Antimicrobial, Antioxidant and Cytotoxic Activities of Dendropanax morbifera Léveille extract for mouthwash and denture cleaning solution
1
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
PURPOSE. The purpose of this study was to analyze the antimicrobial, antioxidant activity and cytotoxicity of Dendropanax morbifera Léveille extract for assessing whether Dendropanax morbifera Léveille can be used for the development of natural mouthwash and denture cleaning solution. MATERIALS AND METHODS. The extract was obtained from branches of Dendropanax morbifera Léveille. The solvent fractions were acquired by fractionating Dendropanax morbifera Léveille extract using n-hexane, ethyl acetate, chloroform and butanol solvent. Paper disc test was used to evaluate the antimicrobial and antifungal activity of Dendropanax morbifera Léveille extract and solvent fractions against Streptococcus mutans and Candida albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of Dendropanax morbifera Léveille extract was analyzed through MTT assay using normal human oral keratinocytes. RESULTS. Dendropanax morbifera Léveille extract showed antimicrobial activity against Streptococcus mutans and especially Candida albicans. The solvent fractions of Dendropanax morbifera Léveille showed strong antimicrobial activity against Streptococcus mutans and Candida albicans in n-hexane and butanol solvent fraction, respectively. Dendropanax morbifera Léveille extract also showed outstanding antioxidant activity. Butanol, ethyl acetate, and chloroform solvent fraction of Dendropanax morbifera Léveille tended to have increased antioxidant activity as the concentration increased. Dendropanax morbifera Léveille extract showed high cell survival rate in cytotoxicity test. CONCLUSION. Dendropanax morbifera Léveille extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that Dendropanax morbifera Léveille is applicable as an ingredient for natural mouthwash and denture cleanser.
Abstract
PURPOSE. The purpose of this study was to analyze the antimicrobial, antioxidant activity and cytotoxicity of Dendropanax morbifera Léveille extract for assessing whether Dendropanax morbifera Léveille can be used for the development of natural mouthwash and denture cleaning solution. MATERIALS AND METHODS. The extract was obtained from branches of Dendropanax morbifera Léveille. The solvent fractions were acquired by fractionating Dendropanax morbifera Léveille extract using n-hexane, ethyl acetate, chloroform and butanol solvent. Paper disc test was used to evaluate the antimicrobial and antifungal activity of Dendropanax morbifera Léveille extract and solvent fractions against Streptococcus mutans and Candida albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of Dendropanax morbifera Léveille extract was analyzed through MTT assay using normal human oral keratinocytes. RESULTS. Dendropanax morbifera Léveille extract showed antimicrobial activity against Streptococcus mutans and especially Candida albicans. The solvent fractions of Dendropanax morbifera Léveille showed strong antimicrobial activity against Streptococcus mutans and Candida albicans in n-hexane and butanol solvent fraction, respectively. Dendropanax morbifera Léveille extract also showed outstanding antioxidant activity. Butanol, ethyl acetate, and chloroform solvent fraction of Dendropanax morbifera Léveille tended to have increased antioxidant activity as the concentration increased. Dendropanax morbifera Léveille extract showed high cell survival rate in cytotoxicity test. CONCLUSION. Dendropanax morbifera Léveille extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that Dendropanax morbifera Léveille is applicable as an ingredient for natural mouthwash and denture cleanser.
