약용곤충 무당벌레류 추출물의 항산화활성과 Cyclooxygenase-2 Promoter 억제활성 비교
Comparison of In Vitro Antioxidant Activity and Cyclooxygenase-2 Promoter Inhibitory Activity in Harmonia axyridis Pallas and Coccinella septempunctata Linne
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초록
최근 곤충자원은 약용 및 식용으로 활용하려고 많은 연구가 되고 있으며 미래의 자원으로 발전할 수 있는 가능성은 충분하다. 본 연구는 곤충자원 중 칠성무당벌레와 무당벌레를 이용하여 생물학적 활성과 함께 약용 및 식용으로의 활용가능성을 알아보고자 하였다. 생물학적 활성을 알아보기 위하여 이들 추출물을 이용하여 항산화관련 실험인 DPPH, FRAP, linoleic acid 산화 억제실험을 하였으며, 분자염증에 관련된 유전자인 Cyclooxygenase-2(Cox-2)의 promoter의 유전자 발현의 억제 유무를 알아보았다. 항산화 효과에 있어서는 무당벌레의 DW추출물에서 높은 활성을 나타내었으며, Cox-2의 promoter 활성을 조사한 결과 DW추출물에서 Cox-2의 promoter 활성을 약 25% 정도 억제하는 무당벌레의 DW추출물을 발견하였는데 추출물의 산업적인 활용가능성을 확인하기 위하여 보다 많은 연구가 필요하다.
Abstract
Insect resources have been widely recognized that several millions of insects exhibit its own biological activity by whole body or its metabolic intermediates. In order to investigate antioxidant activity and compare the cyclooxygenase-2 promoter activity from insect extracts, we tested in vitro antioxidant assays and cyclooxygenase-2 promoter assay in Coccinella septempunctata Linn and Harmonia axyridis Pallas, for possible medicinal food biomaterials. To determine whether Coccinella septempunctata Linn and Harmonia axyridis extracts have the anti-oxidant and cyclooxygenase-2 inhibition activities, we examined the anti-oxidant assays including DPPH, FRAP and linoleic acid, and, inhibition of cyclooxygenase-2 expression using a cyclooxygenase-2 promoter-inserted stable cell line. We found that Harmonia axyridis Pallas extract had potentials to anti-oxidant activity and inhibited about 25% of cyclooxygenase-2 transcription activity. These findings indicate that Coccinella septempunctata Linn and Harmonia axyridis Pallas extracts could be an useful insect resource for agrobiotechnological purposes.
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