Enhancement of Lipid Metabolism and Antioxidant Defense Status in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder
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The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels,and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.
Abstract
The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels,and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.
참고문헌 (26)
- Aebi H, "Catalase, In Method of Enzymatic Analysis Vol. 2", pp.673~684 (1974), Academic Press
- Anderson JW, "Soluble fiber: hypocholesterolemic effects and proposed mechanisms" (1990)
- "Dietary Fiber: Chemistry, Physiology, and Health Effects", pp.339~363, Plenum Press
- Beutler E, "Red Cell Metabolism: A Manual of Biochemical Methods" (1971), Grune and Stratton
- Bray GA, "Dietary fat intake does affect obesity", Am J Clin Nutr, 68, pp.1157~1173 (1998)
- Fan JB, "Antioxidant activities of silk sericin from silkworm Bombyx mori", J Food Biochem, 33, pp.74~88 (2009)
- Folch JM, "A sample method for the isolation and purification of total lipids from animal tissue", J Biol Chem, 26, pp.497~509 (1957)
- Formiguera X, "Obesity: epidemiology and clinical aspects", Best Pract Res Clin Gastroenterol, 18, pp.1125~1146 (2004)
- Gallou-Kabani C, "C57BL", 6J and A, Obesity, pp.2007, 15[DOI]
- Hardy JG, "Polymeric materials based on silk proteins", Polymer, 49, pp.4309~4327 (2008)
- 홍성의, "실크 피브로인 산 가수분해물이 db", db mice의 지방세포 대사에 미치는 영향, 2002, pp.33(4), 312~318[DOI]
- Ibrahim W, "Oxidative stress and antioxidant status in mouse liver: effects of dietary lipid, vitamin E and iron", J Nutr, 127, pp.1401~1406 (1997)
- Kim MR, "Differential responses of hamsters and rats fed high-fat or low-fat diets supplemented with conjugated linoleic acid", Nutr Res, 22, pp.715~722 (2002)
- Kweon HY, "Characterization of tussah (Antheraea pernyi) silk fibroin powder prepared by HCl and NaOH", Korean J Seric Sci, 41, pp.54~60 (1999)
- Mackness MI, "Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein", FEBS Lett, 286, pp.152~154 (1991)
- Kweon HY, "Dissolution and characterization of regenerated Antheraea pernyi silk fibroin", J Appl Polym Sci, 82, pp.750~758 (2001)
- Marklund S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.469~474 (1974)
- Mize CE, "Hepatic glutathione reductase, purification and general kinetic properties", J Biol Chem, 237, pp.1589~1595 (1952)
- Mondal M, "The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn., - a review", Caspian J Env Sci, 5, pp.63~76 (2007)
- Ochoa S, "Malic dehydrogenase from pig heart, In Methods in Enzymology Vol. 1", pp.735~739 (1955), Academic Press
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Paglia ED, "Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase", J Lab Clin Med, 70, pp.158~169 (1967)
- Park J, "Overexpression of glucose-6-phosphate dehydrogenase is associated with lipid dysregulation and insulin resistance in obesity", MOLECULAR AND CELLULAR BIOLOGY, 25, pp.5146~5157, AMER SOC MICROBIOLOGY
- KangSunRyu, "Effects and Mechanisms of Silkworm Powder as a Blood Glucose-Lowering Agent", International Journal of Industrial Entomology, 4(2), pp.93~100 (2002), 한국잠사학회
- Zhou J, "Safety evaluation of protein of silkworm (Antheraea pernyi) pupae", Food Chem Toxicol, 44, pp.1123~1130 (2006)
- Zuliani G, "The anti-atherogenic properties of HDL particles", Int Congr Ser, 1303, pp.103~110 (2007)