Nested-PCR and a new ELISA-based NovaLisa test kit for malaria diagnosis in an endemic area of Thailand
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.
Abstract
Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.
참고문헌 (17)
- Na-Bangchang K, "Declining in efficacy of a three-day combination regimen of mefloquine-artesunate in a multi-drug resistance area along the Thai-Myanmar border", Malar J, 9, pp.273~ (2010)
- Noppadon Tangpukdee, "Malaria Diagnosis: A Brief Review", The Korean Journal of Parasitology, 47(2), pp.93~102 (2009), 대한기생충학ㆍ열대의학회
- Yatsushiro S, "Rapid and highly sensitive detection of malaria-infected erythrocytes using a cell microarray chip", PLoS One, 5, pp.e13179~ (2010)
- She RC, "Comparison of immunofluorescence antibody testing and two enzyme immunoassays in the serologic diagnosis of malaria", J Travel Med, 14, pp.105~111 (2007)
- Reesink HW, "European strategies against the parasite transfusion risk", Transfus Clin Biol, 12, pp.1~4 (2005)
- Kamau E, "Development of a highly sensitive genus-specific quantitative reverse transcriptase real-time PCR assay for detection and quantitation of plasmodium by amplifying RNA and DNA of the 18S rRNA genes", J Clin Microbiol, 49, pp.2946~2953 (2011)
- "Annual epidemiological surveillance report 2011"[URL]
- Wooden J, "PCR and strain identification in Plasmodium falciparum", Parasitol Today, 9, pp.303~305 (1993)
- Singh B, "A genus-and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies", Am J Trop Med Hyg, 60, pp.687~692 (1999)
- Wanna Chaijaroenkul, "Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand", The Korean Journal of Parasitology, 49(1), pp.33~38 (2011), 대한기생충학ㆍ열대의학회
- Phimpraphi W, "Longitudinal study of Plasmodium falciparum and Plasmodium vivax in a Karen population in Thailand", Malar J, 7, pp.99~ (2008)
- Dinko B, "Persistent detection of Plasmodium falciparum, P. malariae, P. ovale curtisi and P. ovale wallikeri after ACT treatment of asymptomatic Ghanaian school-children", Int J Parasitol Drugs Drug Resist, 3, pp.45~50 (2013)
- Na-Bangchang K, "Current malaria status and distribution of drug resistance in East and Southeast Asia with special focus to Thailand", Tohoku J Exp Med, 211, pp.99~113 (2007)
- Doderer C, "A new ELISA kit which uses a combination of Plasmodium falciparum extract and recombinant Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies", Malar J, 6, pp.19~ (2007)
- Moges B, "Comparison of CareStart HRP2", pLDH COMBO rapid malaria test with light microscopy in north-west Ethiopia, 2012, pp.11, 234~
- Noedl H, "Sensitivity and specificity of an antigen detection ELISA for malaria diagnosis", Am J Trop Med Hyg, 75, pp.1205~1208 (2006)
- Choudhury N, "Malaria screening to prevent transmission by transfusion : an evaluation of techniques", Med Lab Sci, 4, pp.206~211 (1991)