본문 바로가기
KERD
논문KCI 등재

Antifilarial and Antibiotic Activities of Methanolic Extracts of Melaleuca cajuputi Flowers

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.

Abstract

We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.

참고문헌 (33)

  1. Michael E, "Re-assessing the global prevalence and distribution of lymphatic filariasis", Parasitology, 112, pp.409~428 (1996)
  2. Michael E, "Global mapping of lymphatic filariasis", Parasitol Today, 13, pp.472~476 (1997)
  3. Al-Abd NM, "Recent advances on the use of biochemical extracts as filaricidal agents", Evid Based Complementary Alternat Med, 2013, pp.986573~ (2013)
  4. Al-Abd NM, "Lymphatic filariasis in Peninsular Malaysia: a cross-sectional survey of the knowledge, attitudes, and practices of residents", Parasit Vectors, 7, pp.545~ (2014)
  5. Saini P, "Effect of ferulic acid from Hibiscus mutabilis on filarial parasite Setaria cervi: molecular and biochemical approaches", Parasitol Int, 61, pp.520~531 (2012)
  6. Kumari AK, "Issues in delivering morbidity management for lymphatic filariasis elimination: a study in Pondicherry, South India", ScientificWorldJournal, 2012, pp.372618~ (2012)
  7. Taylor MJ, "Parasitic Helminths: Targets, Screens, Drugs and Vaccines", pp.251~265 (2012), John Wiley & Sons
  8. Fernando SD, "Current evidence on the use of antifilarial agents in the management of bancroftian filariasis", J Trop Med, 2011, pp.175941~ (2011)
  9. Wolter F, "Piceatannol, a natural analog of resveratrol, inhibits progression through the S phase of the cell cycle in colorectal cancer cell lines", J Nutr, 132, pp.298~302 (2002)
  10. Kassim M, "Gelam honey inhibits lipopolysaccharide-induced endotoxemia in rats through the induction of heme oxygenase-1 and the inhibition of cytokines, nitric oxide, and high-mobility group protein B1", Fitoterapia, 83, pp.1054~1059 (2012)
  11. Funatogawa K, "Antibacterial activity of hydrolyzable tannins derived from medicinal plants against Helicobacter pylori", Microbiol Immunol, 48, pp.251~261 (2004)
  12. Liu J, "Pharmacology of oleanolic acid and ursolic acid", J Ethnopharmacol, 49, pp.57~68 (1995)
  13. Doran JC, "Tea Tree The Genus Melaleuca", pp.221~235 (1999), Halwood Academic Pulishers
  14. Hoerauf A, "Tetracycline therapy targets intracellular bacteria in the filarial nematode Litomosoides sigmodontis and results in filarial infertility", J Clin Invest, 103, pp.11~18 (1999)
  15. Hoerauf A, "Endosymbiotic bacteria in worms as targets for a novel chemotherapy in filariasis", Lancet, 355, pp.1242~1243 (2000)
  16. Ghedin E, "Brugia malayi gene expression in response to the targeting of the Wolbachia endosymbiont by tetracycline treatment", PLoS Negl Trop Dis, 3, pp.e525~ (2009)
  17. Bandi C, "Effects of tetracycline on the filarial worms Brugia pahangi and Dirofilaria immitis and their bacterial endosymbionts Wolbachia", Int J Parasitol, 29, pp.357~364 (1999)
  18. O'Neill SL, "16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects", Proc Natl Acad Sci USA, 89, pp.2699~2702 (1992)
  19. Zhou W, "Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences", Proc Biol Sci, 265, pp.509~515 (1998)
  20. Baldo L, "Multilocus sequence typing system for the endosymbiont Wolbachia pipientis", Appl Environ Microbiol, 72, pp.7098~7110 (2006)
  21. Gunawardena NK, "Differential effects of diethylcarbamazine, tetracycline and the combination on Brugia pahangi adult females in vitro", Parasitol Int, 54, pp.253~259 (2005)
  22. Rao R, "In vitro effects of antibiotics on Brugia malayi worm survival and reproduction", J Parasitol, 88, pp.605~611 (2002)
  23. Khunkitti W, "In vitro antifilarial activity of extracts of the medicinal plant Cardiospermum halicacabum against Brugia pahangi", J Helminthol, 74, pp.241~246 (2000)
  24. Mishra V, "Antifilarial activity of Azadirachta indica on cattle filarial parasite Setaria cervi", Fitoterapia, 76, pp.54~61 (2005)
  25. Sahare KN, "Antifilarial activity of ethyl acetate extract of Vitex negundo leaves in vitro", Asian Pac J Trop Med, 6, pp.689~692 (2013)
  26. Zaridah MZ, "In vitro antifilarial effects of three plant species against adult worms of subperiodic Brugia malayi", J Ethnopharmacol, 78, pp.79~84 (2001)
  27. Fujimaki Y, "Macrofilaricidal and microfilaricidal effects of Neurolaena lobata, a Guatemalan medicinal plant, on Brugia pahangi", J Helminthol, 79, pp.23~28 (2005)
  28. Sashidhara KV, "Galactolipids from Bauhinia racemosa as a new class of antifilarial agents against human lymphatic filarial parasite, Brugia malayi", Eur J Med Chem, 50, pp.230~235 (2012)
  29. Smith HL, "Tetracycline inhibits development of the infective-stage larvae of filarial nematodes in vitro", Exp Parasitol, 95, pp.265~270 (2000)
  30. Zhang X, "Analysis of Wolbachia strains associated with Conotrachelus nenuphar (Coleoptera: Curculionidae) in the Eastern United States", Environ Entomol, 39, pp.396~405 (2010)
  31. Rao RU, "Brugia malayi: effects of antibacterial agents on larval viability and development in vitro", Exp Parasitol, 101, pp.77~81 (2002)
  32. Langworthy NG, "Macrofilaricidal activity of tetracycline against the filarial nematode Onchocerca ochengi: elimination of Wolbachia precedes worm death and suggests a dependent relationship", Proc Biol Sci, 267, pp.1063~1069 (2000)
  33. Stolk WA, "Anti-Wolbachia treatment for lymphatic filariasis", Lancet, 365, pp.2067~2068 (2005)

