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Physicochemical properties and physiological activity of bean sprouts extract containing Hovenia dulcis Thunb concentrates

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초록

Reactive oxygen species are the byproducts of metabolic processes in the body, However, excessive amount may cause side effects such as cancer. Therefore, to reduce the production of these species, but their long-term administration at high doses may induce side effects. Hence, natural materials with antioxidant activities are attracting attention. Two of these natural materials are soybean sprouts and Hovenia dulcis Thunb. fruits, but few studies have evaluated the effects of their combination. Thus, we prepared a soybean sprout extract containing 1.5% H. dulcis Thunb. fruit concentrate (BHM) to develop a functional food material derived from natural products and then confirmed its physicochemical properties and physiological activity. Among the organic acids detected in BHM, malic acid exhibited the highest content of 1,451.03 ppm, and the main free sugars were glucose (645.48 ppm) and fructose (738.11 ppm). Taurine was the most abundant free amino acid at a concentration of 11.95 ppm, followed by those of arginine (10.97 ppm) and glutamic acid (10.16 ppm). Analyses of the mineral components revealed large amounts of Zn and Fe in BHM, and the respective total polyphenol and flavonoid contents in BHM were 957.16 and 601.93 ppm. The DPPH radical and H2O2 scavenging activities and reducing power indicated excellent antioxidant efficacy compared to the positive controls. Furthermore, blood alcohol and acetaldehyde concentrations were measured to confirm the hangover-relieving effects of BHM, with both significantly decreased (p<0.05). BHM displays potential for development as a functional food, and the results of this study may be used as basic data in further research.

Abstract

Reactive oxygen species are the byproducts of metabolic processes in the body, However, excessive amount may cause side effects such as cancer. Therefore, to reduce the production of these species, but their long-term administration at high doses may induce side effects. Hence, natural materials with antioxidant activities are attracting attention. Two of these natural materials are soybean sprouts and Hovenia dulcis Thunb. fruits, but few studies have evaluated the effects of their combination. Thus, we prepared a soybean sprout extract containing 1.5% H. dulcis Thunb. fruit concentrate (BHM) to develop a functional food material derived from natural products and then confirmed its physicochemical properties and physiological activity. Among the organic acids detected in BHM, malic acid exhibited the highest content of 1,451.03 ppm, and the main free sugars were glucose (645.48 ppm) and fructose (738.11 ppm). Taurine was the most abundant free amino acid at a concentration of 11.95 ppm, followed by those of arginine (10.97 ppm) and glutamic acid (10.16 ppm). Analyses of the mineral components revealed large amounts of Zn and Fe in BHM, and the respective total polyphenol and flavonoid contents in BHM were 957.16 and 601.93 ppm. The DPPH radical and H2O2 scavenging activities and reducing power indicated excellent antioxidant efficacy compared to the positive controls. Furthermore, blood alcohol and acetaldehyde concentrations were measured to confirm the hangover-relieving effects of BHM, with both significantly decreased (p<0.05). BHM displays potential for development as a functional food, and the results of this study may be used as basic data in further research.

참고문헌 (59)

