A Sensitive and Specific Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Quantification of Salivary Melatonin and Cortisol: Development and Comparison With Immunoassays
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of salivary melatonin and cortisol concentrations according to the Clinical and Laboratory Standards Institute guidelines. Additionally, we compared the LC-MS/MS assay with immunoassays, ELISA (Direct Salivary Melatonin Elisa EK-DSM, Bühlmann Laboratories AG, Schönenbuch, Switzerland) and electrochemiluminescence immunoassay (Cortisol II, Roche, Mannheim, Germany), using 121 saliva samples. The LC-MS/MS assay exhibited good performance in terms of linearity, precision, accuracy, limit of detection, lower limit of quantification, extraction recovery, carry-over, and matrix effect. The LC-MS/MS assay and immunoassays showed strong correlation (Pearson’s r=0.910 for melatonin, r=0.955 for cortisol), but demonstrated a significant mean bias of 23.2% (range 54.0–143.7%) for melatonin and 48.9% (range 59.7–184.7%) for cortisol. Our LC-MS/MS assay provided more sensitive and reliable salivary melatonin and cortisol quantification results compared with immunoassays.
Abstract
Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of salivary melatonin and cortisol concentrations according to the Clinical and Laboratory Standards Institute guidelines. Additionally, we compared the LC-MS/MS assay with immunoassays, ELISA (Direct Salivary Melatonin Elisa EK-DSM, Bühlmann Laboratories AG, Schönenbuch, Switzerland) and electrochemiluminescence immunoassay (Cortisol II, Roche, Mannheim, Germany), using 121 saliva samples. The LC-MS/MS assay exhibited good performance in terms of linearity, precision, accuracy, limit of detection, lower limit of quantification, extraction recovery, carry-over, and matrix effect. The LC-MS/MS assay and immunoassays showed strong correlation (Pearson’s r=0.910 for melatonin, r=0.955 for cortisol), but demonstrated a significant mean bias of 23.2% (range 54.0–143.7%) for melatonin and 48.9% (range 59.7–184.7%) for cortisol. Our LC-MS/MS assay provided more sensitive and reliable salivary melatonin and cortisol quantification results compared with immunoassays.
참고문헌 (19)
- Claustrat B, "Melatonin: physiological effects in humans", Neurochirurgie, 61, pp.77~84 (2015)
- Debono M, "Modified-release hydrocortisone to provide circadian cortisol profiles", J Clin Endocrinol Metab, 94, pp.1548~1554 (2009)
- Blair J, "Salivary cortisol and cortisone in the clinical setting", Curr Opin Endocrinol Diabetes Obes, 24, pp.161~168 (2017)
- Voultsios A, "Salivary melatonin as a circadian phase marker: validation and comparison to plasma melatonin", J Biol Rhythms, 12, pp.457~466 (1997)
- Jung C, "Plasma, salivary and urinary cortisol levels following physiological and stress doses of hydrocortisone in normal volunteers", BMC Endocr Disord, 14, pp.91~ (2014)
- Taylor AE, "Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow", Eur J Endocrinol, 173, pp.D1~D12 (2015)
- Kennaway DJ, "A critical review of melatonin assays: past and present", J Pineal Res, 67, pp.e12572~ (2019)
- Doi M, "Latenight salivary cortisol as a screening test for the diagnosis of Cushing’s syndrome in Japan", Endocr J, 55, pp.121~126 (2008)
- Turpeinen U, "Determination of cortisol in serum, saliva and urine", Best Pract Res Clin Endocrinol Metab, 27, pp.795~801 (2013)
- van Faassen M, "Relationship between plasma and salivary melatonin and cortisol investigated by LC-MS", MS, 2017, pp.55, 1340~1348
- Fustinoni S, "High-throughput determination of cortisol, cortisone, and melatonin in oral fluid by on-line turbulent flow liquid chromatography interfaced with liquid chromatography", tandem mass spectrometry, 2013, pp.27, 1450~1460
- Jensen MA, "Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone", J Chromatogr B Analyt Technol Biomed Life Sci, 879, pp.2527~2532 (2011)
- Jensen MA, "An interlaboratory comparison between similar methods for determination of melatonin, cortisol and testosterone in saliva", Scand J Clin Lab Invest, 74, pp.454~461 (2014)
- CLSI, "Liquid chromatography-mass spectrometry methods" (2014), Clinical and Laboratory Standards Institute
- Vogeser M, "Multicenter performance evaluation of a second generation cortisol assay", Clin Chem Lab Med, 55, pp.826~835 (2017)
- Shirtcliff EA, "Use of salivary biomarkers in biobehavioral research: cotton-based sample collection methods can interfere with salivary immunoassay results", Psychoneuroendocrinology, 26, pp.165~173 (2001)
- Nölte I, "Pineal volume and circadian melatonin profile in healthy volunteers:an interdisciplinary approach", J Magn Reson Imaging, 30, pp.499~505 (2009)
- Riemann D, "Nocturnal cortisol and melatonin secretion in primary insomnia", Psychiatry Res, 113, pp.17~27 (2002)
- Corbalán-Tutau D, "Daily profile in two circadian markers “melatonin and cortisol” and associations with metabolic syndrome components", Physiol Behav, 123, pp.231~235 (2014)