Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA
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Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis byELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis stillsuffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. Thepresent study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen(ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA usingexcretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensisantigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that ofcrude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnosticantigen than CA for ELISA.
Abstract
Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis byELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis stillsuffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. Thepresent study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen(ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA usingexcretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensisantigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that ofcrude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnosticantigen than CA for ELISA.