Enzyme hydrolysate of silk protein suppresses tert-butyl hydroperoxide-induced hepatotoxicity by enhancing antioxidant activity in rats
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The purpose of current study is to investigate the beneficial effect of enzyme (Alcalase) hydrolysates of silk protein in rat. Alcalase-treated silk protein hydrolysate (ATSH) itself did not show any cytotoxicity on the hepatic tissues and blood biochemistry, similar to the normal condition. ATSH played a protective role in tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity and liver damage. The values of AST (aspartate aminotransferase) and ALT (alanine aminotransferase), which are the indicators of the liver function, were effectively alleviated with the ATSH treatment in a dose dependent manner. The level of Lactate dehydrogenase (LDH) and Malondialdehyde (MDA), which were increased with t-BHP treatment, were significantly reduced by ATSH. High dose of ATSH (2 g/kg) reduced the t-BHP-induced LDH release by 48%. Antioxidant and antioxidant enzymes in liver cells were significantly increased by ATSH treatment in their level and activities. ATSH (2 g/kg) increased glutathione (GSH), an intracelluar antioxidant, by 2.5-fold compared with the t-BHP treated group. The activities of glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase were also elevated by 38%, 60%, and 45%, respectively, with ATSH (2 g/kg) treatment. The antioxidative effect of ATSH was recapitulated to the protection from t-BHP induced liver damages in hematoxylin and eosin (H&E) staining. Thus, ATSH might be used as a hepatoprotective agent.
Abstract
The purpose of current study is to investigate the beneficial effect of enzyme (Alcalase) hydrolysates of silk protein in rat. Alcalase-treated silk protein hydrolysate (ATSH) itself did not show any cytotoxicity on the hepatic tissues and blood biochemistry, similar to the normal condition. ATSH played a protective role in tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity and liver damage. The values of AST (aspartate aminotransferase) and ALT (alanine aminotransferase), which are the indicators of the liver function, were effectively alleviated with the ATSH treatment in a dose dependent manner. The level of Lactate dehydrogenase (LDH) and Malondialdehyde (MDA), which were increased with t-BHP treatment, were significantly reduced by ATSH. High dose of ATSH (2 g/kg) reduced the t-BHP-induced LDH release by 48%. Antioxidant and antioxidant enzymes in liver cells were significantly increased by ATSH treatment in their level and activities. ATSH (2 g/kg) increased glutathione (GSH), an intracelluar antioxidant, by 2.5-fold compared with the t-BHP treated group. The activities of glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase were also elevated by 38%, 60%, and 45%, respectively, with ATSH (2 g/kg) treatment. The antioxidative effect of ATSH was recapitulated to the protection from t-BHP induced liver damages in hematoxylin and eosin (H&E) staining. Thus, ATSH might be used as a hepatoprotective agent.
참고문헌 (37)
- Kim J, "Silk peptide treatment can improve the exercise performance of mice", J Int Soc Sports Nutr, 11, pp.35~ (2014)
- Lee SH, "Physiologic functionality of silk peptides-focus on antioxidant and immune function", Food Science and Industry, 35, pp.57~61 (2002)
- Hwang E, "Protein quality evaluation and effect of plasma lipid contents of acid hydrolysates of cocoon in rats fed by high cholesterol, high triglyceride and high sucrose diet", J Korean Soc Food Sci Nutr, 30, pp.1004~1009 (2001)
- Zhaorigetu S, "Inhibitory effects of silk protein, sericin on UVB-induced acute damage and tumor promotion by reducing oxidative stress in the skin of hairless mouse", J Photochem Photobiol B, 71, pp.11~17 (2003)
- 김애정, "뽕잎분말이 고콜레스테롤 식이 투여 흰쥐의 지질대사에 미친 영향", 한국식품과학회지, 37(4), pp.636~641 (2005), 한국식품과학회
- 김대원, "Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model", BMB Reports, 44(12), pp.787~792 (2011), 생화학분자생물학회
- Abdel-Misih SRZ, "Liver anatomy", Surg Clin N Am, 90, pp.643~653 (2010)
