생육 시기에 따른 피자두(Prunus salicina Lindl. Cv. Soldam)의 항산화 효과
Antioxidant Activities of Prunus salicina Lindl. cv. Soldam (Plum) at Different Growth Stages
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초록
본 연구에서는 자두류 중 피자두를 생육시기별로 채취하여 총 8종에 대한 일반성분 및 총 페놀성 화합물, 축합형 탄닌 함량을 조사하였으며, DPPH 자유 라디칼 소거능과 linoleic acid에 대한 항산화 효과를 살펴보았다. 피자두 과육의 수분함량 및 아세톤추출물의 조회분, 조단백, 조지방 함량을 분석한 결과, 시료 1, 5, 8번은 각각 수분 88.52%, 87.01%, 83.56%, 조회분 7.12%, 3.35%, 3.57%, 그리고 조단백 7.52%, 5.55%, 3.85%, 및 조지방 3.20%, 0.99%, 5.15%로 나타났다. 또한 피자두 아세톤 추출물의 총 폴리페놀 함량은 시료 1, 5, 8번이 각각 10.67%, 4.05%, 2.57%로 나타났으며, 축합형 탄닌 함량은 각각 8.36%, 3.11%, 1.88%로 나타났다. 항산화 효과를 알아보기 위해 DPPH free radical의 소거활성법을 측정한 결과, 피자두 추출물 1과 2의 RC50값이 각각 2.23과 9.70 μg/mL로 가장 높은 소거활성을 보였으며 피자두가 완숙될수록 소거활성은 점차적으로 감소하였다. Linoleic acid를 기질로 한 피자두 추출물의 산화방지 효과를 FTC 법과 TBA 법으로 측정한 결과, 초기 지질산화를 나타내는 FTC 가에서는 피자두의 미숙과 추출물 처리구의 경우 85% 이상의 산화 억제율을 보였고, 후기 지질산화에 관여하는 TBA 가는 미숙과 추출물에서 85% 이상의 산화 억제율을 보였으나 피자두가 완숙될수록 산화 억제율은 감소하였다.
Abstract
This study was performed to evaluate the antioxidant effect of Prunus salicina Lindl. cv. Soldam at different growth stages (sample 1~8). Previous studies shows that this fruits possess hematopoiesis effect, osteoporosis prevention, and antimutagenic effects. Prunus salicina Lindl. cv. soldam was picked in every 5 days from the 40th day before harvesting date for marketing in Gimcheon, Gyeongbuk. The fruits at different growth stages (sample 1~8) were extracted with 60% acetone and chlorophyll in the extracts was removed. In proximate compositions, the contents of moisture of sample 1, 5, 8 were 88.52, 87.01, 83.56%；crude ash were 7.12, 3.35, 3.57%；crude protein were 7.52, 5.55, 3.85%；crude fat contents were 3.20, 0.99, 5.15%, respectively. The contents of total polyphenols and condensed tannin in the acetone extracts from sample 1, 5, 8 were 10.67, 4.05, 2.57%, and 8.36, 3.11, 1.88%, respectively. The antioxidantive effect of acetone extracts from immature fruits showed strong scavenging effect on DPPH free radicals. The RC50 values of the extracts from sample 1, 2 were 2.23, 9.70 ㎍/mL, respectively while those of butylated hydroxyanisole (BHA) was 5.25 ㎍/mL. The extracts from immature fruits showed over 85% inhibition on peroxidation of linoleic acid at 100 ㎍/mL as determined by both the ferric thiocyanate (FTC) and the thiobarbituric acid (TBA) method.
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