Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate
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Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for worm survival and reproduction. In Egypt, no available data were found about Wolbachia searching for it in either mosquitoes or filarial worms. Thus, we aimed to identify the possible concurrent presence of Wolbachia within different mosquitoes and filarial parasites, in Assiut Governorate, Egypt using multiplex PCR. Initially, 6 pools were detected positive for Wolbachia by single PCR. The simultaneous detection of Wolbachia and filarial parasites (Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens) by multiplex PCR was spotted in 5 out of 6 pools, with an overall estimated rate of infection (ERI) of 0.24%. Unexpectedly, the highest ERI (0.53%) was for Anopheles pharoensis with related Wolbachia and W. bancrofti, followed by Aedes (0.42%) and Culex (0.26%). We also observed that Wolbachia altered Culex spp. as a primary vector for W. bancrofti to be replaced by Anopheles sp. Wolbachia within filaria-infected mosquitoes in our locality gives a hope to use bacteria as a new control trend simultaneously targeting the vector and filarial parasites.
Abstract
Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for worm survival and reproduction. In Egypt, no available data were found about Wolbachia searching for it in either mosquitoes or filarial worms. Thus, we aimed to identify the possible concurrent presence of Wolbachia within different mosquitoes and filarial parasites, in Assiut Governorate, Egypt using multiplex PCR. Initially, 6 pools were detected positive for Wolbachia by single PCR. The simultaneous detection of Wolbachia and filarial parasites (Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens) by multiplex PCR was spotted in 5 out of 6 pools, with an overall estimated rate of infection (ERI) of 0.24%. Unexpectedly, the highest ERI (0.53%) was for Anopheles pharoensis with related Wolbachia and W. bancrofti, followed by Aedes (0.42%) and Culex (0.26%). We also observed that Wolbachia altered Culex spp. as a primary vector for W. bancrofti to be replaced by Anopheles sp. Wolbachia within filaria-infected mosquitoes in our locality gives a hope to use bacteria as a new control trend simultaneously targeting the vector and filarial parasites.
참고문헌 (54)
- Hertig M, "Studies on rickettsia-like microorganisms in insects", J Med Res, 44, pp.329~374 (1924)
- Iturbe-Ormaetxe I, "Wolbachia and the biological control of mosquito-borne disease", EMBO Rep, 12, pp.508~518 (2011)
- Werren JH, "Distribution of Wolbachia among neotropical arthropods", Proc Biol Sci, 262, pp.197~204 (1995)
- Kittayapong P, "Distribution and diversity of Wolbachia infections in Southeast Asian mosquitoes (Diptera: Culicidae)", J Med Entomol, 37, pp.340~345 (2000)
- Ricci I, "Searching for Wolbachia (Rickettsiales: Rickettsiaceae) in mosquitoes (Diptera: Culicidae): large polymerase chain reaction survey and new identifications", J Med Entomol, 39, pp.562~567 (2002)
- Rasgon JL, "An initial survey for Wolbachia (Rickettsiales: Rickettsiaceae) infection in selected California mosquitoes (Diptera: Culicidae)", J Med Entomol, 41, pp.255~257 (2004)
- Ravikumar H, "A preliminary survey for Wolbachia and bacteriophage WO infections in Indian mosquitoes (Diptera: Culicidae)", Trop Biomed, 27, pp.384~393 (2010)
- Bourtzis K, "Harnessing mosquito-Wolbachia symbiosis for vector and disease control", Acta Trop, 132(suppl), pp.S150~S163 (2014)
- Stouthamer R, "Wolbachia pipientis: microbial manipulator of arthropod reproduction", Annu Rev Microbiol, 53, pp.71~102 (1999)
- Turelli M, "Microbe-induced cytoplasmic incompatibility as a mechanism for introducing transgenes into arthropod populations", Insect Mol Biol, 8, pp.243~255 (1999)
- Pettigrew MM, "Control of vector-borne disease by genetic manipulation of insect vectors: technological requirements and research priorities", Austral J Entomol, 36, pp.309~317 (1997)
- Rasgon JL, "Wolbachia and cytoplasmic incompatibility in the California Culex pipiens mosquito species complex: parameter estimates and infection dynamics in natural populations", Genetics, 165, pp.2029~2038 (2003)
- Blagrove MS, "Wolbachia strain wMel induces cytoplasmic incompatibility and blocks dengue transmission in Aedes albopictus", Proc Natl Acad Sci U S A, 109, pp.255~260 (2012)
- Russell JA, "Specialization and geographic isolation among Wolbachia symbionts from ants and lycaenid butterflies", Evolution, 63, pp.624~640 (2009)
- Bandi C, "Phylogeny of Wolbachia in filarial nematodes", Proc Biol Sci, 265, pp.2407~2413 (1998)
- Taylor MJ, "16S rDNA phylogeny and ultrastructural characterization of Wolbachia intracellular bacteria of the filarial nematodes Brugia malayi, B. pahangi, and Wuchereria bancrofti", Exp Parasitol, 91, pp.356~361 (1999)
- Grandi G, "Wolbachia in Dirofilaria repens, an agent causing human subcutaneous dirofilariasis", J Parasitol, 94, pp.1421~1423 (2008)
- Slatko BE, "Wolbachia endosymbionts and human disease control", Mol Biochem Parasitol, 195, pp.88~95 (2014)
- Taylor MJ, "Wolbachia in the inflammatory pathogenesis of human filariasis", Ann N Y Acad Sci, 990, pp.444~449 (2003)