  1. Abdel-Hameed ESS, "Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples", Food Chem, 114, pp.1271~1277 (2009)
  2. Benzi G, "Age-and peroxidative stressrelated modifications of the cerebral enzymatic activities linked to mitochondria and the glutathione system", Free Radic Biol Med, 19, pp.77~101 (1995)
  3. Blois MS, "Antioxidant activity determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  4. Cha PH, "Hovenia dulcis Thunb extract and its ingredient methyl vanillate activate Wnt", β-catenin pathway and increase bone mass in growing or ovariectomized mice, 2014, pp.9, e85546~
  5. 조영제, "구기자(Lycii fructus) 추출물의 항산화와 항고혈압 효과", 한국식품영양과학회지, 34(9), pp.1308~1313 (2005), 한국식품영양과학회
  6. 최지영, "헛개열매추출액발효물이 흰쥐의 에탄올 경구투여에 의한 간손상 지표와체중 감량 완화에 미치는 영향", Food Science and Preservation, 21(3), pp.412~420 (2014), 한국식품저장유통학회
  7. Choi YS, "Concentration of phytoestrogens in soybeans and soybean products in Korea", J Sci Food Agric, 80, pp.1709~1712 (2000)
  8. Collins JL, "Changes in trypsin inhibitory activity in some soybean varieties during maturation and germination", J Food Sci, 41, pp.168~172 (1976)
  9. Fiers W, "More than one way to die : Apoptosis and necrosis and reactive oxygen damage", Oncogene, 18, pp.7719~7730 (1999)
  10. Fukuzawa K, "Antioxidants", J Act Oxyg Free Rad, 1, pp.55~70 (1990)
  11. Gao X, "Evaluation of antioxidant activities of rosehip ethanol extracts in different test systems", J Sci Food Agric, 80, pp.2021~2027 (2000)
  12. Halliwell B, "Free radicals, antioxidants, and human disease: Where are we now?", J Lab Clin Med, 119, pp.598~620 (1992)
  13. 홍성준, "볶음처리에 따른 콩나물과 땅콩 새싹 추출물의 이화학적 특성 및 관능적 특성 변화", 한국식품영양과학회지, 49(4), pp.356~369 (2020), 한국식품영양과학회[DOI]
  14. 임도연, "열처리 조건에 따른 우엉차의 항산화 활성과 아미노산 함량의 변화", 생명과학회지, 29(12), pp.1358~1363 (2019), 한국생명과학회[DOI]
  15. Jeong CH, "Some functional properties of extracts from leaf and fruit stalk of Hovenia dulcis", Korean J Postharvest Sci Technol, 7, pp.291~296 (2000)
  16. Ji Y, "Effects of fruits of Hovenia dulcis Thunb on acute alcohol toxicity in mice", Zhong Yao Cai, 24, pp.126~128 (2001)
  17. Joo OS, "Changes in organic acid contents during drying and storage of shellfish meat", J Korean Soc Food Sci Nutr, 25(5), pp.774~778 (1996)
  18. 강명화, "각 부위별 활나물(Crotalaria sessiflora L.) 에탄올 추출물의 항산화효과", 한국식품과학회지, 34(6), pp.1098~1102 (2002), 한국식품과학회
  19. 강미정, "민들레 물추출물의 항산화 및 자유라디칼 소거활성", Food Science and Preservation, 9(2), pp.253~259 (2002), 한국식품저장유통학회
  20. 김현덕, "오미자 첨가량에 따른 Demi-Glace 소스의 일반성분과 유리당 함량 및 관능적 특성", 동아시아식생활학회지, 14(6), pp.598~607 (2004), 동아시아식생활학회
  21. 김현룡, "방울토마토 라이코펜 품종의 유리아미노산 및 폴리페놀 화합물의 분석", Culinary Science & Hospitality Research, 20(3), pp.37~49 (2014), (사)한국조리학회[DOI]
  22. 김홍태, "Carbon Tetrachloride로 유발된 Rat의 간장과 신장 손상시 헛개나무 추출액의 치료효과", 한국임상수의학회지, 28(1), pp.20~27 (2011), 한국임상수의학회
  23. 김정인, "Streptozotocin 유발 당뇨쥐에 있어서 콩나물 메탄올 추출물의 혈당강하효과", 한국식품영양과학회지, 32(6), pp.921~925 (2003), 한국식품영양과학회
  24. Kim MH, "Hepatic detoxification activity and reduction of serum alcohol concentration of Hovenia dulcis THUNB from Korea and China", Korean J Medicinal Crop Sci, 8, pp.225~233 (2000)
  25. Kim SD, "Composition of soybean sprouts and its nutritional value", Korea Soybean Dig, 10, pp.1~9 (1993)
  26. 김수지, "대추 열수 추출물의 항산화 및 영양학적 특성에 대한 Lactiplantibacillus plantarum 발효의 영향", Current Topics in Lactic Acid Bacteria and Probiotics, 7(2), pp.37~44 (2021), 한국유산균프로바이오틱스학회
  27. 김연희, "66품종 나물콩과 콩나물의 isoflavone분석", 한국식품과학회지, 35(4), pp.568~575 (2003), 한국식품과학회
  28. Ko EJ, "The estavlishment of optimum cultural conditions for manufacturing garlic vinegar", J Korean Soc Food Sci Nutr, 27, pp.102~108 (1998)
  29. 이현정, "백국과 황국 추출물의 항산화활성 및 항유전독성 효과", 한국식품영양과학회지, 41(3), pp.289~294 (2012), 한국식품영양과학회
