Immunoaffinity-HPLC/PDA를 이용한 국내 수산자원 및 제품의 미량비타민 B7(비오틴) 및 B12(코발라민류) 분석 및 검증
Validation of immunoaffinity-HPLC/PDA method for microvitamin (biotin and cobalamins) analysis in marine resources and products
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초록
Given that Korea is surrounded by water on three sides, fishery resources are abundant and consumed nationwide. To assess the dietary intake of micronutrients, reliable analysis should be performed. However, there is no verified method for the analysis of vitamins B7 and B12 in fishery resources. In this study, the immunoaffinity-high performance liquied chromatography (HPLC)/photodiode array-detector (PDA) was validated and applied to analyze microvitamins B7 (biotin) and B12 (cobalamins) in fishery producus. The specificity of the immunoaffinity-HPLC/PDA method was good for biotin and cyanocobalamin. The analytical quantitation limits for biotin and cyanocobalamin were 0.028 and 0.014 μg/100 g, respectively. The recovery of vitamins B7 and B12 ranged from 99.0% to 101.3%, indicating a good accuracy. The repeatability (CV 3.6%-3.8%) and reproducibility (CV 4.9%-6.1%) of immunoaffinity- HPLC/PDA assay for vitamins B7 and B12 were acceptable, indicating good precision. Vitamins B7 (1.06 to 10.55 μg/100 g) and B12 (0.36 to 36.13 μg/100 g) were detected in all fishery products (n=28) including 4 fish, 3 crustaceans, 3 marine algae, 3 cephalopods, 4 shellfish, 3 others and 8 products. The highest vitamin B7 and B12 contents were found in gazami crabs and ark shells, respectively. In conclusion, our data could be used for establishing national vitamin database on Korean fishery resources.
Abstract
Given that Korea is surrounded by water on three sides, fishery resources are abundant and consumed nationwide. To assess the dietary intake of micronutrients, reliable analysis should be performed. However, there is no verified method for the analysis of vitamins B7 and B12 in fishery resources. In this study, the immunoaffinity-high performance liquied chromatography (HPLC)/photodiode array-detector (PDA) was validated and applied to analyze microvitamins B7 (biotin) and B12 (cobalamins) in fishery producus. The specificity of the immunoaffinity-HPLC/PDA method was good for biotin and cyanocobalamin. The analytical quantitation limits for biotin and cyanocobalamin were 0.028 and 0.014 μg/100 g, respectively. The recovery of vitamins B7 and B12 ranged from 99.0% to 101.3%, indicating a good accuracy. The repeatability (CV 3.6%-3.8%) and reproducibility (CV 4.9%-6.1%) of immunoaffinity- HPLC/PDA assay for vitamins B7 and B12 were acceptable, indicating good precision. Vitamins B7 (1.06 to 10.55 μg/100 g) and B12 (0.36 to 36.13 μg/100 g) were detected in all fishery products (n=28) including 4 fish, 3 crustaceans, 3 marine algae, 3 cephalopods, 4 shellfish, 3 others and 8 products. The highest vitamin B7 and B12 contents were found in gazami crabs and ark shells, respectively. In conclusion, our data could be used for establishing national vitamin database on Korean fishery resources.
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