Identifying Novel B Cell Epitopes within Toxoplasma gondii GRA6
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The study of antigenic epitopes from Toxoplasma gondii has not only enhanced our understanding of the structure and function of antigens, the reactions between antigens and antibodies, and many other aspects of immunology, but it also plays a significant role in the development of new diagnostic reagents and vaccines. In the present study, T. gondii GRA6 epitopes were identified using bioinformatics tools and a synthetic peptide technique. The potential B cell epitopes of GRA6 predicted by bioinformatics tools concentrated upon 3 regions of GRA6, 1-20 aa, 44-103 aa, and 172-221 aa. Ten shorter peptides from the 3 regions were synthesized and assessed by ELISA using pig sera from different time points after infection. Three of the 10 peptides (amino acids 44-63, 172-191, and 192-211) tested were recognized by all sera and determined to be immunodominant B-cell epitopes of GRA6. The results indicated that we precisely and accurately located the T. gondii GRA6 epitopes using pig sera collected at different time points after infection. The identified epitopes may be very useful for further studies of epitope-based vaccines and diagnostic reagents.
Abstract
The study of antigenic epitopes from Toxoplasma gondii has not only enhanced our understanding of the structure and function of antigens, the reactions between antigens and antibodies, and many other aspects of immunology, but it also plays a significant role in the development of new diagnostic reagents and vaccines. In the present study, T. gondii GRA6 epitopes were identified using bioinformatics tools and a synthetic peptide technique. The potential B cell epitopes of GRA6 predicted by bioinformatics tools concentrated upon 3 regions of GRA6, 1-20 aa, 44-103 aa, and 172-221 aa. Ten shorter peptides from the 3 regions were synthesized and assessed by ELISA using pig sera from different time points after infection. Three of the 10 peptides (amino acids 44-63, 172-191, and 192-211) tested were recognized by all sera and determined to be immunodominant B-cell epitopes of GRA6. The results indicated that we precisely and accurately located the T. gondii GRA6 epitopes using pig sera collected at different time points after infection. The identified epitopes may be very useful for further studies of epitope-based vaccines and diagnostic reagents.
참고문헌 (30)
- Dubey JP, "Persistence of immunity to toxoplasmosis in pigs vaccinated with a non-persistent strain of Toxoplasma gondii", Am J Vet Res, 55, pp.982~987 (1994)
- Ortega-Pacheco A, "Infection dynamic of Toxoplasma gondii in two fattening pig farms exposed to high and low cat density in an endemic region", Vet Parasitol, 175, pp.367~371 (2011)
- Wang YH, "Immunization with excreted-secreted antigens reduces tissue cyst formation in pigs", Parasitol Res, 112, pp.3835~3842 (2013)
- Cong H, "Multi-epitope DNA vaccine linked to the A2", B subunit of cholera toxin protect mice against Toxoplasma gondii, 2008, pp.26, 3913~3921
- Darcy F, "Protection of mice and nude rats against toxoplasmosis by a multiple antigenic peptide construction derived from Toxoplasma gondii P30 antigen", J Immunol, 149, pp.3636~3641 (1992)
- Godard I, "Antigenicity and immunogenicity of P30-derived peptides in experimental models of toxoplasmosis", Mol Immunol, 31, pp.1353~1363 (1994)
- Wang YH, "Increased survival time in mice vaccinated with a branched lysine multiple antigenic peptide containing B- and T-cell epitopes from T. gondii antigens", Vaccine, 29, pp.8619~8623 (2011)
- Dai JF, "Evaluation of a recombinant multiepitope peptide for serodiagnosis of Toxoplasma gondii infection", Clin Vaccine Immunol, 19, pp.338~342 (2012)
- Maksimov P, "Peptide microarray analysis of in silico-predicted epitopes for serological diagnosis of Toxoplasma gondii infection in humans", Clin Vaccine Immunol, 19, pp.865~874 (2012)
- Wang YH, "Screening and identification of novel B cell epitopes of Toxoplasma gondii SAG1", Parasit Vectors, 6, pp.125~ (2013)
- Wang YH, "Identification of novel B cell epitopes within Toxoplasma gondii GRA1", Exp Parasitol, 135, pp.606~610 (2013)
- Wang YH, "Analyzing and identifying novel B cell epitopes within Toxoplasma gondii GRA4", Parasit Vectors, 6, pp.125~ (2014)
- Golkar M, "Evaluation of protective effect of recombinant dense granule antigens GRA2 and GRA6 formulated in monophosphoryl lipid A(MPL)adjuvant against Toxoplasma chronic infection in mice", Vaccine, 25, pp.4301~4311 (2007)
- Lecordier L, "Enzyme-linked immunosorbent assays using the recombinant dense granule antigens GRA6 and GRA1 of Toxoplasma gondii for detection of immunoglobulin G antibodies", Clin Diagn Lab Immunol, 7, pp.607~611 (2000)
- Hiszczyńska-Sawicka E, "Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis", Acta Parasitol, 50, pp.249~254 (2005)
- Golkar M, "Serodiagnosis of recently acquired Toxoplasma gondii infection in pregnant women using enzyme-linked immunosorbent assays with a recombinant dense granule GRA6 protein", Diagn Microbiol Infect Dis, 61, pp.31~39 (2008)
- Elyasi H, "Use of dense granule antigen GRA6 in an immunoglobulin G avidity test to exclude acute Toxoplasma gondii infection during pregnancy", Clin Vaccine Immunol, 17, pp.1349~1355 (2010)
- Lecordier L1, "Characterization of a dense granule antigen of Toxoplasma gondii(GRA6)associated to the network of the parasitophorous vacuole", Mol Biochem Parasitol, 70, pp.85~94 (1995)
- Feliu V, "Location of the CD8 T cell epitope within the antigenic precursor determines immunogenicity and protection against the Toxoplasma gondii parasite", PLoS Pathog, 9, pp.e1003449~ (2013)
- Garnier J, "Prediction of protein structure and the principles of protein conformation", pp.417~465 (1989), Plenum Press
- Chou PY, "Prediction of the secondary structure of proteins from their amino acid sequence", Adv Enzymol, 47, pp.45~148 (1987)
- Kyte J, "A simple method for displaying the hydropathic character of a protein", J Mol Biol, 157, pp.105~132 (1982)
- Karplus PA, "Prediction of chain flexibility in proteins: a tool for the selection of peptide antigens", Naturwissenschaften, 72, pp.212~213 (1985)
- Emini EA, "Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide", J Virol, 55, pp.836~839 (1985)
- Jameson BA, "The antigenic index: a novel algorithm for predicting antigenic determinants", Comput Appl Biosci, 181, pp.212~213 (1988)
- Cardona N, "Gomez-Marin. Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis", Exp Parasitol, 123, pp.199~202 (2009)
- Zhang ZW, "Screening and identification of B cell epitopes of structural proteins of foot-and-mouth disease virus serotype Asia I", Vet Microbiol, 140, pp.25~33 (2010)
- Frank R, "The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports-principles and applications", J Immunol Methods, 267, pp.13~26 (2002)
- Siachoque H, "Toxoplasma gondii: Immunogenicity and protection by P30 peptides in a murine model", Exp Parasitol, 114, pp.62~65 (2006)
- Vita R, "The immune epitope database 2.0", Nucleic Acids Res, 38, pp.D854~D862 (2010)
