Evaluation of randomly amplified polymorphic DNA-polymerase chain reaction method for the identification of eighteen lyophilized probiotics commercially available
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Identification of probiotics is crucial to ensure the quality of food products manufactured at the industrial scale. Although various techniques have been introduced for bacterial identification, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) is generally accepted as a conventional method for bacterial identification. In this study, RAPD-PCR method was evaluated for the identification of eighteen commercial probiotic strains. In results, cellular and product (lyophilized) forms of each probiotic strain were successfully identified based on band intensities and size of the amplified genes. Therefore, RAPD-PCR method may be applied for the identification of lyophilized probiotics commercially available.
Abstract
Identification of probiotics is crucial to ensure the quality of food products manufactured at the industrial scale. Although various techniques have been introduced for bacterial identification, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) is generally accepted as a conventional method for bacterial identification. In this study, RAPD-PCR method was evaluated for the identification of eighteen commercial probiotic strains. In results, cellular and product (lyophilized) forms of each probiotic strain were successfully identified based on band intensities and size of the amplified genes. Therefore, RAPD-PCR method may be applied for the identification of lyophilized probiotics commercially available.
참고문헌 (15)
- Booysen C, "Isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barley cultivars", Int J Food Microbiol, 76, pp.63~73 (2002)
- de Simone C, "The unregulated probiotic market", Clin Gastroenterol Hepatol, 17, pp.809~817 (2019)
- Granato D, "Probiotic dairy products as functional foods", Compr Rev Food Sci Food Safety, 9, pp.455~470 (2010)
- Jarocki P, "Molecular routes to specific identification of the Lactobacillus casei group at the species, subspecies and strain level", Int J Mol Sci, 21, pp.2694~ (2020)
- Johansson ML, "Randomly amplified polymorphic DNA(RAPD)for rapid typing of Lactobacillus plantarum strains", Lett Appl Microbiol, 21, pp.155~159 (1995)
- Kechagia M, "Health benefits of probiotics : A review", ISRN Nutr, 2013, pp.481651~ (2013)
- Mohania D, "Molecular approaches for identification and characterization of lactic acid bacteria", J Dig Dis, 9, pp.190~198 (2008)
- Schillinger U, "Use of group-specific and RAPD-PCR analyses for rapid differentiation of Lactobacillus strains from probiotic yogurts", Curr Microbiol, 47, pp.453~456 (2003)
- Sesena S, "Genetic diversity(RAPD-PCR)of Lactobacilli isolated from"Almagro"eggplant fermentations from two seasons", FEMS Microbiol Lett, 238, pp.159~165 (2004)
- Sharma A, "Tracking of intentionally inoculated lactic acid bacteria strains in yogurt and probiotic powder", Microorganisms, 8, pp.5~ (2020)
- Tabit FT, "Advantages and limitations of potential methods for the analysis of bacteria in milk : A review", J Food Sci Technol, 53, pp.42~49 (2016)
- Temmerman R, "Identification of lactic acid bacteria : Culture-dependent and culture-independent methods", Trends Food Sci Technol, 15, pp.348~359 (2004)
- Tilsala-Timisjarvi A, "Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers", Appl Environ Microbiol, 64, pp.4816~4819 (1998)
- Williams JGK, "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Res, 18, pp.6531~6535 (1990)
- Yu J, "Rapid identification of lactic acid bacteria isolated from home-made fermented milk in Tibet", J Gen Appl Microbiol, 55, pp.181~190 (2009)