvent extraction) showed the highest level of antioxidant activity. Eight samples of plant extracts were evaluated for their antimicrobial activities against three microflora (Bacillus subtilis, Escherichia coli, and Candida albicans) using disc diffusion assay. Two samples (Geranium krameri FR. Et SAV. and Cleyera japonica Thunb.) had much more intensive antimicrobial activities than the control. However, their DPPH free radical scavenging activity levels were only 14.50 and 13.85, respectively. It is suggested that they could be used as natural preservatives against bacterial contamination in cosmetics and foods, in place of the common synthetic preservatives currently used.
참고문헌 (18)
- Gupta VK, "Plants as natural antioxidants", Nat Prod Radiance, 5, pp.326~334 (2006)
- MS Brewer, "Natural antioxidants: sources, compounds, mechanisms of action, and potential applications", Compr Rev Food Sci Food Safety, 10, pp.221~247 (2011)
- 사여진, "국내산 수수(Sorghum bicolor) 품종에 따른 전자공여능, 환원력, 항균활성 및 α-glucosidase 저해활성의 비교", 한국식품과학회지, 42(5), pp.598~604 (2010), 한국식품과학회
- Ifesan BOT, "Antioxidant and antimicrobial properties of selected plant leaves", Eur J Med Plants, 3, pp.465~473 (2013)
- Nik AK, "In vitro antimicrobial and antioxidant activity of commercial rosemary extract formulations", J Food Prot, 72, pp.174~1752 (2009)
- Fattouch S, "Comparative analysis of polyphenolic profiles and antioxidant and antimicrobial activities of tunisian pome fruit pulp and peel aqueous acetone extracts", J Agric Food Chem, 56, pp.1084~1090 (2008)
- Singh G, "A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents", Food Chem Toxicol, 45, pp.1650~1661 (2007)
- 허문수, "제주도 자생식물 메탄올 추출액의 항산화 및 항균효능 검색", KSBB Journal, 22(2), pp.78~83 (2007), 한국생물공학회
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- 이영민, "천연 식물자원에서 DPPH라디칼 제거능과 Proly Endopeptidase 활성 저해능 탐색", 한국지역사회생활과학회지, 16(4), pp.95~102 (2005), 한국지역사회생활과학회
- Sharma OP, "DPPH antioxidant assay revisited", Food Chem, 113, pp.1201~1205 (2009)
- RS Farag, "Antimicrobial activity of some Egyptian spice essential oils", J Food Prot, 52, pp.665~670 (1989)
- Musialik M, "Scavenging of DPPH radicals by vitamin E is accelerated by its partial ionization: the role of sequential proton loss electron transfer", Org Lett, 7, pp.4951~4954 (2005)
- Lee JA, "Screening of the antioxidant and anti-elastase activities for the extracts of Jeju endemic plants", Res Sci Educ, 13, pp.249~269 (2011)
- Woo WS, "Biological evaluation of Korean medical plants(III)", Arch Pharm Res, 2, pp.127~188 (1979)
- Lee BH, "Composition for preventing hair loss or promoting hair growth comprising extract of Geranium sibiricum L", ~ (2014)
- Lee MW, "Novel hydrolysable tannis isolated from the leaves of Cleyera japonica Thunberg and anti-oxidative use thereof", ~ (2014)
- 오순자, "제주도 자생 차나무과 식물의 ACE, APN, α-amylase 저해 활성 및 항산화활성에 대한 연구", 한국자원식물학회지, 23(5), pp.406~414 (2010), 한국자원식물학회