본문 바로가기
KERD
논문KCI 등재

혈소판의 냉동보존에 미치는 일산화질소의 효과

Effect of Nitric Oxide on the Cryopreservation of Platelets

김정태|이재현|조용곤
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2008.04|Annals of Laboratory Medicine|제28권 제2호|pp. 136-143

2

조회

0

다운로드

1

인용

0

참고문헌

주제분류병리학

초록

Abstract

Background : To determine whether nitric oxide (NO) could inhibit activation of platelets stored in a cold or frozen state, we measured platelet P-selectin expression and platelet-bound fibrinogen in platelet-rich plasma (PRP) with S-nitrosoglutathione (GSNO) (Sigma, USA) by flow cytometry. Methods : PRP was prepared by centrifuging venous blood collected in a 3.2% sodium citrate tube from 10 healthy donors. It was aliquotted into 4 groups (no cryoprotectant, GSNO, GSNO/ dimethyl sulfoxide [DMSO] [Sigma], and DMSO), and stored at room, cold and freezing temperatures for 24 hrs. We performed a flow cytometric analysis of all specimens stained with FITC-fibrinogen and PE-CD62P monoclonal antibodies (Becton Dickinson, USA). The results were compared according to the storage temperature and agonist among 4 groups. Results : GSNO inhibited significantly the activation of frozen platelets, but not in the presence of DMSO. GSNO was also shown to preserve the aggregability of frozen platelets because in the presence of GSNO the delta percent change of P-selectin expression and fibrinogen binding of frozen platelets increased significantly irrelevant to DMSO. Conclusions : GSNO inhibited the activation of frozen platelets and preserved the platelet aggregability; therefore, it may be used as a protectant for platelet cryopreservation. (Korean J Lab Med 2008;28:136-43)

참고문헌 (34)

  1. Liu JH, "CD62p expression in platelet during the preparation course of Cryopreservated platelet-rich plasma", Zhongguo Shi Yan Xue Ye Xue Za Zhi, 10, pp.253~256 (2002)
  2. Armitage WJ, "Osmotic stress as a factor in the detrimental effect of glycerol on human platelets", Cryobiology, 23, pp.116~125 (1986)
  3. Arnaud FG, "Some effects of propane-1,2-diol on human platelets", Cryobiology, 27, pp.119~129 (1990)
  4. Dayian G, "A simplified method for freezing human blood platelets in glycerol-glucose using a statically controlled cooling rate device", Transfusion, 19, pp.255~260 (1979)
  5. Smillie JA, "Cryopreservation of human platelets with polyvinylpyrrolidone", Transfusion, 21, pp.552~556 (1981)
  6. Angelini A, "Torlontano G. Evaluation of four different methods for platelet freezing. In vitro and in vivo studies", Vox Sang, 62, pp.146~151 (1992)
  7. Gao DY, "Development of optimal techniques for cryopreservation of human platelets. I. Platelet activation during cold storage (at 22 and 8 degrees C) and cryopreservation", Cryobiology, 38, pp.225~235 (1999)
  8. Lozano ML, "Gonzalez-Conejero R, Vicente V. Platelet cryopreservation using a reduced dimethyl sulfoxide concentration and second-messenger effectors as cryopreserving solution", Cryobiology, 39, pp.1~12 (1999)
  9. Borzini P, "Platelet cryopreservation using second-messenger effector and lowdose (2%) dimethyl sulfoxide. In vitro evaluation of post-thawing platelet activity with the platelet function analyzer", Haematologica, 85, pp.885~887 (2000)
  10. Xiao H, "Platelet cryopreservation using a combination of epinephrine and dimethyl sulfoxide as cryoprotectants", Cryobiology, 41, pp.97~105 (2000)
  11. Ekmekci H, "Comparison of platelet fibronectin, ADP-induced platelet aggregation and serum total nitric oxide (NOx) levels in angiographically determined coronary artery disease", Thromb Res, 117, pp.249~254 (2006)
  12. Marcondes S, "Cyclic GMP-independent mechanisms contribute to the inhibition of platelet adhesion by nitric oxide donor: a role for alpha-actinin nitration", Proc Natl Acad Sci USA, 103, pp.3434~3439 (2006)
  13. Stojanovic A, "A phosphoinositide 3-kinase-AKT-nitric oxidecGMP signaling pathway in stimulating platelet secretion and aggregation", J Biol Chem, 281, pp.16333~16339 (2006)
  14. Hagberg IA, "Inhalation of nitric oxide inhibits ADP-induced platelet aggregation and alphagranule release", Platelets, 10, pp.382~390 (1999)
  15. Buyukkagnici DI, "Effects of plasma nitric oxide levels on platelet activation in single donor apheresis and random donor concentrates", Transfus Apher Sci, 36, pp.73~78 (2007)
  16. Albert J, "A comparison of the antiplatelet effect of S-nitrosoglutathione in whole blood and platelet-rich plasma", Thromb Res, 102, pp.161~165 (2001)
  17. Xiao HY, "Generation of annexin V-positive platelets and shedding of microparticles with stimulus-dependent procoagulant activity during storage of platelets at 4 degrees C", Transfusion, 40, pp.420~427 (2000)
  18. 김신원, "유세포분석기를 이용한 혈소판 활성화 표지자 참고치 산정", 대한진단검사의학회지, 26(5), pp.323~328 (2006), 대한진단검사의학회
  19. Phillips DR, "The platelet membrane glycoprotein IIb-IIIa complex", Blood, 71, pp.831~843 (1988)
  20. Brecher ME, "Technical manual" (2005), AABB
  21. Owens M, "Cryopreserved platelets have decreased adhesive capacity", Transfusion, 31, pp.160~163 (1991)
  22. Fratantoni JC, "Dimethyl sulfoxide: effects on function of fresh platelets and on the viability of platelets in storage", Transfusion, 23, pp.109~113 (1983)
  23. Cetin M., "Effects of DMSO on platelet functions and P-selectin expression during storage", Transfus Apher Sci, 24, pp.261~267 (2001)
  24. Borzini P, "Platelet cryopreservation using dimethylsulfoxide", polyethylene glycol, Vox Sang, pp.1993, 64[DOI]
  25. Pedrazzoli P, "Transfusion of platelet concentrates cryopreserved with ThromboSol plus low-dose dimethylsulphoxide in patients with severe thrombocytopenia: a pilot study", Br J Haematol, 108통권 : 6, pp.653~659 (2000)
  26. Wong K, "Nitric oxide infusion alleviates cellular activation during preparation, leukofiltration and storage of platelets", Transfus Apher Sci, 30, pp.29~39 (2004)
  27. Langford EJ, "A study of platelet activation during human cardiopulmonary bypass and the effect of S-nitrosoglutathione", Thromb Haemost, 78, pp.1516~1519 (1997)
  28. Wallen NH, "Effects of treatment with oral isosorbide dinitrate on platelet function in vivo; a double-blind placebo-controlled study in patients with stable angina pectoris", Br J C Pharmacol, 38, pp.63~70 (1994)
  29. Karlberg KE, "Dose-dependent effect of intravenous nitroglycerin on platelet aggregation, and correlation with plasma glyceryl dinitrate concentration in healthy men", Am J Cardiol, 69, pp.802~805 (1992)
  30. Karlberg KE, "Effects of nitroglycerin on platelet aggregation beyond the effects of acetylsalicylic acid in healthy subjects", Am J Cardiol, 71, pp.361~364 (1993)
  31. Albert J, "Effects of inhaled nitric oxide compared with aspirin on platelet function in vivo in healthy subjects", Clin Sci, 91, pp.225~231 (1996)
  32. Albert J, "Inhaled nitric oxide does not influence bleeding time or platelet function in healthy volunteers", Eur J Clin Invest, 29, pp.953~959 (1999)
  33. Radomski MW, "S-nitroso-glutathione inhibits platelet activation in vitro and in vivo", Br J Pharmacol, 107, pp.745~749 (1992)
  34. Kelm M, "Control of coronary vascular tone by nitric oxide", Circ Res, 66, pp.1561~1575 (1990)