- Davis GL, "Projecting future complications of chronic hepatitis C in the United States", Liver Transplant, 9, pp.331~338 (2003)
- Cesaratto L, "The importance of redox state in liver damage", Ann Hepatol, 3, pp.86~92 (2004)
- Baud L, "Involvement of reactive oxygen species in kidney damage", Br Med Bull, 49, pp.621~629 (1993)
- Griffiths HR, "The selective protection afforded by ebselen against lipid peroxidation in an ROS-dependent model of inflammation", Agents Actions, 36, pp.107~111 (1992)
- Halliwell B, "Free radicals, antioxidants, and human", J Lab Clin Med, 119, pp.598~620 (1992)
- McCord JM, "Superoxide", Free Radic Biol Med, 5, pp.363~369 (1988)
- Meister A, "Glutathione metabolism and its selective modification", J Biol Chem, 263, pp.17205~17208 (1988)
- Spector A, "Review : Oxidative stress and disease", J Ocul Pharmacol Ther, 16, pp.193~201 (2009)
- Kim EY, "Protective effects of germinated and fermented soybean extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats", Food Funct, 6, pp.3512~3521 (2015)
- Lock JF, "The costs of postoperative liver failure and the economic impact of liver function capacity after extended liver resection-a single-center experience", Langenbecks Arch Surg, 394, pp.1047~1056 (2009)
- 김주현, "실크 단백질 가수분해물의 간 손상에 대한 보호효과", 한국식품저장유통학회지, 24(1), pp.107~115 (2017), 한국식품저장유통학회
- Wang CJ, "Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats", Food Chem Toxicol, 38, pp.411~416 (2000)
- Reed DJ, "Regulation of reductive processes by glutathione", Biochem Pharmacol, 35, pp.7~13 (1986)
- Habig WH, "Glutathione", J Biol Chem, 249, pp.7130~7139 (1974)
- Aebi H, "Activity and stability of catalase in blood and tissues of normal and acatalasemic mice", Biochem Genet, 2, pp.245~251 (1968)
- Durackova Z, "Superoxide dismutase mimetic activity of macrocyclic Cu(II)-tetraanhydroamin-obenzaldehyde(TAAB)complex", J Inorg Biochem, 58, pp.297~303 (1995)
- Lee HS, "Antioxidant effects of aqueous extract of Terminalia chebula in vivo and in vitro", Biol Pharm Bull, 28, pp.1639~1644 (2005)
- Cassidy WM, "Serum lactic dehydrogenase in the differential diagnosis of acute hepatocellular injury", J Clin Gastroenterol, 19, pp.118~121 (1994)
- Chiang WD, "Lipolysis stimulating peptides of potato protein hydrolysate effectively suppresses high-fat-diet-induced hepatocyte apoptosis and fibrosis in aging rats", Food Nutr Res, 60, pp.31417~ (2016)
- 정용일, "알코올성 간 손상을 유발한 흰쥐에 대한 고 분지아미노산 함유 옥수수 단백가수물의 간 기능 보호효과", 한국식품과학회지, 41(6), pp.706~711 (2009), 한국식품과학회
- Kamoun Z, "Hepatoprotective and nephroprotective effects of sardinelle(Sardinella aurita)protein hydrolysate against ethanol-induced oxidative stress in rats", Environ Sci Pollut Res Int, 24, pp.1432~1441 (2017)
- Draper HH, "Malondialdehyde determination as index of lipid Peroxidation", Method Enzymol, 186, pp.421~431 (1990)
- Han BK, "Hepatoprotective effects of soluble rice protein in primary hepatocytes and in mice", J Sci Food Agric, 96, pp.685~694 (2016)
- Cai X, "In vitro antioxidant activities of enzymatic hydrolysate from Schizochytrium sp. and its hepatoprotective effects on acute alcohol-induced liver injury in vivo", Mar Drugs, 15, pp.115~ (2017)
- Lushchak VI, "Glutathione homeostasis and functions: potential targets for medical interventions", J Amino Acids, 2012, pp.736837~ (2012)
- Nasri R, "Ameliorating effects of goby fish protein hydrolysates on high-fat-high-fructose diet-induced hyperglycemia, oxidative stress and deterioration of kidney function in rats", Chem Biol Interact, 242, pp.71~80 (2015)
- Han CH, "Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in D-galactose-induced BALB", c mice, 2014, pp.65, 356~363
- Maria Teresa Bertoldo Pacheco, "Effect of Different Hydrolysates of Whey Protein on Hepatic Glutathione Content in Mice", Journal of Medicinal Food, 8(3), pp.337~342 (2005), 한국식품영양과학회
- Guicciardi ME, "Apoptosis and necrosis in the liver", Compr Physiol, 3, pp.977~1010 (2013)
- Rock KL, "The inflammatory response to cell death. Annu Rev", Annu Rev Pathol: Mech Dis, 3, pp.99~126 (2008)