Schisandra Chinensis Baillon regulates the gene expression of phase II antioxidant/detoxifying enzymes in hepatic damage induced rats
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BACKGROUND/OBJECTIVES: This study investigated the antioxidant activities and hepatoprotective effects of Schisandra chinensisBaillon extract (SCE) against tert-butyl hydroperoxide (t-BHP)-induced oxidative hepatic damage in rats. MATERIALS/METHODS: Sprague-Dawley (SD) rats were pretreated with SCE (300, 600, and 1,200 mg/kg BW) or saline oncedaily for 14 consecutive days. On day 14, each animal, except those belonging to the normal control group, were injectedwith t-BHP (0.8 mmol/kg BW/i.p.), and all of the rats were sacrificed 16 h after t-BHP injection. RESULTS: Although no significant differences in AST and ALT levels were observed among the TC and SCE groups, the high-doseSCE group showed a decreasing tendency compared to the TC group. However, erythrocyte SOD activity showed a significantincrease in the low-dose SCE group compared with the TC group. On the other hand, no significant differences in hepatictotal glutathione (GSH) level, glutathione reductase (GR), and glutathione peroxidase (GSH-Px) activities were observed amongthe TC and SCE groups. Hepatic histopathological evaluation revealed that pretreatment with SCE resulted in reduced t-BHP-inducedincidence of lesions, such as neutrophil infiltration, swelling of liver cells, and necrosis. In particular, treatment with a highdose of SCE resulted in induction of phase II antioxidant/detoxifying enzyme expression, such as glutathione S-transferase(GST) and glutamate-cysteine ligase catalytic subunit (GCLC). CONCLUSIONS: Based on these results, we conclude that SCE exerts protective effects against t-BHP induced oxidative hepaticdamage through the reduction of neutrophil infiltration, swelling of liver cells, and necrosis. In addition, SCE regulates thegene expression of phase II antioxidant/detoxifying enzymes independent of hepatic antioxidant enzyme activity.
Abstract
BACKGROUND/OBJECTIVES: This study investigated the antioxidant activities and hepatoprotective effects of Schisandra chinensisBaillon extract (SCE) against tert-butyl hydroperoxide (t-BHP)-induced oxidative hepatic damage in rats. MATERIALS/METHODS: Sprague-Dawley (SD) rats were pretreated with SCE (300, 600, and 1,200 mg/kg BW) or saline oncedaily for 14 consecutive days. On day 14, each animal, except those belonging to the normal control group, were injectedwith t-BHP (0.8 mmol/kg BW/i.p.), and all of the rats were sacrificed 16 h after t-BHP injection. RESULTS: Although no significant differences in AST and ALT levels were observed among the TC and SCE groups, the high-doseSCE group showed a decreasing tendency compared to the TC group. However, erythrocyte SOD activity showed a significantincrease in the low-dose SCE group compared with the TC group. On the other hand, no significant differences in hepatictotal glutathione (GSH) level, glutathione reductase (GR), and glutathione peroxidase (GSH-Px) activities were observed amongthe TC and SCE groups. Hepatic histopathological evaluation revealed that pretreatment with SCE resulted in reduced t-BHP-inducedincidence of lesions, such as neutrophil infiltration, swelling of liver cells, and necrosis. In particular, treatment with a highdose of SCE resulted in induction of phase II antioxidant/detoxifying enzyme expression, such as glutathione S-transferase(GST) and glutamate-cysteine ligase catalytic subunit (GCLC). CONCLUSIONS: Based on these results, we conclude that SCE exerts protective effects against t-BHP induced oxidative hepaticdamage through the reduction of neutrophil infiltration, swelling of liver cells, and necrosis. In addition, SCE regulates thegene expression of phase II antioxidant/detoxifying enzymes independent of hepatic antioxidant enzyme activity.
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