- Osei-Poku J, "Identification of Wolbachia strains in mosquito disease vectors", PLoS One, 7, pp.e49922~ (2012)
- "WHO Progress report 2000-2009 and strategic plan 2010-2020 of the global programme to eliminate lymphatic filariasis: halfway towards eliminating lymphatic filariasis" (2010)[URL]
- Latrofa MS, "Molecular xenomonitoring of Dirofilaria immitis and Dirofilaria repens in mosquitoes from north-eastern Italy by real-time PCR coupled with melting curve analysis", Parasit Vectors, 5, pp.76~83 (2012)
- Townson S, "Onchocerca parasites and Wolbachia endosymbionts: evaluation of a spectrum of antibiotic types for activity against Onchocerca gutturosa in vitro", Filaria J, 5, pp.1~9 (2006)
- Landum M, "Detection of Wolbachia in Dirofilaria infected dogs in Portugal", Vet Parasitol, 204, pp.407~410 (2014)
- Lee SF, "High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans", Appl Environ Microbiol, 78, pp.4740~4743 (2012)
- Taylor MJ, "Anti-Wolbachia drug discovery and development: safe macrofilaricides for onchocerciasis and lymphatic filariasis", Parasitology, 141, pp.119~127 (2014)
- Silver JB, "Mosquito ecology: field sampling methods" (2008), Springer
- Russell PF, "Keys to the Anopheline mosquitoes of the world with notes on their identification, distribution, biology, and relation to Malaria", pp.1~152 (1943), American Entomological Society
- Natvig LR, "Contributions to knowledge of the Danish and Fennoscandian mosquitoes-Culicini", Suppl Norsk Entomologisk Tidsskrift, pp.567~ (1948)
- Gad AM, "Mosquitoes of oasis of the Libyan Desert of Egypt", Bull Soc Entomol Egypt, 40, pp.131~136 (1963)
- Werren JH, "Wolbachia infection frequencies in insects: evidence of a global equilibrium?", Proc Biol Sci, 267, pp.1277~1285 (2000)
- Siridewa K, "Polymerase chain reaction-based technique for the detection of Wuchereria bancrofti in human blood samples, hydrocele fluid and mosquito vectors", Am J Trop Med Hyg, 54, pp.72~76 (1996)
- Rishniw M, "Discrimination between six species of canine microfilariae by a single polymerase chain reaction", Vet Parasitol, 135, pp.303~314 (2006)
- Cowling DW, "Comparison of methods for estimation of individual-level prevalence based on pooled samples", Prev Vet Med, 39, pp.211~225 (1999)
- Kassem HA, "Wolbachia infection and the expression of cytoplasmic incompatibility in sandflies (Diptera: Psychodidae) from Egypt", Ann Trop Med Parasitol, 97, pp.639~644 (2003)
- Rao RU, "A real-time PCR-based assay for detection of Wuchereria bancrofti and in blood and mosquitoes", Am J Trop Med Hyg, 74, pp.826~832 (2006)
- Mokhtar Y, "Parasitological Studies on Mosquitoes as Vectors for Parasitic Diseases with Special Reference to Filariae in Assiut Governorate", M.D., Parasitology Department, pp.2014
- Khalil GM, "A preliminary survey of mosquitoes in Upper Egypt", J Egypt Public Health Assoc, 55, pp.355~359 (1981)
- El-Nazer MM, "Parasitological and immunological studies on filarial worms in Assiut Governorate", Ph.D., Department of Medical Parasitology, pp.1990
- Duron O, "Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection, and recombination", Mol Ecol, 14, pp.1561~1573 (2005)
- Duron O, "Many compatible Wolbachia strains coexist within natural populations of Culex pipiens mosquito", Heredity, 106, pp.986~993 (2011)
- Cornel AJ, "Differences in extent of genetic introgression between sympatric Culex pipiens and Culex quinquefasciatus (Diptera: Culicidae) in California and South Africa", J Med Entomol, 40, pp.36~51 (2003)
- Rasgon JL, "Evolutionary history of a mosquito endosymbiont revealed through mitochondrial hitchhiking", Proc Biol Sci, 273, pp.1603~1611 (2006)
- Harb M, "The resurgence of lymphatic filariasis in the Nile Delta", Bull WHO, 71, pp.49~54 (1993)
- Farid H, "Selection of a strain of Culex pipiens highly susceptible to Wuchereria bancrofti", Egypt J Biol, 2, pp.125~131 (2000)
- Manguin S, "Review on global co-transmission of human Plasmodium species and Wuchereria bancrofti by Anopheles mosquitoes", Infect Genet Evol, 10, pp.159~177 (2010)
- Ahmed Kamal Dyab, "Xenomonitoring of Different Filarial Nematodes Using Single and Multiplex PCR in Mosquitoes from Assiut Governorate, Egypt", The Korean Journal of Parasitology, 53(1), pp.77~83 (2015), 대한기생충학ㆍ열대의학회
- Xi Z, "Generation of a novel Wolbachia infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection", Insect Biochem Mol Biol, 35, pp.903~910 (2005)
- Xi Z, "Wolbachia establishment and invasion in an Aedes aegypti laboratory population", Science, 310, pp.326~328 (2005)
- McMeniman CJ, "Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti", Science, 323, pp.141~144 (2009)
- Ramzy RM, "Field application of PCR-based assays for monitoring Wuchereria bancrofti infection in Africa", Ann Trop Med Parasitol, 96(suppl 2), pp.S55~S59 (2002)
- Bandi C, "Wolbachia in filarial nematodes: evolutionary aspects and implications for the pathogenesis and treatment of filarial diseases", Vet Parasitol, 98, pp.215~238 (2001)
- Pourali P, "PCR screening of the Wolbachia in some arthropods and nematodes in Khuzestan province", Iran J Vet Res, 10(3 ser. no. 28), pp.216~222 (2009), Shiraz University
- Simón F, "Human and animal dirofilariasis: the emergence of a zoonotic mosaic", Clin Microbiol Rev, 25, pp.507~544 (2012)
