이팝나무 꽃에서 분리한 페놀 화합물의 생리활성 효소 억제효과
Inhibitory activities of phenolic compounds isolated from Chionanthus retusa flower on biological enzymes
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
This study was designed to determine the biological activities of Chionanthus retusus flower extracts. Water and 90% ethanol extracts of C. retusus flower were prepared. The inhibitory activities of water and ethanol extracts with a phenolic content of 200 µg/mL against xanthine oxidase were 25.60% and 15.92%, respectively. Further, the water extract did not show any inhibitory activity against α-glucosidase whereas the ethanol extract showed 100.00% inhibition of α-glucosidase. The inhibitory activities of the extracts against tyrosinase were 17.27% (water extract) and 36.13% (ethanol extract), which suggest that the extracts may have a whitening effect. The water extract did not inhibit elastase activity but showed a collagenase-inhibitory activity of 20.21%. On the contrary, the ethanol extract showed 96.26% and 35.93% inhibition of collagenase and elastase, respectively. These findings suggest that the extracts may have an anti-wrinkle effect. Lastly, the extracts showed a hyaluronidase inhibitory activity of 36.96% (water extract) and 88.70% (ethanol extract), suggesting that they may have an anti-inflammatory effect. The results indicate that C. retusus flower extracts containing phenolic compounds can be used as functional resources because they have anti-gout, carbohydrate degradation-inhibitory, whitening, anti-wrinkle, and anti-inflammatory effects.
Abstract
This study was designed to determine the biological activities of Chionanthus retusus flower extracts. Water and 90% ethanol extracts of C. retusus flower were prepared. The inhibitory activities of water and ethanol extracts with a phenolic content of 200 µg/mL against xanthine oxidase were 25.60% and 15.92%, respectively. Further, the water extract did not show any inhibitory activity against α-glucosidase whereas the ethanol extract showed 100.00% inhibition of α-glucosidase. The inhibitory activities of the extracts against tyrosinase were 17.27% (water extract) and 36.13% (ethanol extract), which suggest that the extracts may have a whitening effect. The water extract did not inhibit elastase activity but showed a collagenase-inhibitory activity of 20.21%. On the contrary, the ethanol extract showed 96.26% and 35.93% inhibition of collagenase and elastase, respectively. These findings suggest that the extracts may have an anti-wrinkle effect. Lastly, the extracts showed a hyaluronidase inhibitory activity of 36.96% (water extract) and 88.70% (ethanol extract), suggesting that they may have an anti-inflammatory effect. The results indicate that C. retusus flower extracts containing phenolic compounds can be used as functional resources because they have anti-gout, carbohydrate degradation-inhibitory, whitening, anti-wrinkle, and anti-inflammatory effects.
참고문헌 (34)
- Harman DA, "Theory based on free radical and radiation chemistry", J Gerontol, 11, pp.298~300 (1956)
- 조영제, "미세분쇄에 의한 감국(Chrysanthemum incidicum Linne) 추출물의 생리활성", 한국식품영양과학회지, 43(1), pp.110~117 (2014), 한국식품영양과학회
- Duke EJ, "Characterization of altenative molecular forms of xanthine oxidase in the mouse", Biochem J, 131, pp.187~190 (1973)
- 김정환, "진범(Aconitum pesudo-laeve var. erectum) 추출물의 항노화 및 항당뇨 효과", 생명과학회지, 23(5), pp.616~621 (2013), 한국생명과학회[DOI]
