상업용 효소를 이용한 인삼잎 유래 ginsenoside로부터 Compound K 생산
Production of Compound K using ginsenosides from ginseng leaf by commercial enzyme
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초록
본 연구에서는 상업용 효소를 이용하여 인삼잎 유래 ginsenoside로부터 Compound K를 생산하는 연구를 수행하였다. 인삼잎 추출물에 함유되어 있는 총 ginsenoside는 146.9 mg/g 이었으며 PPD-type ginsenoside인 Rb1, Rb2, Rc, Rd, 그리고, F2의 함양은 각각 5.7, 11.58, 19.8, 16.4, 그리고 9.8 mg/g 이었다. 상업용 효소인 Aspergillus niger 유래 pectinase, Cytolase PCL5는 인삼잎 추출물 유래 PPD-type ginsenoside로부터 Compound K 생산에 있어서 가장 높은 효소 활성을 보였다. 본 효소는 pH 4.5와 65℃ 조건에서 가장 높은 효소 활성을 나타내었으며, 50, 55, 60, 65, 70, 그리고 75℃ 에서 half-life는 각각 118, 94, 87, 60, 47, 그리고 8시간을 나타내는 온도안정성을 보였다. Compound K 생산에 있어서 PPD-type ginsenoside와 효소의 최적 농도는 각각 10 mg/g 과 100 U/mL를 나타내었다. 본 연구에서 확립된 최적조건에서 Cytolase PCL5는 PPD type ginsenoside로부터 Compound K를 몰농도 97% 전환하였으며, 10시간 동안 1 mole/L/h의 생산성으로 Compound K를 생산하였다. 이러한 결과는 인삼잎은 Compound K를 생산하는데 효율적으로 활용 될 수 있는 소재이며, 상업용 효소 Cytolase PCL5는 Compound K 생산에 있어서 유용한 효소라는 것을 시사하였다.
Abstract
This study aimed to prepare a valuable minor ginsenoside Compound K, using ginsenosides from ginseng leaf, by-products of ginseng, with a food-grade commercial enzyme Cytolase PCL 5. The total content of major ginsenosides in ginseng leaf extract was 146.9 mg/g and that of the PPD-type ginsenosides such as Rb1, Rb2, Rc, Rd and F2 was 5.7, 11.5, 19.8, 16.4, and 9.8 mg/g, respectively. Cytolase PCL5 showed very efficient enzymatic activity to produce Compound K from the PPD-type gisenosides of ginseng leaf extract. The Cytolase PCL5 exhibited the highest activity at pH 4.5 and 65℃, and the half-lives of the enzyme at 50, 55, 60, 65, 70, and 75℃ were 118, 94, 87, 60, 47, and 8 h, respectively. During Compound K production, the optimal concentrations of PPD-type ginsenosides and enzyme were 10 mg/g and 100 U/ml, respectively. Under optimum conditions, Cytolase PCL5 produced Compound K from PPD-type ginsenosides with a molar yield of over 97% and productivity of 1 mmole/L/h within 10 h. These results suggest that the ginseng leaf is a potential producer of Compound K and Cytolase PCL5 is a very useful enzyme for Compound K production.
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