Clinical Usefulness of LabChip Real-Time PCR Using Lab-On-a-Chip Technology for Ddiagnosing Malaria
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
As malaria remains a major health problem worldwide, various diagnostic tests have been developed, including microscopy-based and rapid diagnostic tests. LabChip real-time PCR (LRP) is a small and portable device used to diagnose malaria using lab-on-a-chip technology. This study aimed to evaluate the diagnostic performance of LRP for detecting malaria parasites. Two hundred thirteen patients and 150 healthy individuals were enrolled from May 2009 to October 2015. A diagnostic detectability of LRP for malaria parasites was compared to that of conventional RT-PCR. Sensitivity of LRP for Plasmodium vivax, P. falciparum, P. malariae, and P. ovale was 95.5%, 96.0%, 100%, and 100%, respectively. Specificity of LRP for P. vivax, P. falciparum, P. malariae, and P. ovale was 100%, 99.3%, 100%, and 100%, respectively. Cohen’s Kappa coefficients between LRP and CFX96 for detecting P. vivax, P. falciparum, P. malariae, and P. ovale were 0.96, 0.98, 1.00, and 1.00, respectively. Significant difference was not observed between the results of LRP and conventional RT-PCR and microscopic examination. A time required to amplify DNAs using LRP and conventional RT-PCR was 27 min and 86 min, respectively. LRP amplified DNAs 2 times more fast than conventional RT-PCR due to the faster heat transfer. Therefore, LRP could be employed as a useful tool for detecting malaria parasites in clinical laboratories.
Abstract
As malaria remains a major health problem worldwide, various diagnostic tests have been developed, including microscopy-based and rapid diagnostic tests. LabChip real-time PCR (LRP) is a small and portable device used to diagnose malaria using lab-on-a-chip technology. This study aimed to evaluate the diagnostic performance of LRP for detecting malaria parasites. Two hundred thirteen patients and 150 healthy individuals were enrolled from May 2009 to October 2015. A diagnostic detectability of LRP for malaria parasites was compared to that of conventional RT-PCR. Sensitivity of LRP for Plasmodium vivax, P. falciparum, P. malariae, and P. ovale was 95.5%, 96.0%, 100%, and 100%, respectively. Specificity of LRP for P. vivax, P. falciparum, P. malariae, and P. ovale was 100%, 99.3%, 100%, and 100%, respectively. Cohen’s Kappa coefficients between LRP and CFX96 for detecting P. vivax, P. falciparum, P. malariae, and P. ovale were 0.96, 0.98, 1.00, and 1.00, respectively. Significant difference was not observed between the results of LRP and conventional RT-PCR and microscopic examination. A time required to amplify DNAs using LRP and conventional RT-PCR was 27 min and 86 min, respectively. LRP amplified DNAs 2 times more fast than conventional RT-PCR due to the faster heat transfer. Therefore, LRP could be employed as a useful tool for detecting malaria parasites in clinical laboratories.
참고문헌 (25)
- Nafo Traoré F, "Rolling back malaria: opportunities and challenges", Trans R Soc Trop Med Hyg, 99, pp.403~406 (2005)[DOI]
- Kim TS, "Decreasing incidence of Plasmodium vivax in the Republic of Korea during 2010-2012", Malar J, 12, pp.309~ (2013)[DOI]
- "Infectious Diseases Portal"[URL]
- Moody A, "Rapid diagnostic tests for malaria parasites", Clin Microbiol Rev, 15, pp.66~78 (2002)[DOI]
- Amexo M, "Malaria misdiagnosis:effects on the poor and vulnerable", Lancet, 364, pp.1896~1898 (2004)[DOI]
- Kim KH, "Evaluation of four rapid diagnostic tests for the diagnosis of Plasmodium vivax in Korea", Trop Med Int Health, 16, pp.1427~1431 (2011)[DOI]
- Gillet P, "Assessment of the prozone effect in malaria rapid diagnostic tests", Malar J, 8, pp.271~ (2009)[DOI]
- Pati P, "High proportions of pfhrp2 gene deletion and performance of HRP2-based rapid diagnostic test in plasmodium falciparum field isolates of Odisha", Malar J, 17, pp.394~ (2018)[DOI]
- Kim JY, "Development and efficacy of real-time PCR in the diagnosis of vivax malaria using field samples in the Republic of Korea", PLoS One, 9, pp.e105871~ (2014)[DOI]
- Haeberle S, "Microfluidic platforms for lab-on-a-chip applications", Lab Chip, 7, pp.1094~1110 (2007)[DOI]
- Niemz A, "Point-of-care nucleic acid testing for infectious diseases", Trends Biotechnol, 29, pp.240~250 (2011)[DOI]
- Coarsey CT, "Strategies in ebola virus disease (EVD) diagnostics at the point of care", Crit Rev Microbiol, 43, pp.779~798 (2017)[DOI]
- Darwish NT, "Point-of-care tests: a review of advances in the emerging diagnostic tools for dengue virus infection", Sens Actuators B Chem, 255, pp.3316~3331 (2018)[DOI]
- Duchesne L, "Innovative technologies for point-ofcare testing of viral hepatitis in low-resource and decentralized settings", J Viral Hepat, 25, pp.108~117 (2018)[DOI]
- Mauk M, "Miniaturized devices for point of care molecular detection of HIV", Lab Chip, 17, pp.382~394 (2017)[DOI]
- Lee SH, "Rapid detection of Mycobacterium tuberculosis using a novel ultrafast chip-type real-time polymerase chain reaction system", Chest, 146, pp.1319~1326 (2014)[DOI]
- Rougemont M, "Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific realtime PCR assays", J Clin Microbiol, 42, pp.5636~5643 (2004)[DOI]
- Lu F, "Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China", Parasitol Res, 110, pp.2439~2444 (2012)[DOI]
- Chen JH, "Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)", Acta Trop, 113, pp.61~65 (2010)[DOI]
- Hwang SY, "A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals", Acta Trop, 120, pp.40~45 (2011)[DOI]
- Alam MS, "Real-time PCR assay and rapid diagnostic tests for the diagnosis of clinically suspected malaria patients in Bangladesh", Malar J, 10, pp.175~ (2011)[DOI]
- Wang B, "Comparison of microscopy, nested-PCR, and real-time-PCR assays using high-throughput screening of pooled samples for diagnosis of malaria in asymptomatic carriers from areas of endemicity in Myanmar", J Clin Microbiol, 52, pp.1838~1845 (2014)[DOI]
- Taylor BJ, "A lab-on-chip for malaria diagnosis and surveillance", Malar J, 13, pp.179~ (2014)[DOI]
- Vo TK, "Evaluation of a real-time PCR assay for malaria diagnosis in patients from Vietnam and in returned travellers", Trans R Soc Trop Med Hyg, 101, pp.422~428 (2007)
- Song HO, "Polymeric LabChip real-time PCR as a point-of-care-potential diagnostic tool for rapid detection of influenza A", H1N1 virus in human clinical specimens, 2012, pp.7, e53325~
