해바라기씨 추출물의 천식 완화 효과
Water-extract of Helianthus annus Seed Exhibits Potent Anti-asthma Activity In Vitro and In Vivo
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초록
항산화물에 의한 천식 억제 효과를 확인하기 위하여 항산화정도를 알아볼 수 있는 DPPH, FRAP, hydroxyl radical 소거활성 실험을 high throughput-compatible chemical assay로 디자인하여 해바라기씨 추출물에 적용하여 보았다. 해바라기씨 추출물의 용매에 따른 항산화 효과 중 H2O2에 의한 산화스트레스에 의한 세포사멸을 DW추출물에서 강한 억제능력을 보였다. 이를 이용하여 마우스를 이용한 천식 동물모델에 적용하여 탁월한 천식 억제효과를 CD4+세포, IL-4와 IL-13의 발현정도를 통해 알 수 있었다. 해바라기씨 추출물은 천식에 탁월한 효과가 있는 것으로 in vitro 및 in vivo 실험으로 증명하였다.
Abstract
In order to investigate whether antioxidant biomaterials inhibits IL-4 and/or IL-13 expression in vitro and in vivo, we carried out antioxidant assays by enzyme or cell-based assays with Helianthus annuus extract. Antioxidant assays include DPPH, FRAP, hydroxyl radical assays. Helianthus annuus extract exhibited SOD scavenging activity, and had different patterns by each solvent extracted reaction. DW extract inhibited oxidative stress by H2O2 that induced apoptosis. We measured CD4+ cell and IL-4/13 cytokine expression in a classical mouse animal model. The results show that Helianthus annuus extract showed strong inhibition of immune response in the lung. These results suggest that Helianthus annuus extract can reduce inflammation induced by a mouse asthma model.
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