Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
Background: The accurate and rapid identification of the causative viruses is important for the timely diagnosis and management of respiratory infections. Multiplex molecular diagnostic techniques have been widely adopted to detect respiratory viruses. We compared the results of a newly upgraded, multiplex, molecular bead-based respiratory viral panel (RVP) assay with the results of Anyplex II RV16 detection kit and AdvanSure RV real-time RT-PCR assay. Methods: We tested 254 respiratory specimens and cultured viral strains using the Luminex xTAG RVP Fast v2 assay (Luminex Molecular Diagnostics, Canada) and Anyplex II RV16 detection kit and compared the results. Specimens showing discordant results between the two assays were tested with a AdvanSure RV real-time RT-PCR assay. Results: Of the 254 respiratory specimens, there was total agreement in the results between the xTAG RVP Fast v2 assay and the other real-time PCR assay in 94.1–100% of the specimens. The agreement levels were relatively low (94.1–97.6%) for specimens of adenovirus, coronavirus NL63, and parainfluenza type 3. In comparison to the other assay, the xTAG RVP Fast v2 assay detected a higher number of parainfluenza type 3 (4 cases) and metapneumovirus (9 cases). Conclusions: The xTAG RVP Fast v2 assay showed comparable capabilities compared with the other assays; it will be useful for identifying respiratory viral infections in patients with respiratory symptoms. Clinicians should be aware of the characteristics of the assays they use, since different assays show different detectability for each virus.
Abstract
Background: The accurate and rapid identification of the causative viruses is important for the timely diagnosis and management of respiratory infections. Multiplex molecular diagnostic techniques have been widely adopted to detect respiratory viruses. We compared the results of a newly upgraded, multiplex, molecular bead-based respiratory viral panel (RVP) assay with the results of Anyplex II RV16 detection kit and AdvanSure RV real-time RT-PCR assay. Methods: We tested 254 respiratory specimens and cultured viral strains using the Luminex xTAG RVP Fast v2 assay (Luminex Molecular Diagnostics, Canada) and Anyplex II RV16 detection kit and compared the results. Specimens showing discordant results between the two assays were tested with a AdvanSure RV real-time RT-PCR assay. Results: Of the 254 respiratory specimens, there was total agreement in the results between the xTAG RVP Fast v2 assay and the other real-time PCR assay in 94.1–100% of the specimens. The agreement levels were relatively low (94.1–97.6%) for specimens of adenovirus, coronavirus NL63, and parainfluenza type 3. In comparison to the other assay, the xTAG RVP Fast v2 assay detected a higher number of parainfluenza type 3 (4 cases) and metapneumovirus (9 cases). Conclusions: The xTAG RVP Fast v2 assay showed comparable capabilities compared with the other assays; it will be useful for identifying respiratory viral infections in patients with respiratory symptoms. Clinicians should be aware of the characteristics of the assays they use, since different assays show different detectability for each virus.
참고문헌 (21)
- Lim FJ, "Systematic review and meta-analysis of respiratory viral coinfections in children", Respirology, 21, pp.648~655 (2016)
- Lee KH, "The role of respiratory viruses in the etiology of bacterial pneumonia: an ecological perspective", Evol Med Public Health, 2016, pp.95~109 (2016)
- Manuel O, "Influenza and other respiratory virus infections in solid organ transplant recipients", Clin Microbiol Infect, (20 Suppl 7), pp.102~108 (2014)
- Garbino J, "Lower respiratory viral illnesses: improved diagnosis by molecular methods and clinical impact", Am J Respir Crit Care Med, 170, pp.1197~1203 (2004)
- Kanashiro TM, "Identification of respiratory virus in infants with congenital heart disease by comparison of different methods", Rev Inst Med Trop Sao Paulo, 53, pp.241~246 (2011)
- Charles PG, "Early diagnosis of lower respiratory tract infections (point-of-care tests)", Curr Opin Pulm Med, 14, pp.176~182 (2008)
- Chartrand C, "Accuracy of rapid influenza diagnostic tests: a meta-analysis", Ann Intern Med, 156, pp.500~511 (2012)
- Mahony JB, "Molecular diagnosis of respiratory virus infections", Crit Rev Clin Lab Sci, 48, pp.217~249 (2011)
- Ginocchio CC, "Strengths and weaknesses of FDA-approved", cleared diagnostic devices for the molecular detection of respiratory pathogens, 2011, pp.52 Suppl 4, S312~S325
- Duong VT, "An evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel assay for the detection of multiple diarrheal pathogens in fecal samples in Vietnam", J Clin Microbiol, 54, pp.1094~1100 (2016)
- Vilches M, "Analysis of Pregnancy-Induced Anti-HLA Antibodies Using Luminex Platform", Transplant Proc, 47, pp.2608~2610 (2015)
- Kim HK, "Comparison of Anyplex II RV16 with the xTAG respiratory viral panel and Seeplex RV15 for detection of respiratory viruses", J Clin Microbiol, 51, pp.1137~1141 (2013)
- Dabisch-Ruthe M, "Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay", BMC Infect Dis, 12, pp.163~ (2012)
- Mahony J, "Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay", J Clin Microbiol, 45, pp.2965~2970 (2007)
- Popowitch EB, "Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP fast multiplex assays for detection of respiratory viruses", J Clin Microbiol, 51, pp.1528~1533 (2013)
- McHugh ML, "Interrater reliability: the kappa statistic", Biochem Med (Zagreb), 22, pp.276~282 (2012)
- Walsh MP, "Computational analysis of two species C human adenoviruses provides evidence of a novel virus", J Clin Microbiol, 49, pp.3482~3490 (2011)
- 김자영, "Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples", Annals of Laboratory Medicine, 34(3), pp.216~222 (2014), 대한진단검사의학회
- Gharabaghi F, "Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children", Clin Microbiol Infect, 17, pp.1900~1906 (2011)
- Jung YJ, "Comparison of the AdvanSure real-time RT-PCR and Seeplex((R)) RV12 ACE assay for the detection of respiratory viruses", J Virol Methods, 224, pp.42~46 (2015)
- Huh HJ, "Comparison of the Anyplex(TM) II RV16 and Seeplex((R)) RV12 ACE assays for the detection of respiratory viruses", Diagn Microbiol Infect Dis, 79, pp.419~421 (2014)