Serodiagnosis of Extraintestinal Amebiasis: Retrospective Evaluation of the Diagnostic Performance of the Bordier® ELISA Kit
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Soluble antigens from an axenic culture of Entamoeba histolytica were used to develop a commercial ELISA kit to quantify anti-E. histolytica antibodies in sera of patients with extraintestinal amebiasis in non-endemic settings. The diagnostic specificity and sensitivity of the test were assessed retrospectively using 131 human serum samples with amoebic serologic status available. They were selected according to their results in immunofluorescence (IFAT) and were separated in 2 sample categories: 64 sera with positive results by IFAT and 67 with negative results by IFAT. The sensitivity and specificity of the ELISA kit were assessed at 95.0% and 94.0% compared to the IFAT. The test can be useful to exclude a potential diagnosis of amebiasis and could be used as a screening method since ELISA is an automated technique.
Abstract
Soluble antigens from an axenic culture of Entamoeba histolytica were used to develop a commercial ELISA kit to quantify anti-E. histolytica antibodies in sera of patients with extraintestinal amebiasis in non-endemic settings. The diagnostic specificity and sensitivity of the test were assessed retrospectively using 131 human serum samples with amoebic serologic status available. They were selected according to their results in immunofluorescence (IFAT) and were separated in 2 sample categories: 64 sera with positive results by IFAT and 67 with negative results by IFAT. The sensitivity and specificity of the ELISA kit were assessed at 95.0% and 94.0% compared to the IFAT. The test can be useful to exclude a potential diagnosis of amebiasis and could be used as a screening method since ELISA is an automated technique.
참고문헌 (16)
- Tanyuksel M, "Laboratory diagnosis of amebiasis", Clin Microbiol Rev, 16, pp.713~729 (2003)[DOI]
- Ali IK, "Molecular epidemiology of amebiasis", Infect Genet Evol, 8, pp.698~707 (2008)[DOI]
- Mortimer L, "The immunopathogenesis of Entamoeba histolytica", Exp Parasitol, 126, pp.366~380 (2010)[DOI]
- Kimura K, "Amebiasis: modern diagnostic imaging with pathological and clinical correlation", Semin Roentgenol, 32, pp.250~275 (1997)[DOI]
- Merens A, "Utility and limitations of laboratory diagnosis of amebiasis", Med Trop, 65, pp.167~175 (2005)
- Ralston KS, "Tissue destruction and invasion by Entamoeba histolytica", Trends Parasitol, 27, pp.254~263 (2011)[DOI]
- Stanley SL Jr, "Amebiasis", Lancet, 361, pp.1025~1034 (2003)[DOI]
- Petri WA Jr, "Diagnosis and management of amebiasis", Clin Infect Dis, 29, pp.1117~1125 (1999)[DOI]
- "WHO", PAHO, Epidemiol Bull, pp.1997, 18[DOI]
- Tan ZN, "Efficacies of two in-house indirect ELISAs for the diagnosis of amoebic liver abscess", Trop Biomed, 30, pp.250~256 (2013)
- Larreche S, "Effectiveness of latex agglutination slide test in the diagnosis of improved invasive amoebiasis in the emergency department", Diagn Microbiol Infect Dis, 77, pp.335~336 (2013)[DOI]
- Ning TZ, "Evaluation of Entamoeba histolytica recombinant phosphoglucomutase protein for serodiagnosis of amoebic liver abscess", BMC Infect Dis, 13, pp.144~ (2013)[DOI]
- Wong WK, "Analysis of Entamoeba histolytica excretorysecretory antigen and identification of a new potential diagnostic marker", Clin Vaccine Immunol, 18, pp.1913~1917 (2011)[DOI]
- Wong WK, "Parallel ELISAs using crude soluble antigen and excretory-secretory antigen for improved serodiagnosis of amoebic liver abscess", Acta Trop, 172, pp.208~212 (2017)[DOI]
- 2.0.CO;2,
- Yamaura H, "Evaluation of dot-ELISA for serological diagnosis of amebiasis", J Infect Chemother, 9, pp.25~29 (2003)[DOI]