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발효 콩을 주원료로 한 기능성 식품 추출물의 항산화성, 항동연변이원성 및 세포독성 효과

Antioxidative, Antimutagenic and Cytotoxic Effects of Functional Food Manufactured from Fermented Soybean Extract

박종철|최승필|오흥석|이득식|함승시|남기본
한국식품저장유통학회 (The Korean Society of Food Preservation)|2004.06|Food Science and Preservation|제11권 제2호|pp. 214-220

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참고문헌

주제분류식품과학

초록

발효콩을 주원료로 한 기능성 식품의 에탄올 추출물과 분획물에 대한 항산화 활성을 측정한 결과, 에틸아세테이트 분획물에서 31.6 μg/mL의 강한 항산화 활성을 나타내었다. 직접 변이원인 MNNG에 대한 항돌연변이 효과에서 S. typhimurium TA100 균주에 대해 발효 콩을 주원료로 한 기능성 식품의 에탄올 추출물(200 μg/plate)에서 84.8%의 억제효과를 나타내었다. Trp-P-1에 대해서는 S. typhimurium TA98과 TA100 두 균주에 대해 각각 88.7%와 92.8%로 다른 분획물보다 높은 억제효과를 나타내었다. 암세포 성장억제 효과를 검토한 실험에서는 A549와 AGS 및 MCF-7의 경우 에틸아세테이트 분획물이 가장 높은 억제활성을 나타내었으며, 시료농도의 증가와 함께 억제활성도 증가하는 경향을 나타내었으며 시료농도 1 mg/mL에서 A549에 대해서 84.5%와 AGS에서는 88.7% 그리고 MCF-7에 대해서는 85.6%의 높은 암세포 성장억제효과를 나타내었다.

Abstract

This study was carried out to determine the antioxidative, antimutagenic, and anticancer effects of functional food manufactured from fermented soybean(FFMFS) using DPPH free radical donating method, Ames test and cytotoxicity, respectively. FFMFS extracted with ethanol and then further fractionated to n-hexane, chloroform, ethyl acetate(EtOAc), butanol and water, stepwise. Among five fractions, the EtOAc fractions showed highest electron donating activities (31.6 ㎍/mL). The inhibition rate of ethanol extract(200 μg/plate) of FFMFS in the S. typhimurium TA100 strain showed 84.8% against the mutagenesis induced by MNNG. In addition, the suppression of EtOAc fractions with same concentration of FFMFS the S. typhimurium TA98 and TA100 strains showed 88.7% and 92.8% inhibition against Trp-P-1, respectively. The cytotoxic effects of FFMFS against the cell lines with human lung carcinoma(A549), human gastric carcinoma(AGS) and human breast adenocarcinoma(MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL FFMFS of EtOAc fraction showed strong cytotoxicities of 84.5%, 88.7% and 85.6% against A549, AGS and MCF-7, respectively.

참고문헌 (20)

  1. "Phytochemicals and antioxidants. In functional Foods" (1994)
  2. "Prevention of cancer by agents that suppress production of oxidants. In Food Phytochemicals for cancer Prevention", pp.547~ (1994)
  3. "Increasing use of soyfoods and their potential role in cancer prevention. J. Am. Diet. Assoc.", pp.836~841 (1991)
  4. "Health effects of oligosaccharides. Food Technol.", pp.61~67 (1994)
  5. "Antioxidative characteristics of melanoidin related products fractionated from fermented soybean sauce. J. Kor. Soc. Food Nutr.", pp.570~575 (1993)
  6. "Antimutagenic effect of doenjang", pp.156~162 (1990)
  7. "Cytotoxity of fermented soybean products with various tumor cell using MTT assay. Kor. J. Appl. Microbool. Biotechnol.", pp.477~482 (1997)
  8. "Antitumor isoflavones in soybean foods from american and asian diets. J. Agric. Food Chem."
  9. "Screening for antioxidant activity of plants and marine algae and its active principles from prunus daviana. Kor. J. Pharmacol.", pp.299~303 (1993)
  10. "Mutagenicities of N-nitrosoamines on salmonella. Mutation Res.", sei, pp.121~130 (1997)
  11. "Evaluation of a soluble tetrazolium", formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res., 1988, 4827~4836
  12. "Estrogenic and antiproliferative propertis of genistein and other flavonoids in human breast cancer cells in vitro. Nutr. Cancer", pp.31~40 (1997)
  13. "Antimutagenic and anticancer effects of ethanol extract from korean traditional doenjang added sea tangle. J. Kor. Soc. Food Sci. Nutr.", pp.322~328 (2002)
  14. "Antimutagenicity and cytotoxic effect of ethanol extract from korean traditional mackjang added sea tangle. J. East Asian Soc. Dietary Life", pp.15~22 (2002)
  15. "Composition and antioxidative characteristics of phenolic fractions isolated from soybean fremented food. J. Kor. Soc. Food Sci. Nutr.", pp.383~389 (1997)
  16. "Antimutagenic effect of doengjang-inhibitory effect of doengjang stew and soup on the mutagenicity induced by aflatoxin B1 environ. Mutag. Carcin.", pp.145~152 (1994)
  17. "areview of the in vitro and in vivo date. Nutr. Cancer", pp.113~131 (1994)
  18. "Epidemiologic correlation between diet and cancer frequency. Cancer Res.", pp.3685~3690 (1981)
  19. "Role of life-style and dietary habits in risk of cancer among seventh day adventists. Cancer Res.", pp.3515~3522 (1975)
  20. "Growth suppression effect of traditional fermented soybean paste", pp.458~463 (1999)

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