시마연어, Oncorhynchus masou에서 분리된 아니사키스속 선충 3기 유충의 분자생물학적 방법을 이용한 동정
Molecular identification of Anisakid worm third stage larvae isolated from masou salmon Oncorhynchus masou
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon Oncorhynchus masou. Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.
Abstract
Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon Oncorhynchus masou. Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.
참고문헌 (24)
- Audicana, M. T., "Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity", Clin. Microbiol. Rev., 21(2), pp.360~379 (2008)
- Berland, B., "Nematode from some Norwegian marine fishes", Sarsia, 2, pp.1~50 (1961)
- Chai, J.Y., "Fish-borne parasitic zoonosis: Status and issues", Int. J. Parasitol., 35, pp.1233~1254 (2005)
- D’Amelio, S., "Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda; Ascaridoidea) defined by polymerase chain reaction-base restriction fragement length polymorphism", Int. J. Parasitol., 30, pp.223~226 (2000)
- D’Amelio, S., "Anisakis. in: Molecular detection of foodborne pathogens", pp.757~768 (2009), CRC Taylor and Francis Press
- Deardorff, T.L., "Prevalence of larval Anisakis simplex in pen-reared and wild-caught salmon (Salmonidae) from Puget Sound, Washington", J. Wildlife. Dis., 25, pp.416~419 (1989)
- Iglesias, R., "Antigenic cross-reactivity in mice between third-stage larvae of Anisakis simplex and other nematodes", Parasitol. Res., 82, pp.378~381 (1996)
- Felsenstein, J., "PHYLIP (Phylogeny Inference Package) version 3.67" (2007), Dept of Genetics, Univ of Washington
- 강주희, "고래회충 검출을 위한 육안검사법과 중합효소연쇄반응-제한효소절편길이다형성의 비교", 한국식품위생안전성학회지, 23(4), pp.314~318 (2008), 한국식품위생안전성학회
- Kim, K.H., "Morphological classification and infection rate of Anisakid larvae in marine fishes", Kor. J. Rural. Med., 13(1), pp.32~40 (1988)
- Kim, K.H., "A study about infection state of anisakid larvae and parasitic helminths in salmon (Oncorhynchus keta) and sea trout (Oncorhynchus masou) which caught from Taean port Kangwon Do", Kor. J. Rural Med., 15(2), pp.27~32 (1990)
- 김선미, "고래회충유충증 감별 진단을 위한 18S ribosomal DNA (rDNA) PCR-RFLP 법 적용", 생명과학회지, 19(9), pp.1328~1332 (2009), 한국생명과학회
- Nadler, S.A., "Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution", J. Parasitol., 86, pp.380~393 (2000)
- Nadler, S.A., "Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mamals", J. Parasitol., 91, pp.1413~1429 (2005)
- Smith, J.W., "Anisakis and anisakiasis", Adv. parasitol., 16, pp.93~163 (1978)
- Song, S.B., "Infection status of larval anisakids in Astroconger myriaster collected from the Southern Sea near Pusan", Kor. J. Parasitol., 30(4), pp.263~267 (1992)
- 서정수, "한국산 연어의 아니사키스형 유충의 감염현황", 한국어병학회지, 23(1), pp.123~129 (2010), 한국어병학회
- Suzuki, J., "Detection rate on the larvae of Anisakis simplex from salmon (Oncorhynchus spp.) retailed in Tokyo during 1996∼2001", Ann. Rep. Tokyo Metr. Res. Lab. P. H., 52, pp.26~30 (2001)
- Suzuki, J., "Risk factors for human Anisakis infection and association between the geographic origins of Scomber japonicus and anisakid nematodes", Int. J. Food. Microbiol., 137, pp.88~93 (2010)
- Umehara, A., "Molecular identification of Anisakis simplex sensu stricto and Anisakis pegreffii (Nematoda: Anisakidae) from fish and cetacean in Japanese waters", Parasitol. Int., 55, pp.267~271 (2006)
- Umehara, A., "Multiplex PCR for identification of Anisakis simplex sensu stricto, Anisakis pegreffii and the other anisakid nematodes", Prasitol. Int., 57, pp.49~53 (2008)
- Wasko, A.P., "Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales", Hereditas., 138, pp.161~165 (2003)
- Woo, H.C., "The infection status and identification of Anisakid larvae in marine fish caught from the sea near Cheju island", Kor. J. Vet. Publ. Hlth., 24(4), pp.307~317 (2000)
- Zhu, X.Q., "Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA", Parasitol. Res., 101, pp.1703~1707 (2007)
