Virulence Characteristics of Sucrose-fermenting Vibrio vulnificus Strains
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We identified 6 sucrose-fermenting Vibrio vulnificus strains and examined their virulence characteristics. They were all encapsulated, motile, capable of producing toxins and utilizing transferrin-bound iron, cytotoxic to cultured cells, and virulent enough to kill mice. They could be definitely identified only by genetic identification methods such as PCR, and not by conventional culture-based identification methods such as API 20E (bioMérieux, France). These results indicate that it is essential to adopt genetic approaches as early as possible in order to avoid misdiagnosis of such strains, especially in clinical situations. (Korean J Lab Med 2010;30:507-10)
Abstract
We identified 6 sucrose-fermenting Vibrio vulnificus strains and examined their virulence characteristics. They were all encapsulated, motile, capable of producing toxins and utilizing transferrin-bound iron, cytotoxic to cultured cells, and virulent enough to kill mice. They could be definitely identified only by genetic identification methods such as PCR, and not by conventional culture-based identification methods such as API 20E (bioMérieux, France). These results indicate that it is essential to adopt genetic approaches as early as possible in order to avoid misdiagnosis of such strains, especially in clinical situations. (Korean J Lab Med 2010;30:507-10)
참고문헌 (12)
- Jones MK, "Vibrio vulnificus: disease and pathogenesis", Infect Immun, 77, pp.1723~1733 (2009)
- Harwood VJ, "Methods for isolation and confirmation of Vibrio vulnificus from oysters and environmental sources:a review", J Microbiol Methods, 59, pp.301~316 (2004)
- Abbott SL, "Vibrio and related organisms. In: Murray PR, Baron EJ, et al. eds. Manual of clinical microbiology. 9th ed", American Society of Microbiology, 723~733 (2007)
- Nagao M, "Two cases of sucrose-fermenting Vibrio vulnificus infection in which 16S rDNA sequencing was useful for diagnosis", Jpn J Infect Dis, 59, pp.108~110 (2006)
- Reddy GP, "Purification and determination of the structure of capsular polysaccharide of Vibrio vulnificus M06-24", J Bacteriol, 174, pp.2620~2630 (1992)
- Dalsgaard A, "Comparison of a commercial biochemical kit and an oligonucleotide probe for identification of environmental isolates of Vibrio vulnificus", Lett Appl Microbiol, 22, pp.184~188 (1996)
- Hill WE, "Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters", Appl Environ Microbiol, 57, pp.707~711 (1991)
- Shao CP, "Metalloprotease is not essential for Vibrio vulnificus virulence in mice", Infect Immun, 68, pp.3569~3573 (2000)
- Kim YR, "Vibrio vulnificus RTX toxin kills host cells only after contact of the bacteria with host cells", Cell Microbiol, 10, pp.848~862 (2008)
- Kim CM, "Vibrio vulnificus metalloprotease VvpE is essentially required for swarming", FEMS Microbiol Lett, 269, pp.170~179 (2007)
- Kim CM, "Ferrophilic characteristics of Vibrio vulnificus and potential usefulness of iron chelation therapy", J Infect Dis, 195, pp.90~98 (2007)
- Kataoka E, "Application of highly polymorphic DNA markers to the identification of HeLa cell sublines", In Vitro Cell Dev Biol, 28A, pp.553~556 (1992)