  30. 이재민, "DPPH법에 의한 식용유지의 열산화 안정성 비교", 한국식품과학회지, 39(2), pp.133~137 (2007), 한국식품과학회
  31. 신승렬, "능이버섯의 영양성분에 관한 연구", Food Science and Preservation, 10(1), pp.65~69 (2003), 한국식품저장유통학회
  32. 이숙영, "동백나무 잎과 꽃 추출물의 항미생물 활성 및 항산화 효과", 한국약용작물학회지, 13(3), pp.93~100 (2005), 한국약용작물학회
  33. 이소연, "瓊玉膏의 성분 분석 및 추출물별 抗酸化 효능 비교", 대한본초학회지, 23(2), pp.123~136 (2008), 대한본초학회
  34. 이양숙, "우산나물(Syneilesis palmata) 추출물의 항산화 활성", 한국식품영양과학회지, 38(11), pp.1471~1477 (2009), 한국식품영양과학회
  35. 이영숙, "계피 추출물의 항산화 효과 및 화장품소재의 응용", 아시안뷰티화장품학술지, 17(1), pp.69~80 (2019), 한국피부과학연구원[DOI]
  36. 임미경, "헛개나무추출액 및 생약성분의 혼용이 사염화탄소 투여로 유발된 흰쥐의 급성 간 및 신장손상의 회복에 미치는 영향", 한국임상수의학회지, 24(3), pp.400~405 (2007), 한국임상수의학회
  37. 임선미, "와송(Orostachys japonicus) 추출물의 항산화 및 생리 활성", Journal of Applied Biological Chemistry, 60(4), pp.293~300 (2017), 한국응용생명화학회
  38. Masaki H, "Activeoxygen scavenging activity of plant extracts", Biol Pharm Bull, 18, pp.162~166 (1995)
  39. Muller HE, "Detection of hydrogen peroxide produced by microorganisms on an ABTS peroxidase medium", Zbl Bakt Hyg A, 259, pp.151~154 (1985)
  40. 나천수, "랫드를 이용한 헛개나무과병 열수추출물 투여가 운동에 의한피로회복과 운동수행능력에 미치는 기전 규명", 약 학 회 지, 57(5), pp.348~356 (2013), 대한약학회
  41. 남승우, "미숙과 건여주(Momordica charantia L.) 추출물의 항산화 활성 및 암세포 증식 억제 효과", 한국식품영양과학회지, 46(12), pp.1453~1461 (2017), 한국식품영양과학회
  42. Oh SL, "Composition of free sugars, free amino acids, non-volatile organic acids and tannins in the extracts of L. chinensis M., A. autiloba K., S. chinensis B. and A. sessiliflorum S", Korean J Food Sci Technol, 22, pp.76~81 (1990)
  43. 옥둘이, "홍합과 여러 식물의 열수 추출물의 알코올분해효소에 미치는 영향과 항산화 및 ACE 저해 활성", 한국식품조리과학회지, 30(5), pp.613~619 (2014), 한국식품조리과학회
  44. 박금순, "헛개나무의 부위별 이화학적 및 관능적 품질 특성", 동아시아식생활학회지, 15(1), pp.65~70 (2005), 동아시아식생활학회
  45. Park JS, "HPLC determination of bioactive flavonoids in Hovenia dulcis fruit extracts", J Chromatographic Sci, 54, pp.130~135 (2015)
  46. Park SC, "Effect of bean sprout extracts on metabolism and biological functions of ethanol in vitro and in vivo", Korea Soybean Dig, 10, pp.123~143 (1994)
  47. 임종환, "대두의 수화속도와 콩나물의 발아특성에 대한 동결처리 효과", 한국식품과학회지, 35(4), pp.660~665 (2003), 한국식품과학회
  48. 임태진, "개머루덩굴 추출물의 항산화 효과", 한국자원식물학회지, 23(5), pp.445~450 (2010), 한국자원식물학회
  49. Shatoh R, "ACTCAT, a novel cisacting element for proline- and hypoosmolarityresponsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis", Plant Physiol, 130, pp.709~719 (2002)
  50. Shiddhuraju P, "Studies on the antioxidant activity of Indian laburnum(Cassia fistula L. ) : A reliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp", Food Chem, 79, pp.61~67 (2002)
  51. Shin TS, "Effects of mixed natural antioxidants on POV and TBARS of cooked ground pork", J Agri Tech Dev Inst, 3, pp.1~6 (1999)
  52. 손희경, "시판 콩나물로 제조한 콩나물 국의 품질 특성", 한국생활과학회지, 18(5), pp.1147~1153 (2009), 한국생활과학회
  53. Shumate RP, "A study of the metabolism rates of alcohol in the human body", J Forensic Med, 14, pp.83~100 (1967)
  54. 성혜미, "고농도 산소수로 재배한 콩나물 추출 음료의 알코올 분해 효능", 한국식품과학회지, 46(5), pp.616~621 (2014), 한국식품과학회[DOI]
  55. Van HP, "Phenolic compounds of cereals and their antioxidant capacity", Crit Rev Food Sci Nutr, 56, pp.25~35 (2016)
  56. 우승미, "정치배양 및 시판 현미식초의 품질특성 비교", Food Science and Preservation, 19(2), pp.301~307 (2012), 한국식품저장유통학회[DOI]
  57. Yen GC, "Antioxidant activity of extracts from Du-zhong(Eucommia ulmoides)toward various lipid peroxidation models in vitro", J Agric Food Chem, 46, pp.3952~3957 (1998)
  58. Yildirim A, "Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts", J Agric Food Chem, 49, pp.4083~4089 (2001)
  59. 유양희, "세포 및 동물모델에서의 알코올에 의해 유발된 간손상에 대한 지구자 추출물의 보호효과", 한국식품영양과학회지, 38(2), pp.154~159 (2009), 한국식품영양과학회

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