LPS 자극 RAW 264.7 세포에서 제주산 올리브 추출물의 항염 활성
Anti-inflammatory activities of Olea europaea extracts from Jeju Island on LPS-induced RAW 264.7 cells
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Lipopolysaccharide (LPS)-induced activation of murine RAW 264.7 cells results in the production of pro-inflammatory cytokines including PGE2, IL-6, TNF-α, and IL-1β. In this study, we investigated the anti-inflammatory effects of the Olea europaea extract from Jeju island using LPS which induces an inflammatory response. To examine the potential anti-inflammatory properties of the Olea europaea extract, we measured the amount of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and IL-1β on RAW 264.7 cells after the Olea europaea extract treatment. The Olea europaea extracts showed higher anti-inflammatory activity by inhibiting the production of PGE2, NO, TNF-α, IL-6, and IL-1β induced by LPS stimulation in RAW 264.7 cells. Also, Olea europaea extracts decreased mRNA expression of iNOS, and COX-2. There was no cytotoxicity in the macrophage proliferation treated with the Olea europaea extract compared to the control in the LDH assay. These results suggest that the Olea europaea extract may exert significant effects on inflammatory factors and be a potential source as a new natural anti-inflammatory agent. Further investigations will focus on cell-based in vitro assays and in chemically identifying the major active components mediating the anti-aging and anti-inflammation responses.
Abstract
Lipopolysaccharide (LPS)-induced activation of murine RAW 264.7 cells results in the production of pro-inflammatory cytokines including PGE2, IL-6, TNF-α, and IL-1β. In this study, we investigated the anti-inflammatory effects of the Olea europaea extract from Jeju island using LPS which induces an inflammatory response. To examine the potential anti-inflammatory properties of the Olea europaea extract, we measured the amount of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and IL-1β on RAW 264.7 cells after the Olea europaea extract treatment. The Olea europaea extracts showed higher anti-inflammatory activity by inhibiting the production of PGE2, NO, TNF-α, IL-6, and IL-1β induced by LPS stimulation in RAW 264.7 cells. Also, Olea europaea extracts decreased mRNA expression of iNOS, and COX-2. There was no cytotoxicity in the macrophage proliferation treated with the Olea europaea extract compared to the control in the LDH assay. These results suggest that the Olea europaea extract may exert significant effects on inflammatory factors and be a potential source as a new natural anti-inflammatory agent. Further investigations will focus on cell-based in vitro assays and in chemically identifying the major active components mediating the anti-aging and anti-inflammation responses.
참고문헌 (30)
- Fang YZ, "Free radicals, antioxidants, and nutrition", Nutrition, 18, pp.872~879 (2002)[DOI]
- Bhattacharyya A, "Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases", Physiol Rev, 94, pp.329~354 (2014)[DOI]
- Rodriguez ML, "Natural polyphenols and apoptosis induction in cancer therapy", J Carcinog Mutagen, S6, pp.1~10 (2013)
- Saibandith B, "Olive Polyphenols and the Metabolic Syndrome", Molecules, 22, pp.1082~1095 (2017)[DOI]
- Ritchlin CT, "Mechanisms of TNF-<TEX>${\alpha}$<", TEX>- and RANKLmediated osteoclastogenesis and bone resorption in psoriatic arthritis, 2003, pp.111, 821~831
- Becker S, "Regulation of cytokine production in human alveolar macrophages and airway epithelial cells in response to ambient air pollution particles: further mechanistic studies", Toxicol Appl Pharmacol, 207, pp.S269~S275 (2005)[DOI]
