솔잎 열수 증류액의 생리활성 효과
Biological Activities of the Vaporized Liquid of Water-boiled Pine Needle
0
조회
0
다운로드
5
인용
0
참고문헌
초록
솔잎 열수 증류액에 대한 항산화 활성은 RC50값이 18.4 μL로서 매우 강한 항산화 활성을 나타내었다. 솔잎 열수 증류액에 대한 항돌연변이 활성은 S. typhimurium TA98과 TA100 균주를 이용한 돌연변이성 실험 결과 솔잎 열수 증류액 자체로서는 변이원성이 없었으며 항돌연변이원성 실험에서는 직접변이원인 MNNG (0.4 μL/plate)의 경우 S. typhimurium TA100 균주에서 시료농도 200 μL/plate에서 45.9%의 억제활성을 나타내었으며 4NQO (0.15 μg/plate)에 대해서는 동일 농도에서 85.5%의 높은 억제활성을 나타내었다. 그리고 간접변이원인 B(α)P (10 μg/plate)에 대해서는 시료농도 200 μg/plate의 시료농도에서 88%의 높은 억제 활성을 나타내었다. 한편 Trp-P-1 (0.15 μg/plate)에 대해서는 50 μg/plate의 낮은 시료농도에서도 85%의 높은 억제활성을 나타내었으며 모두 농도 의존적으로 억제하는 것으로 나타났다. 솔잎 열수 증류액의 암세포 성장억제효과를 검토한 결과, 폐암세포, 유방암세포에 대해서 각각 78.7%, 62.3%의 비교적 높은 억제효과를 보였으며, 특히 위암세포, 자궁암세포, 간암세포에서 각각 90.8%, 93.7%, 90.0%의 높은 억제효과를 나타내었다.
Abstract
This study was performed to determine the antioxidative, antimutagenic, and anticancer effects of vaporized liquid of water-boiled pine needle(VLP) using DPPH free radical donating method, Ames test, and cytotoxicity. VLP showed the highest electron donating activities (18.4 μL). The inhibition rate of VLP (200 μL/plate) in the Salmonella. typhimurium TA100 strain showed 45.9% inhibition against the mutagenesis induced by MNNG. In addition, the suppression of with same concentration of VLP in the S. typhimurium TA100 strains showed 85.5% inhibition against 4NQO, respectively. The suppressions under the same condition against Trp-P-1 in the TA98 and TA100 strains were 91.0% and 62.1%, respectively. The cytotoxic effects of VLP against the cell lines with human lung carcinoma (A549), human hepatocellular carcinoma (HepG2), human gastric carcinoma (AGS), human breast adenocarcinoma (MCF-7) and human cervical adenocarcinoma (HeLa) were inhibited with increase of the VLP concentration. The treatment of 50 μL/well VLP showed strong cytotoxicities of 78.7%, 90.3%, 90.8%, 62.3% and 93.7% against A549, HepG2, AGS, MCF-7 and HeLa, respectively.
참고문헌 (15)
- "食用植物中の生理的機能成分", 食品と開發, pp.27~29 (1992)
- "Studies on the physiological functionality of pine needle and mugwort extracts", 27, pp.978~984 (1995)
- "Phytochemicals and antioxidants. In functional Foods" (1994), New York
- "Prevention of cancer by agents that suppress production of oxidants. In Food Phytochemicals for cancer Prevention", pp.547~ (1994)
- "신비의 솔잎치료법", pp.21~ (1995), 上原美鈴
- "약초의 성분과 이용", pp.114~116 (1991), 일월서각 서울
- "Effects of dietary supplementation with pine leaf on lipid parameters in rat", pp.47~50 (1991)
- "Antimicrobial activites of pine needle(pinus denesiflora seid et Zucc.) extract", 25, pp.293~297 (1997)
- "Cytotoxic effect of the pine needle extracts", 30, pp.213~217 (1998)
- -furanose from pin needles as an antioxiative principle, , 1994, 310~314
- "Screening for antioxidant activity of plants and marine algae and its active principles from Prunus daviana. Kor. J. Pharmacol.", pp.299~303 (1993)
- "Mutagenicities of N-nitrosoamines on Salmonella.", 48, pp.121~130 (1997)
- "Evaluation of a soluble tetrazolium", formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res., 1988, 4827~4836
- "Inhibitory effect of main pine needle extracts on the chemically induced mutagenicity", 30, pp.450~455 (1998)
- "Cytotoxic effect of the distilled pine-needle extracts on several cancer cell lines in vitro", 31, pp.691~695 (2002)