- Kameyama K, "Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1 (TRP 1), dopachrome tatumerase (TRP 2) and a melanogenic inhibitor", J Invest Dermatol, 100, pp.126~131 (1993)
- Grant NH, "Studies on the collagenase of Clostridium histolyticum", Arch Biochem Biophys, 82, pp.245~255 (1959)
- Kligman D, "Cosmeveuticals", Dermatol Clin, 18, pp.609~615 (2000)
- Jeong SJ, "Hyaluronidase inhibitor from uncariae ramulus et uncus", Korean J Pharmacogn, 29, pp.169~172 (1998)
- 채정우, "정금나무(Vaccinium oldhami) 열매의 생리활성 및 항균활성", 한국식품영양과학회지, 41(1), pp.1~6 (2012), 한국식품영양과학회
- Kim JW, "Physicochemical and physiological properties of Aster scaber Thunb., and utilization as food materials", pp.2014
- 유영춘, "RAW 264.7 대식세포에 미치는 병꽃나무 꽃 추출물의 항산화 및 항염증 효과", 생명과학회지, 26(3), pp.338~345 (2016), 한국생명과학회
- 임명희, "백련꽃 추출물의 생리활성 효과", 한국지역사회생활과학회지, 19(1), pp.3~10 (2008), 한국지역사회생활과학회
- 손형우, "도화 및 도화악 추출물의 미백활성 비교", Food Science and Preservation, 19(6), pp.946~950 (2012), 한국식품저장유통학회
- Lee TB, "Illustrated flora of korea", pp.616~ (1999), Hyangmunsa Publishing Co
- Kim IK, "Studies on seed germination of fringe tree (Chionathus retusus)", Korean J Hort Sci Technol, 6, pp.212~213 (1988)
- Chien CT, "Storage behavior of Chionanthus retusus seed and asynchronous development of the radicle and shoot apex during germination in relation to germination inhibitors, including abscisic acid and four phenolic glucosides", Plant Cell Physiol, 45, pp.1158~1167 (2004)
- Harborne JB, "A chemotaxonomic survey of flavonoids in leaves of the Oleaceae", Bot J Linn Soc, 81, pp.155~167 (1980)
- Kang MW, "Phytochemical constituents and biological activity of Chionanthus retusus Lindl. et Paxton", pp.2005
- Kim MS, "Anti-oxidative activities of extracts from Chionanthus retusus leaves, fruits and flower", Curr Res Agric Life Sci, 33, pp.49~56 (2015)
- Stirpe F, "The regluation of rat liver xanthine oxidase", J Biol Chem, 244, pp.3855~3863 (1969)
- Tibbot BK, "Molecular cloning and characterization of a gibberellin-indncible, putative <TEX>$\alpha$<", TEX> -glucosidase gene from barley, 1996, pp.30, 229~241
- Vincent J, "Mammalian monophenol monooxygenase (tyrosinase): Purification, properties, and reactions catalyzed", Method Enzymol, 142, pp.154~165 (1987)
- Wunsch E, "Zur quantitativen bestimmung der kollagenase", Hoppe-Seyler's Physiol Chem, 333, pp.149~151 (1963)
- Kraunsoe JAE, "Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor", Biochemistry, 35, pp.9090~9096 (1996)
- Dorfman A, "A turbidimetric method for the assay of hyaluronidase", J biol chemi, 172, pp.367~375 (1948)
- Mo SM, "Diet therapy", pp.311~ (1986), Kyomunsa Publishing Co
- 김경범, "유근피(Ulmus pumila)로부터 분리한 phenol성 물질의 건강기능식품 활성", Food Science and Preservation, 19(6), pp.909~918 (2012), 한국식품저장유통학회
- Choi HJ, "Inhibitory effect of Gyrophora esculenta on <TEX>$\alpha$<", TEX>-glucosidase, Kyunghee University, 2000
- 모정희, "측백나무 추출물의 Tyrosinase 활성저해 및 Melanin 생성 저해", 아시안뷰티화장품학술지, 13(2), pp.189~194 (2015), 한국피부과학연구원
- Giacomoni PU, "Factors of skin ageing share common mechanisms", Biogerontology, 2, pp.219~229 (2001)
- 안병권, "추출용매에 따른 함초 추출물의 피부미용 효과", 공업화학, 22(1), pp.56~60 (2011), 한국공업화학회
- 이병근, "화장품 개발을 위한 배롱나무(Lagerstroemia indica Linnaeus) 가지 추출물의 생리활성에 관한 연구", 한국자원식물학회지, 27(1), pp.29~34 (2014), 한국자원식물학회[DOI]
- 김경범, "유근피(Ulmus pumila)로부터 분리한 페놀성물질의 미용식품활성", Journal of Applied Biological Chemistry, 55(4), pp.207~215 (2012), 한국응용생명화학회
- Srern R, "Devising a pathway for hyaluronan catabolism: are we there yet?", Glycobiology, 13, pp.105~115 (2003)