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Tissue Fluid Enzyme-Linked Immunosorbant assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets

윤희정|김동수|김선민|이지윤|김태임|한정희|조윤상|강승원|곽동미|우호춘|원은정|홍성종|남호우|Fuhong Dai|Xing-Quan Zhu|Won Gi Yoo|Chun-Ren Wang
대한기생충학ㆍ열대의학회 (The Korean Society For Parasitology and Tropical Medicine)|2018.10|Parasites, Hosts and Diseases|제56권 제5호|pp. 437-446

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참고문헌

키워드

KoreanELISAporkToxoplasma gondiitissue fluidimported
주제분류예방의학/직업환경의학

초록

To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzo- ites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066- 0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative pre- dictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a com- mercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to sup- ply safe pork on the tables.

Abstract

To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzo- ites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066- 0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative pre- dictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a com- mercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to sup- ply safe pork on the tables.

참고문헌 (44)

  1. Hill DE, "Biology and epidemiology of Toxoplasma gondii in man and animals", Anim Health Res Rev, 6, pp.41~61 (2005)
  2. Robert-Gangneux F, "Epidemiology of and diagnostic strategies for toxoplasmosis", Clin Microbiol Rev, 25, pp.264~296 (2012)
  3. Choi WY, "Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary's Hospital and Cheju Medical Center", Korean J Prasitol, 27, pp.171~175 (1989)
  4. Choi WY, "Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzymelinked immunosorbent assay", Korean J Prasitol, 30, pp.83~90 (1992)
  5. 송경주, "Seroprevalence of toxoplasmosis in Korean pregnant women", Parasites, Hosts and Diseases, 43(2), pp.1~10 (2005), 대한기생충학ㆍ열대의학회
  6. 홍성종, "Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea", Parasites, Hosts and Diseases, 49(3), pp.309~311 (2011), 대한기생충학ㆍ열대의학회
  7. 안혜진, "Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea", Parasites, Hosts and Diseases, 50(3), pp.225~227 (2012), 대한기생충학ㆍ열대의학회
  8. Zhaoshou Yang, "A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea", Parasites, Hosts and Diseases, 50(3), pp.191~197 (2012), 대한기생충학ㆍ열대의학회
  9. Djurković-Djaković O, "Pork as a source of human parasitic infection", Clin Microbiol Infect, 19, pp.586~594 (2013)
  10. Hill DE, "Toxoplasma gondii prevalence in farm animals in the United States", Int J Parasitol, 43, pp.107~113 (2013)
  11. Jones JL, "Foodborne toxoplasmosis", Clin Infect Dis, 55, pp.845~851 (2012)
  12. Wingstrand A, "Clinical observations, pathology, bioassay in mice and serological response at slaughter in pigs experimentally infected with Toxoplasma gondii", Vet Parasitol, 72, pp.129~140 (1997)
  13. 임혜미, "Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea", Parasites, Hosts and Diseases, 50(4), pp.287~293 (2012), 대한기생충학ㆍ열대의학회
  14. Fan CK, "Serological survey of Toxoplasma gondii infection among slaughtered pigs in northwestern Taiwan", J Parasitol, 90, pp.653~654 (2004)
  15. Han K, "Seroprevalence of Toxoplasma gondii infection and risk factors associated with seropositivity of pregnant women in Korea", J Parasitol, 94, pp.963~965 (2008)
  16. Forbes LB, "Performance of commercial ELISA and agglutination test kits for the detection of anti-Toxoplasma gondii antibodies in serum and muscle fluid of swine infected with 100, 300, 500 or 1000 oocysts", Vet Parasitol, 190, pp.362~367 (2012)
  17. Villena I, "New strategy for the survey of Toxoplasma gondii in meat for human consumption", Vet Parasitol, 183, pp.203~208 (2012)
  18. Sroka J, "Comparison of the efficiency of two commercial kits - ELFA and Western blot in estimating the phase of Toxoplasma gondii infection in pregnant women", Ann Agric Environ Med, 23, pp.570~575 (2016)
