Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)
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The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis andOpisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchiscontained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis34-37, 43, 70, and 100 kDa.Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchiswere major componentsof each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa ofOpisthorchiscross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted withsera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated withthe intensity of the liver fluke infection.
Abstract
The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis andOpisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchiscontained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis34-37, 43, 70, and 100 kDa.Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchiswere major componentsof each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa ofOpisthorchiscross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted withsera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated withthe intensity of the liver fluke infection.