- Pierce GF, "Macrophages: important physiologic and pathologic sources of polypeptide growth factors", Am J Respir Cell Mol Biol, 2, pp.233~234 (1990)[DOI]
- Simons RK, "Acute lung injury in endotoxemic rats is associated with sustained circulating IL-6 levels and intrapulmonary cinc activity and neutrophil recruitment-role of circulating TNF-<TEX>${\alpha}$<", TEX> and IL-<TEX>${\beta}$<, Shock, pp.1996, 6[DOI]
- Guslandi M, "Nitric oxide and inflammatory bowel disease", Eur J Clin Invest, 28, pp.904~907 (1998)[DOI]
- Hiraganahalli D, "Hepatoprotective and antioxidant activity of standardized herbal extracts", Pharmacogn Mag, 8, pp.116~123 (2012)[DOI]
- Gucci R, "Analysis of leaf water relations in leaves of two olive (Olea europea) cultivars differing in tolerance to salinity", Tree physiol, 17, pp.13~21 (1997)[DOI]
- Zarzuelo A, "Vasoliator effect of olive leaf", Planta Med, 57, pp.417~419 (1991)[DOI]
- Hertog MGL, "Dietary antioxidant flavonoids and risk of coronary heart disease", Lancet, 342, pp.1007~1011 (1993)[DOI]
- Aziz NH, "Comparative antibacterial and antifungal effects of some phenolic compounds", Microbios, 93, pp.43~54 (1998)
- Fernandez-Escobar R, "Seasonal changes of mineral nutrients in olive leaves during the alternate-bearing cycle", Sci Hortic, 82, pp.25~45 (1999)[DOI]
- Delgado-Pertinez M, "Predicting the nutritive value of the olive leaf (Olea europea): digestibility and chemical composition and in vitro studies", Anim Feed Sci Technol, 87, pp.187~201 (2000)[DOI]
- Bianco A, "Biophenolic components of olives", Food Res Int, 33, pp.475~485 (2000)[DOI]
- Tasioula-Margari M, "Isolation and characterization of virgin olive oil phenolic components by HPLC", UV and GC-MS, 2001, pp.66, 530~534
- Ryan D, "Biotransfomations of phenolic compounds in Olea europaea L", Sci Hortic, 92, pp.147~176 (2002)[DOI]
- Silva S, "Phenolic compounds and antioxidant activity of Olea europaea L. fruits and leaves", Food Sci Technol Int, 12, pp.385~395 (2006)[DOI]
- Nino AD, "Direct identification of phenolic glucosides from olive leaf extracts by atmospheric pressure Ionization tandem mass spectrometry", J Mass Spectrom, 32, pp.533~541 (1997)[DOI]
- Visioli F, "Antioxidant and other biological activities of phenols from olives and olive oil", Med Res Rev, 22, pp.65~75 (2002)[DOI]
- Del-Rio JA, "Enhancement of phenolic compounds in olive plants (Olea europaea L.) and their influence on resistance against Phytophthora sp.", Food Chem, 83, pp.75~78 (2003)[DOI]
- Espin JC, "Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2, 2-diphenyl-1-picrylhydrazyl radical", J Agric Food Chem, 48, pp.648~656 (2000)[DOI]
- Lee-Huang S, "Anti-Hiv activity of olive leaf extract (OLE) and modulation of host cell gene expression by HIV-1 infection and OLE treatment", Biochem Biophys Res Commun, 307, pp.1029~1037 (2003)[DOI]
- Delgado-Pertinez M, "Predicting the nutritive value of the olive leaf (Olea europaea): digestibility and chemical composition and in vitro studies", Anim Feed Sci Technol, 87, pp.187~201 (2000)[DOI]
- Lee ST, "Induction of nitric oxide and TNF-<TEX>${\alpha}$<", TEX> by herbal plant extracts in mouse macrophages, 2000, pp.29, 342~348
- 노경희, "RAW264.7 Macrophage Cell에서 녹차씨껍질 에틸아세테이트 분획의 염증억제 효과 및 기전 연구", 한국식품영양과학회지, 40(5), pp.625~634 (2011), 한국식품영양과학회
- Rocca B, "Cyclooxygenases and prostaglandins: shaping up the immune response", Int Immunopharmacol, 2, pp.603~630 (2002)[DOI]
- Turini ME, "Cyclooxygenase-2: a therapeutic target", Annu Rev Med, 53, pp.35~57 (2002)[DOI]