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헌혈혈액에서 Transfusion-transmitted Virus 유전자그룹의 분포

Distribution of Transfusion-transmitted Virus Genomic Groups in Blood Products

한태희|성희경|정주영
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2005.04|Annals of Laboratory Medicine|제25권 제2호|pp. 116-122

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초록

배경 : Transfusion-transmitted virus (TTV)는 무외피 단일가닥 DNA 바이러스로 수혈을 통해 전파될 수 있다. 국내에서 헌혈자와 혈액제제에서 TTV 감염률이 조사된 적이 있으나 혈액제제에 감염된 TTV의 유전자 그룹(1-5)에 대한 연구가 이루어지지 않아 저자들은 그 분포를 조사하고자 하였다.방법 : 전혈, 농축적혈구 각 25단위의 혈액백 분절에서 혈장을 채취하여 DNA를 추출한 후 TV에 대한 두 종류의 시발체(A, B)와 유전자그룹 1-5 특이 시발체를 이용하여 중합효소연쇄반응을 시행하여 유전자그룹을 분석하였다.결과 : 모든 시발체를 이용하여 혈액제제의 96% (48/50)에서TV가 검출되었다. 혈액제제에 감염된 TV의 유전자그룹은그룹 3 (52%), 그룹 4 (46%), 그룹 1 (20%), 그룹 5 (10%),그룹 2 (2%)였다. 혈액제의 14% (7/50)에서 TTV가 시발체A 혹은 B 검출되었으나 유전자그룹 특이 시발체로는 증폭되지헌혈혈액에서 Transfusion-transmitted virus유전자그룹의 분포 121않았다. 한 개의 유전자그룹에 감염된 경우는 44% (22/50), 두개의 유전자그룹에 감염된 경우는 28% (14/50), 세 개의 유전자그룹에 감염된 경우는 10% (5/50)이고 네 개 이상의 유전자그룹에 감염된 경우는 없었다. 결론 : 국내에서는 대부분의 혈액제제에서 TTV가 검출되었으며 그룹 1에서 그룹 5까지의 모든 유전자그룹이 검출되었다.

Abstract

Background : Transfusion-transmitted virus (TTV) is a small DNA virus with single-stranded, closed circular, antisense genome infecting humans. The TTV has been classified into five major genomic groups 1-5. There have been a few studies on TTV prevalence in blood donors and blood products in Korea. However there have been no reports on the TTV genomic groups in Korea. The aim of this study was to gain information on TTV genomic groups in blood products in Korea. Methods : A total of 50 plasma samples from blood products (25 units each of red blood cell and whole blood) were tested. The samples are obtained from the segments of the blood products. TTV DNA was detected using polymerase chain reaction (PCR) with two sets of universal primers (A set and B set), and TTV genomic groups were determined using PCR with group specific primer sets. Results : TTV DNA was detected in 96% (48/50) of the blood products: the TTV genomic group 3 was found the most frequently (52%, 26/50), followed by group 4 (46%, 23/50), group 1 (20%, 10/50), group 5 (10%, 5/20), and group 2 (2%, 1/50). There were seven blood products (14%) infected with TTVs but their genomic groups were not identified with group specific primer sets. Among the blood products, 44% (22/50) were infected with a unique TTV genomic group; 38% (19/50) were coinfected with TTV from 2 (28%, 14/50) or 3 (10%, 5/50) genomic groups. Conclusions : Blood products are frequently infected with TTV and all five known genomic groups are detected in Korea.

참고문헌 (26)

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관련 논문AI추천

헌혈혈액에서 Transfusion-transmitted virus (TTV)와TTV-like mini virus (TLMV)의 감염김문정, 백인기, 상희경, 정주영, 한태희대한진단검사의학회 · Annals of Laboratory Medicine2004 · 제24권 제4호한국인 건강 성인과 B형 및 C형 간염 바이러스 감염자에서의 시발체에 따른 TT Virus 양성률강희정, 김재석, 김한성, 송원근, 이규만대한임상미생물학회 · Annals of Clinical Microbiology2007 · 제10권 제1호일반인, 만성 간질환, 혈액투석 및 수혈 받은 환자에서의 TT virus(TTV) 감염이난영, 이원길, 정규영, 허운보대한임상미생물학회 · Annals of Clinical Microbiology2006 · 제9권 제1호Investigation of the Prevalence of Human Parvovirus B19 DNA in Korean Plasmapheresis Donors강재원, 권소영, 오덕자, 이유라, 조남선대한진단검사의학회 · Annals of Laboratory Medicine2010 · 제30권 제1호적혈구 농축제재에서 HBV DNA의 노출정도김정숙, 송달효, 이채훈영남대학교 의과대학 · Journal of Yeungnam Medical Science1995 · 제12권 제2호소아의 Transfusion Transmitted Virus-Like Minivirus 유병률정주영, 한태희대한소아감염학회 · Pediatric Infection and Vaccine2004 · 제11권 제2호

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