Quantitative measurement of salivary testosterone in Korean adults by stable isotope-dilution liquid chromatographyelectrospray- tandem mass spectrometry
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초록
Salivary testosterone levels in Korean adults were quantitatively measured for the first time by liquid chromatography-electrospray-tandem mass spectrometry (LC ESI MS/MS). Salivary testosterone was separated on a multiple reaction monitoring (MRM) chromatogram within 7 min. The LC ESI MS/MS assay was validated over the linearity range of 0.01-2.00 ng/ml (r=0.99987) using testosterone-d3 as an internal standard. The lower limit of quantification (LOQ) was 0.01ng/ml. The intra- and inter-assay precisions were 1.54% to 4.09% and 0.96% to 4.29%, respectively. The mean recovery was 93.32% (range 88.43-98.05%). The validated assay was then applied to measure the salivary testosterone levels of Korean adults. In men, the salivary testosterone level collected between 9：00-11：00 am was approximately 2.8 times higher than that in women (P ＜ 0.0001). Salivary testosterone levels in both sexes negatively correlated with age. The present assay would also be useful in measuring salivary testosterone levels in clinical laboratories.
Abstract
Salivary testosterone levels in Korean adults were quantitatively measured for the first time by liquid chromatography-electrospray-tandem mass spectrometry (LC ESI MS/MS). Salivary testosterone was separated on a multiple reaction monitoring (MRM) chromatogram within 7 min. The LC ESI MS/MS assay was validated over the linearity range of 0.01-2.00 ng/ml (r=0.99987) using testosterone-d3 as an internal standard. The lower limit of quantification (LOQ) was 0.01ng/ml. The intra- and inter-assay precisions were 1.54% to 4.09% and 0.96% to 4.29%, respectively. The mean recovery was 93.32% (range 88.43-98.05%). The validated assay was then applied to measure the salivary testosterone levels of Korean adults. In men, the salivary testosterone level collected between 9：00-11：00 am was approximately 2.8 times higher than that in women (P ＜ 0.0001). Salivary testosterone levels in both sexes negatively correlated with age. The present assay would also be useful in measuring salivary testosterone levels in clinical laboratories.
참고문헌 (20)
- Burger, H., "Androgen production in women", Fertil. Steril., 77(4), pp.3~5 (2002)
- Griffin, J. E., "Disorders of the testes and the male reproductive tract; in Williams Textbook of Endocrinology, 9th ed", pp.819~875 (1998), Saunders Company
- Synder, P., "Editorial: The role of androgens in women", J. Clin. Endocrinol. Metab., 86, pp.1006~1007 (2001)
- Matsui, F., "Liquid chromatography-tandem mass spectrometry (LC-MS", MS) assay for simultaneous measurement of salivary testosterone and cortisol in healthy men for utilization in the diagnosis of late-onset hypogonadism in males, 2009, pp.56, 1083~1093
- Shibayamaa, Y., "Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS", MS: Method development and evaluation of applicability for diagnosis and medication for late-onset hypogonadism, 2009, pp.877, 2615~2623
- John, E., "Validation of salivary testosterone as a screening test for male hypogonadism", Aging Male, 9, pp.165~169 (2006)
- Goncharov, N., "Diagnostic significance of free salivary testosterone measurement using a direct luminescence immunoassay in healthy men and in patients with disorders of androgenic status", Aging Male, 9, pp.111~122 (2006)
- Karrer-Voegeli, S., "Androgen dependence of hirsutism, acne, and alopecia in women: retrospective analysis of 228 patients investigated for hyperandrogenism", Medicine (Baltimore), 88, pp.32~45 (2009)
- Swinkels, L. M., "Concentrations of salivary testosterone and plasma total, non-sex-hormone-binding globulin-bound, and free testosterone in normal and hirsute women during administration of dexamethasone", synthetic corticotropin, 1991, pp.37, 180~185
- Dunn, J. F, "Transport of steroid hormones: binding of 21 endogenous steroids to both eestosterone-binding globulin and corticosteroid-binding globulin in human plasma", J. Clin. Endocrinol. Metab., 53, pp.58~68 (1981)
- Morley, J. E., "The diagnosis of late life hypogonadism", Aging Male, 10, pp.217~220 (2007)
- Yasuda, M., "Diagnostic significance of salivary testosterone measurement revisited: using liquid chromatography", mass spectrometry and enzyme-linked immunosorbent assay, 2008, pp.5, 56~63
- Arregger, A. L., "Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism", Clin. Endocrinol., 67, pp.656~662 (2007)
- Shirtcliff, E. A., "Gender differences in the validity of testosterone measured in saliva by immunoassay", Horm. Behav., 42, pp.62~69 (2002)
- Taieb, J., "Testosterone measured by 10 immunoassays and by isotope-dilution gas chromatography-mass spectrometry in sera from 116 men, women, and children", Clin. Chem., 49, pp.1381~1395 (2003)
- Herold, D., "Immunoassays for testosterone in women: better than guessing?", Clin. Chem., 49, pp.1250~1251 (2003)
- Wang, C., "Measurement of total serum testosterone in adult men: comparison of current laboratory methods versus liquid chromatography-tandem mass spectrometry", J. Clin. Endocrinol. Metab., 89, pp.534~543 (2004)
- Kushnir, M. M, "Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals", Clin. Chem., 56, pp.1138~1147 (2010)
- Ellison, P. T., "Population variation in age-related decline in male salivary testoterone", Hum. Reprod., 17, pp.3251~3253 (2002)
- "Guidance for Industry: Bioanalytical Method Validation" (2001), Department of Health and Human Services. F.D.A