관련 논문AI추천

Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora LeafThippayarat Chahomchuen, Wimonrut Insuan한국미생물·생명공학회 · 한국미생물·생명공학회지2020 · 제48권 제2호Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L.Ebrahim Saedi Dezaki, Fariba Sharififar1, Fatemeh Ezzatkhah, Hossein Mahmoudvand, Iraj Sharifi대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2015 · 제53권 제1호Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt GovernorateAbeer E. Mahmoud, Ahmed K. Dyab, Lamia A. Galal, Yasser Mokhtar대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2016 · 제54권 제3호Inhibition of Quorum Sensing Regulated Virulence Factors and Biofilm Formation by Eucalyptus globulus against Multidrug-Resistant Pseudomonas aeruginosaPankaj Kumar Sagar, Poonam Sharma, Rambir Singh대한약침학회 · Journal of Pharmacopuncture2022 · 제25권 제1호Antibacterial and Antibiofilm Properties of Kulim Bark (Scorodocarpus borneensis Becc.) Hydrogel on Polymicrobial BiofilmsDamayanti Ema, Murti Yosi Bayu, Pratiwi Sylvia Utami Tunjung, Sari Rafika대한약침학회 · Journal of Pharmacopuncture2025 · 제28권 제3호Antimicrobial activity of Garcinia mangostana L. ethanol extract against Cutibacterium acnes and Staphylococcus aureus국중기, 박순낭, 유소영, 임윤경대한구강생물학회 · International Journal of Oral Biology2019 · 제44권 제3호

이 간행물의 다른 논문 (20건)