혈소판농축액 보관 시 교반 중단 및 백혈구의 제거가 혈소판의 품질에 미치는 영향
Effect of Interrupted Agitation and Removal of Leukocyte on Platelet Quality during theStorage of Platelet Concentrates
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Abstract
Background : This study aimed to analyze the influence of the interruption of agitation and removal of leukocytes on platelet concentrates (PCs), and determine the maximum amount of time the agitation could be interrupted without impairing PCs’ effectiveness during the storage period. Methods : Four ABO-identical random donor platelets agitated for 24 hr were pooled, and divided into 4 units, and 2 units of them were leukoreduced. Then 52 pooled units were categorized into 4 groups, non-leukoreduced continuous agitation (Non-LRCA), non-leukoreduced interrupted agitation (Non-LRIA), leukoreduced continuous agitation (LRCA), and leukoreduced interrupted agitation (LRIA), and preserved for 6 days (total 7 days). Mean platelet volume (MPV), pH, HCO3 -, pO2, pCO2, CD62P, CD61, glucose, lactate, ammonia and free fatty acid were measured during the period. Results : Starting from the Day 4, the pH and HCO3 - of Non-LRIA group begun to decrease while the amount of lactate production, glucose consumption, and MPV increased compared to the Non- LRCA group (P<0.01). An increase in pO2 level was observed in the interrupted agitation groups as the storage period prolonged (P<0.01). The pH levels of all the units in the agitation groups remained higher than 6.4 up to Day 7, while those of the non-leukoreduction group did so only up to Day 2, but those of leukoreduction in the interrupted agitation groups did so up to Day 4. Conclusions : The interruption of agitation reduced the platelet’s capacity to utilize oxygen, increasing lactate amount and reducing pH level. However, the in vitro parameters of the Non-LRIA and Non-LRCA groups on Day 2 were similar to each other and the pH level remained at 6.4 or higher, making one day of agitation interruption possible after 24 hr of agitation. With leukocytes removed, the effective agitation interruption period may become longer. (Korean J Lab Med 2008;28:221-9)
참고문헌 (24)
- Simon TL, "Extension of platelet concentrate storage", Transfusion, 23, pp.207~212 (1983)
- Murphy S, "Platelet storage at 22 degrees C: role of gas transport across plastic containers in maintenance of viability", Blood, 46, pp.209~218 (1975)
- Murphy S, "Storage of platelet concentrates at 22 degrees C", Blood, 35, pp.549~557 (1970)
- Hunter S, "The effect of interruption of agitation on platelet quality during storage for transfusion", Transfusion, 41, pp.809~814 (2001)
- van der Meer PF, "Interruption of agitation of platelet concentrates:effects on in vitro parameters", Vox Sang, pp.227~234 (2005)
- Kilkson H, "Platelet metabolism during storage of platelet concentrates at 22 degrees C", Blood, pp.406~414 (1984)
- Hagberg IA, "Apheresisinduced platelet activation: comparison of three types of cell separators", Transfusion, 40, pp.182~192 (2000)
- Brecher ME, "Technical manual", pp.189~199 (2005), American Association of Blood Banks
- "Guide to the preparation use and quality assurance of blood components. Recommendation No. R(95)15. 12th ed. Strasbourg: Council of Europe, 2006:121-31", ~ (2006)
- de Wildt-Eggen J, "Improvement of platelet storage conditions by using new polyolefin containers", Transfusion, 37, pp.476~481 (1997)
- Moroff G, "The maintenance of platelet properties upon limited discontinuation of agitation during storage", Transfusion, 30, pp.427~430 (1990)
- Wallvik J, "The effect of different agitation modes on platelet metabolism, thromboxane formation, and alphagranular release during platelet storage", Transfusion, pp.639~643 (1990)
- Rinder HM, "Progressive platelet activation with storage: evidence for shortened survival of activated platelets after transfusion", Transfusion, 31, pp.409~414 (1991)
- Fijhneer R, "Detection of platelet activation with monoclonal antibodies and flow cytometry. Changes during platelet storage", Transfusion, 30, pp.20~25 (1990)
- Nieuwenhuis HK, "Studies with a monoclonal antibody against activated platelets: evidence that a secreted 53,000-molecular weight lysosome-like granule protein is exposed on the surface of activated platelets in the circulation", Blood, 70, pp.838~845 (1987)
- Kutlay S, "Influence of storage time on activation of platelets collected with CS 3000 Plus and Cobe Spec tra using platelet storage containers", Ther Apher, 6, pp.82~85 (2002)
- Cesar J, "Plasma free fatty acid metabolism during storage of platelet concentrates for transfusion", Transfusion, 27, pp.434~437 (1987)
- Holme S, "Evaluation of a new, more oxygen- permeable, polyvinylchloride container", Transfusion, 29, pp.159~164
- Snyder EL, "Extended storage of platelets in a new plastic container. II. In vivo response to infusion of platelets stored for 5 day", Transfusion, 25, pp.209~214 (1985)
- Kakaiya RM, "Platelet preservation in large containers", Vox Sang, 46, pp.111~118 (1984)
- Holme S, "Platelet storage at 22 degrees C for transfusion: interrelationship of platelet density and size, medium pH, and viability after in vivo infusion", J Lab Clin Med, 101, pp.161~174
- Fijnheer R, "Monitoring of platelet morphology during storage of platelet concentrates", Transfusion, 29, pp.36~40 (1989)
- Slichter SJ, "Preparation and storage of platelet concentrates. II. Storage variables influencing platelet viability and function", Br J Haematol, 34, pp.403~419 (1976)
- Kunicki TJ, "A study of variables affecting the quality of platelets stored at“ room temperature”", Transfusion, 15, pp.414~421 (1975)