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유세포분석기를 이용한 혈소판 활성화 표지자 참고치 산정

Establishment of Reference Values for Platelet Activation Markers by Flow Cytometry

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참고문헌

주제분류병리학

초록

배경 : 유세포분석기를 이용한 혈소판기능 검사시 도움을 주고자 혈소판 활성화 표지자와 백혈구-혈소판 응집의 참고치를 산정하였다. 방법 : 헌혈 지원자 30명을 대상으로 희석된 전혈에 20 MADP 혹은 100 M TRAP를 첨가하지 않은 기저상태 혹은 첨가한 활성화상태 모두 PAC1 혹은 CD62P 항체로 염색하였고,백혈구-혈소판 응집은 전혈에 CD41a, CD14, CD45 항체를 동시에 반응시킨 후 유세포분석기를 이용하여 각 표지자의 양성률(%)을 구하였다. 결과 : PAC1및 CD62P의 참고치는 기저상태에서 0.1-12.5%,0.0-4.7%, 20 M ADP 투여 후 65.3-92.4%와 39.0-75.7%, 100M TRAP 투여 후 68.1-93.1%와 60.5-91.2%였으며, 백혈구-혈소판 응집 2.8-23.6%, 과립구-혈소판 5.3-34.2%, 림프구-혈소판 응집 4.9-21.6%였다.

참고문헌 (16)

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