Molecular analysis of Anisakis type I larvae in marine fish from three different sea areas in Korea
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Anisakiasis, a human infection of Anisakis L3 larvae, is one of the common foodborne parasitic diseases in Korea. Studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. In this study, we analyzed the molecular characteristics of 174 Anisakis type I larvae collected from 10 species of fish caught in 3 different sea areas in Korea. PCR-RFLP and sequence analyses of rDNA ITS and mtDNA cox1 revealed that the larvae showed interesting distribution patterns depending on fish species and geographical locations. Anisakis pegreffii was predominant in fish from the Yellow Sea and the South Sea. Meanwhile, both A. pegreffii and A. simplex sensu stricto (A. simplex s.str.) larvae were identified in fish from the East Sea, depending on fish species infected. These results suggested that A. pegreffii was primarily distributed in a diverse species of fish in 3 sea areas around Korea, but A. simplex s.str. was dominantly identified in Oncorhynchus spp. in the East Sea.
Abstract
Anisakiasis, a human infection of Anisakis L3 larvae, is one of the common foodborne parasitic diseases in Korea. Studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. In this study, we analyzed the molecular characteristics of 174 Anisakis type I larvae collected from 10 species of fish caught in 3 different sea areas in Korea. PCR-RFLP and sequence analyses of rDNA ITS and mtDNA cox1 revealed that the larvae showed interesting distribution patterns depending on fish species and geographical locations. Anisakis pegreffii was predominant in fish from the Yellow Sea and the South Sea. Meanwhile, both A. pegreffii and A. simplex sensu stricto (A. simplex s.str.) larvae were identified in fish from the East Sea, depending on fish species infected. These results suggested that A. pegreffii was primarily distributed in a diverse species of fish in 3 sea areas around Korea, but A. simplex s.str. was dominantly identified in Oncorhynchus spp. in the East Sea.
참고문헌 (31)
- Chai JY, "Fish-borne parasitic zoonoses : status and issues", Int J Parasitol, 35, pp.1233~1254 (2005)
- Audicana MT, "Anisakis simplex : from obscure infectious worm to inducer of immune hypersensitivity", Clin Microbiol Rev, 21, pp.360~379 (2008)
- Hochberg NS, "Anisakidosis : perils of the deep", Clin Infect Dis, 51, pp.806~812 (2010)
- Sohn WM, "Oxford Textbook of Zoonoses-Biology, Clinical Practice, and Public Health Control", pp.774~786 (2011), Oxford Univer sity Press
- Seon Hee Choi, "Anisakis simplex Larvae: Infection Status in Marine Fish and Cephalopods Purchased from the Cooperative Fish Market in Busan, Korea", The Korean Journal of Parasitology, 49(1), pp.39~44 (2011), 대한기생충학ㆍ열대의학회
- Setyobudi E, "Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea", Parasitol Res, 108, pp.585~592 (2011)
- Shin-Hyeong Cho, "Larval Anisakid Infections in Marine Fish from Three Sea Areas of the Republic of Korea", The Korean Journal of Parasitology, 50(4), pp.295~299 (2012), 대한기생충학ㆍ열대의학회
- Eko Setyobudi, "Molecular Identification of Anisakid Nematodes Third Stage Larvae Isolated from Common Squid (Todarodes pacificus) in Korea", Ocean Science Journal, 48(2), pp.197~205 (2013), 한국해양과학기술원[DOI]
- D’Amelio S, "Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis(Nematoda : Ascaridoidea)defined by polymerase chain reaction-based restriction fragment length polymorphism", Int J Parasitol, 30, pp.223~226 (2000)
- Hu M, "Mutation scanning for sequence variation in three mitochondrial DNA regions for members of the Contracaecum osculatum(Nematoda : Ascaridoidea)complex", Electrophoresis, 22, pp.1069~1075 (2001)
- Umehara A, "Molecular identification of Anisakis simplex sensu stricto and Anisakis pegreffii(Nematoda : Anisakidae)from fish and cetacean in Japanese waters", Parasitol Int, 55, pp.267~271 (2006)
- Tamura K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0", Mol Biol Evol, 24, pp.1596~1599 (2007)
- Berland B, "Nematodes from some Norwegian marine fishes", Sarsia, 2, pp.1~50 (1961)
- Iglesias R, "Molecular and morphological evidence for the occurrence of Anisakis sp. A (Nematoda, Anisakidae) in Blainville’s beaked whale Mesoplodon densirotris", J Helminthol, 82, pp.305~308 (2008)
- Mattiucci S, "Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host–parasite co-evolutionary processes", Adv Parasitol, 66, pp.47~248 (2008)
- Arizono N, "Anisakis simplex sensu stricto and Anisakis pegreffii : biological characteristics and pathogenic potential in human anisakiasis", Foodborne Pathog Dis, 9, pp.1~5 (2012)
- Pontes T, "Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species", J Parasitol, 91, pp.1430~1434 (2005)
- Chen Q, "Specific PCR assays for the identification of common anisakid nematodes with zoonotic potential", Parasitol Res, 104, pp.79~84 (2008)
- Abe N, "Molecular characterization of Anisakis pegreffii larvae in pacific cod in Japan", J Helminthol, 79, pp.303~306 (2005)
- Umehara A, "Molecular identification of Anisakis type I larvae isolated from hairtail fish off the coasts of Taiwan and Japan", Int J Food Microbiol, 143, pp.161~165 (2010)
- Du C, "Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach", Electrophoresis, 31, pp.654~658 (2010)
- Quiazon KMA, "Experimental challenge of Anisakis simplex sensu stricto and Anisakis pegreffii(Nematoda : Anisakidae)in rainbow trout and olive flounder", Parasitol Int, 60, pp.126~131 (2011)
- Abollo E, "Occurrence of recombinant genotypes of Anisakis simplex s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry", Infect Genet Evol, 3, pp.175~181 (2003)
- Martin-Sanchez J, "Structure of Anisakis simplex s. l. populations in a region sympatric for A. pegreffii and A. simplex s. s. Absence of reproductive isolation between both species", Mol Biochem Parasitol, 141, pp.155~162 (2005)
- Umehara A, "Molecular identification of the etiological agent of the human anisakiasis in Japan", Parasitol Int, 56, pp.211~215 (2007)
- D’Amelio S, "Diagnosis of a case of gastric anisakidosis by PCR-based restriction fragment length polymorphism analysis", Parassitologia, 41, pp.591~593 (1999)
- Fumarola L, "Anisakis pegreffi etiological agent of gastric infections in two Italian women", Foodborne Pathog Dis, 6, pp.1157~1159 (2009)
- Suzuki J, "Risk factors for human Anisakis infection and association between the geographic origins of Scomber japonicus and anisakid nematodes", Int J Food Microbiol, 137, pp.88~93 (2010)
- 이은정, "상부 위장관 아니사키스증 141예에서 점막의 변화 및 영향인자", 대한소화기학회지, 53(2), pp.90~97 (2009), 대한소화기학회
- Kyong-Rock Do, "Intestinal Helminthic Infections Diagnosed by Colonoscopy in a Regional Hospital during 2001-2008", The Korean Journal of Parasitology, 48(1), pp.75~78 (2010), 대한기생충학ㆍ열대의학회
- 김태윤, "Comparison of the Clinical Characteristics of Patients with Small Bowel and Gastric Anisakiasis in Jeju Island", Gut and Liver, 7(1), pp.23~29 (2013), 거트앤리버 발행위원회