관련 논문AI추천

혈소판농축액 보관 시 교반 중단 및 백혈구의 제거가 혈소판의 품질에 미치는 영향노상근, 위재호, 정의석, 최원철대한진단검사의학회 · Annals of Laboratory Medicine2008 · 제28권 제3호5일 보존용 채혈백에 따른 보존일자별 농축혈소판의 생화학 및 성상 분석고광임, 김승곤, 김태전, 박성훈, 신종관, 양대형, 유태종, 이동범대한임상검사과학회 · 대한임상검사과학회지2002 · 제34권 제2호프로테오믹스 기법을 이용한 혈소판농축액의 보관 및 백혈구제거에 따른단백프로파일 변화 분석서인범, 유숙원, 조윤정대한진단검사의학회 · Annals of Laboratory Medicine2008 · 제28권 제1호Haemonetics MCS 3p에 의한 혈소판 성분채혈 후 혈소판 감소율, 회수량 및 효율에 대한 연구강명서, 김종화, 남정모, 이마화대한임상검사과학회 · 대한임상검사과학회지2006 · 제38권 제1호순환계에서 Nitric Oxide의 생리-병리학적 역할과 그 임상적 의의이광윤영남대학교 의과대학 · Journal of Yeungnam Medical Science1996 · 제13권 제2호혈소판농축혈장 내의 성장요소가 조골세포주의 활성도에 미치는 영향김영수, 김창회, 임영준, 장경수, 정태욱대한치과보철학회 · 대한치과보철학회지2004 · 제42권 제2호

이 간행물의 다른 논문 (11건)

광범위한 골수 침범을 동반한 ALK 양성 광범위 B형 대세포림프종 2예의세포형태학적 특징과 CLTC-ALK 재배열의 분자유전학적 분석정희숙, 김원석, 김희진, 김선희, 김기현pp. 89-94Denaturing HPLC를 이용한 리팜핀 내성 결핵균의 검출조민호, 이상현, 남윤형, 안영창, 권필승, 김종완, 박수민, 장원철pp. 95-102실시간-중합효소연쇄반응을 이용한 결핵균의 검출박경운, 이재호, 김성한, 송상훈, 김홍빈, 박성섭, 김의종, 송정한, 유광철, 허세란, 장호은pp. 103-108Rhinovirus, Human Metapneumovirus, Coronavirus 검출을 위한SeeplexTM RV Detection 키트의 평가김미나, 우영대, 성흥섭, 최병후, 박숙자pp. 109-117한국인에서 중중 자궁내막증 감수성과 HLA-A, B항원의 연관성 연구김성훈, 박명희, 황동희, 최영민pp. 118-123
원문 보기