참고문헌 (36)
- Glass RT, "Evaluation of microbial flora found in previously worn prostheses from the Northeast and Southwest regions of the United States", J Prosthet Dent, 103, pp.384~389 (2010)
- Budtz-Jörgensen E, "The significance of Candida albicans in denture stomatitis", Scand J Dent Res, 82, pp.151~190 (1974)
- Baena-Monroy T, "Candida albicans, Staphylococcus aureus and Streptococcus mutans colonization in patients wearing dental prosthesis", Med Oral Patol Oral Cir Bucal, 10, pp.E27~E39 (2015)
- Campos MS, "Biofilm microbial communities of denture stomatitis", Oral Microbiol Immunol, 23, pp.419~424 (2008)
- Ramage G, "Denture stomatitis: a role for Candida biofilms", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 98, pp.53~59 (2004)
- Caton JG, "Comparison between mechanical cleaning and an antimicrobial rinse for the treatment and prevention of interdental gingivitis", J Clin Periodontol, 20, pp.172~178 (1993)
- Newman DJ, "Natural products as sources of new drugs over the period 1981-2002", J Nat Prod, 66, pp.1022~1037 (2003)
- 이서호, "황칠나무 잎의 면역활성증진기능 탐색", 한국약용작물학회지, 10(2), pp.109~115 (2002), 한국약용작물학회
- Hyun TK, "Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Léveille", Food Chem, 141, pp.1947~1955 (2013)
- Moon HI, "Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats", Hum Exp Toxicol, 30, pp.870~875 (2011)
- Chung IM, "Antiatherogenic activity of Dendropanax morbifera essential oil in rats", Pharmazie, 64, pp.547~549 (2009)
- Chung IM, "Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille", Phytother Res, 25, pp.784~786 (2011)
- Lee CK, "Screening and isolation of antibiotic resistance inhibitors from herb materialsresistance inhibition of volatile components of Korean aromatic herbs", Arch Pharm Res, 21, pp.62~66 (1998)
- Park YK, "Current review on the study of antioxidants developments from forest resources", Trend Agric Life Sci, 4, pp.1~13 (2006)
- Wikins TD, "Standardized single-disc method for antibiotic susceptibility testing of anaerobic bacteria", Antimicrob Agents Chemother, 1, pp.451~459 (1972)
- Brand-Williams W, "Use of a free radical method to evaluate antioxidant activity", Technol, 28, pp.25~30 (1995)
- Pratten J, "Response of single species biofilms and microcosm dental plaques to pulsing with chlorhexidine", J Antimicrob Chemother, 42, pp.453~459 (1998)
- Gjermo P, "Chlorhexidine and related-compounds", J Dent Res, 68, pp.1602~1608 (1989)
- Kim H, "Analysis and recordings of orally transmitted knowledge about medicinal plants in the southern mountainous region of Korea", J Ethnopharmacol, 134, pp.676~696 (2011)
- Islam B, "Dental caries: from infection to prevention", Med Sci Monit, 13, pp.RA196~RA203 (2007)
- Kulak Y, "Existence of Candida albicans and microorganisms in denture stomatitis patients", J Oral Rehabil, 24, pp.788~790 (1997)
- Studies on the TechnicalImprovement to use “Hwangchil-traditional Korean golden varnish” and the diversified uses of Dendropanax morbiera Lev.. 2nd Report, "Im GP", Korean Ministry of Science and Technology, 1~257 (1996)
- 백동헌, "구강 질환 유발 미생물에 대한 항생작용을 갖는 천연물 추출물 검색", 미생물학회지, 43(3), pp.227~231 (2007), 한국미생물학회
- Otake S, "Anticaries effects of polyphenolic compounds from Japanese green tea", Caries Res, 25, pp.438~443 (1991)
- Smullen J, "The antibacterial activity of plant extracts containing polyphenols against Streptococcus mutans", Caries Res, 41, pp.342~349 (2007)
- Kim HR, "Chemical characteristics of the leaves and the seeds of Korean Dendropanax (Dendropanax morbifera Lev.).", J Korean Soc Agric Chem Biotechnol, 43, pp.63~66 (2000)
- Yanagida A, "Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutans streptococci", J Agric Food Chem, 48, pp.5666~5671 (2000)
- 손호용, "Candidiosis 치료제 개발을 위한 약용 및 야생 식물의 항진균 활성의 검색", 생명과학회지, 13(5), pp.604~617 (2003), 한국생명과학회
- Coleman JJ, "Characterization of plant-derived saponin natural products against Candida albicans", ACS Chem Biol, 5, pp.321~332 (2010)
- Zou Y, "Phenolics and antioxidant activity of mulberry leaves depend on cultivar and harvest month in Southern China", Int J Mol Sci, 13, pp.16544~16553 (2012)
- 홍슬기, "흰씀바귀 (苦菜, Ixeris dentata var. albiflora Nakai)뿌리의 성분 분석과 추출물의 세포 생존율 및 DPPH 라디칼 소거 활성", Journal of Nutrition and Health, 43(2), pp.105~113 (2010), 한국영양학회
- 사재훈, "생열귀나무의 카테킨 함량 및 항산화효과", 생약학회지, 33(3), pp.177~181 (2002), 한국생약학회
- 장주리, "도라지 부탄올 추출물의 항산화 및 nitric oxide 생성 저해 효과", 한국식품저장유통학회지, 18(1), pp.65~71 (2011), 한국식품저장유통학회
- Helgeland K, "Effect of chlorhexidine on animal cells in vitro", Scand J Dent Res, 79, pp.209~215 (1971)
- Carmichael J, "Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing", Cancer Res, 47, pp.936~942 (1987)
- 박수아, "황칠나무 잎 추출물의 세포 항산화 활성과 미백활성 측정", 한국미생물·생명공학회지, 41(4), pp.407~415 (2013), 한국미생물·생명공학회[DOI]