Random Amplification of Polymorphic DNA와 혈청학적 분석을 이용한 국내식품에서 분리한 Listeria monocytogenes의 분류
Classification of Listeria monocytogenes Isolates from Korean Domestic Foods Using Random Amplifi-cation of Polymorphic DNA and Serotyping Analysis
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Suwon 449-701, Korea − Molecular subtyping of Listeria monocytogenes, including type strains and isolates from Korean foods, were performed using random amplification of polymorphic DNA (RAPD). Each Listeria species showed specific RAPD band patterns, and L. monocytogenes serotypes and isolates were divided into two clusters. RAPD results showed that L. monocytogenes isolates from Korean foods were divided into two groups. Group I contained L. monocytogenes serotypes 1/2b and 4b, whereas Group II contained serotypes 1/2a and 1/2c. These results suggested RAPD as possible subtyping methods for Listeria species. Also, RAPD Results showed significant correlation between molecular subtyping and serotyping of L. monocytogenes, and classified two different groups of L. monocytogenes isolated from Korean foods.
Abstract
Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Suwon 449-701, Korea − Molecular subtyping of Listeria monocytogenes, including type strains and isolates from Korean foods, were performed using random amplification of polymorphic DNA (RAPD). Each Listeria species showed specific RAPD band patterns, and L. monocytogenes serotypes and isolates were divided into two clusters. RAPD results showed that L. monocytogenes isolates from Korean foods were divided into two groups. Group I contained L. monocytogenes serotypes 1/2b and 4b, whereas Group II contained serotypes 1/2a and 1/2c. These results suggested RAPD as possible subtyping methods for Listeria species. Also, RAPD Results showed significant correlation between molecular subtyping and serotyping of L. monocytogenes, and classified two different groups of L. monocytogenes isolated from Korean foods.
참고문헌 (13)
- "Use of automatedribotyping of Austrian Listeria monocytogenes isolates tosupport epidemiological typing", 35, pp.237~244 (1999)
- "Randomamplification of polymorphic DNA typing of Listeriamonocytogenes isolated from meat", 69, pp.227~235 (2001)
- "Developmentand evaluation of a 24-hour method for the detection andquantification of Listeria monocytogenes in meat product", 63, pp.347~353 (1999)
- "Amplified Fragment length polymorphism (AFLP) analysisof Listeria monocytogenes. System", 25, pp.456~461 (2002)
- "RAPD analysis, serotyping and esterase typingindicate that the population of Listeria monocytogenes strainsrecoverd from cheese and from patients with listeriosis inBelgium are different", 47, pp.883~887 (2001)
- "Geneticvariability among isolate of Listeria monocytogenes fromfood products estimated by RAPD typing", 84, pp.285~297 (2003)
- "Combined ribotyping and randommultiprimer DNA analysis to probe the population structureof Listeria monocytogenes", 68, pp.2849~2856 (2002)
- "Correlation between molecularsubtyping and serotyping of Listeria monocytogenes", 39, pp.2704~2707 (2001)
- "Application ofmultilocus enzyme electrophoresis in studies of theepidemiology of Listeria monocytogenes in Denmark", 59, pp.2817~2822 (1993)
- "Rapid identification ofListeria species by using restriction fragment lengthpolymorphism of PCR-amplified 23S rRNA gene fragments", 69, pp.6386~6392 (2003)
- "Listeria monocytogenes exists in at least threevolutionary lines", 141, pp.2053~2061 (1995)
- "Restriction fragmentlength polymorphism in mitochondrial DNAs of Armillaria", pp.247~256 (1989)
- "Analysis ofsurface proteins of Listeria in relation to species" (1992)