국내 소비 수산자원 및 가공품 중의 비타민 A 및 E vitamers 분석 및 검증
Analysis of A and E vitamer profiles in domestic raw and processed seafood consumed in Korea
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Simple simultaneous alkaline extraction (saponification) coupled with HPLC was validated and applied for the analysis of ten vitamers of A (retinol and β-carotene) and E (α-, β-, γ-, and δ-tocopherols (T) and corresponding tocotrienols (T3)) in seafood. Limits of detection for retinol, β-carotene and α-T were 0.380 μg/100 g, 0.602 μg/100 g and 0.0002 mg/100 g, respectively. Analysis precision was excellent for all vitamers (RSD: repeatability <2.4%, reproducibility <4.5%). Recoveries of vitamers ranged from 92% to 110%, showing good accuracy. Total vitamin A and E activities varied among seafood types, with ranges of 0.00-311.77 μg retinol activity equivalent/100 g and 0.01-0.62 mg α-tocopherol equivalent/100 g, respectively. β-Carotene was found in some seafoods such as shellfish (ark shell, blue mussel and spiny top shell) and crustacean (gazami crab) samples as well as marine algae due to the presence of intestinal organ containing algae in edible parts. The highest β-carotene content (689.84±29.86 μg/100 g) was observed in seasoned oyster whereas the retinol content was highest in blackthroat seaperch (311.77±7.99 μg/100 g). α-T was found in all samples and ranged from 0.07 to 4.72 mg/100 g. This study provides reliable vitamin A and E profiles for domestic seafood in Korea.
Abstract
Simple simultaneous alkaline extraction (saponification) coupled with HPLC was validated and applied for the analysis of ten vitamers of A (retinol and β-carotene) and E (α-, β-, γ-, and δ-tocopherols (T) and corresponding tocotrienols (T3)) in seafood. Limits of detection for retinol, β-carotene and α-T were 0.380 μg/100 g, 0.602 μg/100 g and 0.0002 mg/100 g, respectively. Analysis precision was excellent for all vitamers (RSD: repeatability <2.4%, reproducibility <4.5%). Recoveries of vitamers ranged from 92% to 110%, showing good accuracy. Total vitamin A and E activities varied among seafood types, with ranges of 0.00-311.77 μg retinol activity equivalent/100 g and 0.01-0.62 mg α-tocopherol equivalent/100 g, respectively. β-Carotene was found in some seafoods such as shellfish (ark shell, blue mussel and spiny top shell) and crustacean (gazami crab) samples as well as marine algae due to the presence of intestinal organ containing algae in edible parts. The highest β-carotene content (689.84±29.86 μg/100 g) was observed in seasoned oyster whereas the retinol content was highest in blackthroat seaperch (311.77±7.99 μg/100 g). α-T was found in all samples and ranged from 0.07 to 4.72 mg/100 g. This study provides reliable vitamin A and E profiles for domestic seafood in Korea.
참고문헌 (26)
- AOAC, "AOAC Guidelines for Single Labrtatory Validation of Chemical Methods for Dietary Supplements and Baotanicals", Association of Official Analytical Chemists, 12~13 (2002)
- AOAC, "AOAC Guidelines for Single Labrtatory Validation of Chemical Methods for Dietary Supplements and Baotanicals", Association of Official Analytical Chemists, 22~ (2002)
- 김영길, "국내 시판 유아용 고형 조제분유의 비타민 함량에 관한 조사", 생명과학회지, 17(12), pp.1689~1694 (2007), 한국생명과학회
- Ball GFM, "Vitamins in Foods-Analysis, Bioavailability and Stability", pp.3~ (2005), Taylor & Francis
- 천아름, "미강의 토코페롤 및 토코트리에놀 함량과 항산화 물질의 품종간 비교", 한국작물학회지, 58(4), pp.367~375 (2013), 한국작물학회[DOI]
- Dominguez R, "Simple and rapid method for the sinultaneous determination of cholesterol and retinol in meat using normal-phase HPLC technique", Food Anal Methods, 11, pp.319~326 (2018)
- Dror DK, "Vitamin E deficiency in developing countries", Food Nutr Bull, 32, pp.124~143 (2011)
- Gornas P, "An alternatice RP-HPLC method for the separation and determination of tocopherol and tocotrienol homologues as butter authenticity markers : A comparative study between two European countries", Eur J Lipid Sci Technol, 116, pp.895~903 (2014)
- 한영희, "국내에서 판매되는 임산부용 비타민·무기질 보충제에 함유된영양소의 종류와 함량 및 적절성 평가", 대한지역사회영양학회지, 23(4), pp.341~351 (2018), 대한지역사회영양학회[DOI]
- Hwang GJ, "Elderly people and vitamins", J Kor Soc HealthSyst Pharm, 19, pp.147~152 (2002)
- 황경미, "HPLC를 이용한 조제유류 중 베타카로틴 함량 분석 연구", 한국식품위생안전성학회지, 34(4), pp.334~339 (2019), 한국식품위생안전성학회[DOI]
- Karnjanqwipagul P, "Analysis of β-carotene in carrot by spectrophotometry", Mahidol University J Pharm Sci, 37, pp.8~16 (2010)
- 김동섭, "저장 온도 및 상대습도가 비타민 정제 중 지용성 비타민 함량의변화에 미치는 영향", Applied Biological Chemistry, 49(2), pp.103~107 (2006), 한국응용생명화학회
- 김효진, "국가표준식품성분표 개정을 위한 국내 다소비 해조류 및 채소류의 비타민 E 및 K 분석", 한국식품과학회지, 53(1), pp.19~28 (2021), 한국식품과학회
- Korea Food and Drug Administration (KFDA), "Korean Food Standard Codex", Korea Food Drug Administration, 131~137 (2017)
- 이계임 Lee Kyei-Im, "수산물 소비구조 분석", 농촌경제, 26(3), pp.21~38 (2003), 한국농촌경제연구원
- Ministry of Health and Welfare, "Dietary Reference Intakes for Koreans", 11~14 (2020)
- Ministry of Health and Welfare, "Dietary Reference Intakes for Koreans", 92~93 (2020)
- 목종수, "한국 연안산 수산물의 일반성분 조성 및 영양평가", 한국수산과학회지, 40(5), pp.259~268 (2007), 한국수산과학회
- 천지연, "한국 식단의 찜류 및 조림류의 지용성 비타민 및 콜레스테롤 함량 분석 및 검증", 한국식품저장유통학회지, 26(7), pp.796~807 (2019), 한국식품저장유통학회
- National Institute of Fishieries Science (NIFS), "Composition Table Marine Products in Korea. 8th ed", National Institute of Fisheries Science, 1~394 (2018)
- 박선혜, "한국 지역특화음식의 콜레스테롤, 레티놀, 베타카로틴 및 비타민 E 분석", 한국식품영양과학회지, 47(4), pp.429~439 (2018), 한국식품영양과학회
- Semba RD, "Nutrition and Immune Function", pp.151~169 (2002), CABI
- Sommer A, "Vitamin A deficiency and clinical deisease : An historical overview", J Nutr, 138, pp.1835~1839 (2008)
- Tang G, "Bioconversion of dietary provitamin A carotenoids to vitamin A in humans", Am J Clin Nutr, 73, pp.1468~1473 (2010)
- Thomas JB, "Preparation and value assingment of standard reference material 968c fat-soluble citamins, carotenoids and cholesterol in human serum", Clin Chin Acta, 305, pp.141~155 (2001)