유세포분석법을 이용한 말초혈액 림프구아형 검사의 정상 참고치

Abstract

To estimate the reference values of peripheral blood lymphocyte subsets, authors meas-ured CD3+, CD4+/CD3+, CD8+/CD3+, CD+-/CDI6+ or CD56+ and CDI9+ cells in 37 healthy adults (29 males, 8 females, aged from 20 to 49 years) and 12 children (3 males, 9 females aged from 3 months to 7 years). EDTA blood samples were obtained from healthy subjects who had no history of viral or other illnesses or medication in Asan Medical Center during the period of January to November, 2001. Measurement of peripheral blood lymphocytes subsets was performed by flow cytometry (FACScan, Becton-Dickinson Co., USA). The reference ranges follow; for healthy adults T cell (CD3+), 54-82%; T helperjinducer (Th/i cell) (CD4+/CD3+), 24-55%; T suppressor (Ts/c)cell (CD8+/ CD3+), 11-38%; B cell (CD19+), 5-23%; NK cell (CD3-/16+ or 56+), 5-35%; and for children T cell (CD3+),60-82%; Th/i cell (CD4+/CD3+), 24-49%; Ts/c cell (CD8+/CD3+), 15-35%; B cell (CD19+), 11-27%; and NK cell(CD3-/16+ or 56+), 4-20%. In healthy adults the mean percentages of T helper/inducer cells and natural killer cells showed an increasing tendency related to increasing ages, and those of T suppressor/cytotoxic cells showed the reverse results. In children the percentages of natural killer cells showed lower results and those of B cells showed higher results than in adults. Our data obtained by the measurement df peripheral blood lymphocyte subsets will serve as the reference values in adults and children.