  19. Rostami A, "Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection", Infection, 25, pp.264~ (2018)
  20. Glor SB, "Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep", Parasit Vectors, 6, pp.85~ (2013)
  21. Meemken D, "Establishment of serological herd profiles for zoonoses and production diseases in pigs by “meat juice multi-serology”", Prev Vet Med, 113, pp.589~598 (2014)
  22. 남호우, "Pro-inflammatory Cytokine Expression of Spleen Dendritic Cells in Mouse Toxoplasmosis", Parasites, Hosts and Diseases, 49(2), pp.109~112 (2011), 대한기생충학ㆍ열대의학회
  23. Kim TY, "Recombinant Paragonimus westermani yolk ferritin is a useful serodiagnostic antigen", J Infect Dis, 185, pp.1373~1375 (2002)
  24. Gamble HR, "Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig", Vet Parasitol, 128, pp.177~181 (2005)
  25. Hill DE, "Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine", Vet Parasitol, 141, pp.9~17 (2006)
  26. Lind P, "The time course of the specific antibody response by various ELISAs in pigs experimentally infected with Toxoplasma gondii", Vet Parasitol, 71, pp.1~15 (1997)
  27. Basso W, "Assessment of diagnostic accuracy of a commercial ELISA for the detection of Toxoplasma gondii infection in pigs compared with IFAT, Tg-SAG1-ELISA and Western blot, using a Bayesian latent class approach", Int J Parasitol, 43, pp.565~570 (2013)
  28. Dubey JP, "Toxoplasma gondii infection in humans and animals in the United States", Int J Parasitol, 38, pp.1257~1278 (2008)
  29. Dzib-Paredes GF, "Seroprevalence and parasite load of Toxoplasma gondii in Mexican hairless pig(Sus scrofa)tissues from the Southeast of Mexico", Vet Parasitol, 229, pp.45~49 (2016)
  30. Jiang T, "Evaluation of a recombinant MIC3 based latex agglutination test for the rapid serodiagnosis of Toxoplasma gondii infection in swines", Vet Parasitol, 158, pp.51~56 (2008)
  31. Lundén A, "Serological survey of Toxoplasma gondii infection in pigs slaughtered in Sweden", Scand J Infect Dis, 34, pp.362~365 (2002)
  32. Dubey JP, "Toxoplasmosis of Animals and Humans" (2016), CRC Press
  33. Opsteegh M, "Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR", Int J Food Microbiol, 139, pp.193~201 (2010)
  34. Juránková J, "Brain is the predilection site of Toxoplasma gondii in experimentally inoculated pigs as revealed by magnetic capture and real-time PCR", Food Microbiol, 38, pp.167~170 (2014)
  35. Felin E, "Comparison of commercial ELISA tests for the detection of Toxoplasma antibodies in the meat juice of naturally infected pigs", Vet Parasitol, 238, pp.30~34 (2017)
  36. 서민구, "경북 동부지역 소와 돼지에서의 톡소포자충 항체 조사", 한국동물위생학회지, 32(2), pp.131~137 (2009), 한국동물위생학회
  37. 김영훈, "Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up", Parasites, Hosts and Diseases, 55(3), pp.247~254 (2017), 대한기생충학ㆍ열대의학회
  38. Ortega-Pacheco A, "Infection dynamic of Toxoplasma gondii in two fattening pig farms exposed to high and low cat density in an endemic region", Vet Parasitol, 175, pp.367~371 (2011)
  39. 황주선, "Infections by pathogens with different transmission modes in feral cats from urban and rural areas of Korea", Journal of Veterinary Science, 18(4), pp.541~545 (2017), 대한수의학회[DOI]
  40. Alvarado-Esquivel C, "High prevalence of Toxoplasma gondii antibodies in domestic pigs in Oaxaca State, Mexico", J Parasitol, 98, pp.1248~1250 (2012)
  41. Herrero L, "Toxoplasma gondii in raw and dry-cured ham: the influence of the curing process", Food Microbiol, 65, pp.213~220 (2017)
  42. Hill DE, "Seroprevalence of and risk factors for Toxoplasma gondii in the US swine herd using sera collected during the National Animal Health Monitoring Survey (Swine 2006)", Zoonoses Public Health, 57, pp.53~59 (2010)
  43. Djokic V, "Toxoplasma gondii infection in pork produced in France", Parasitology, 143, pp.557~567 (2016)
  44. Muñoz-Zanzi C, "Detection of oocyst-associated toxoplasmosis in swine from southern Chile", Zoonoses Public Health, 59, pp.389~392 (2012)

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