Identification of Free-Living Amoebas in Tap Water of Buildings with Storage Tanks in Korea
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초록
Free-living amoebas (FLAs) can cause severe disease in humans and animals when they become infected. However, there are no accurate survey reports on the prevalence of FLAs in Korea. In this study, we collected 163 tap water samples from buildings, apartments, and restrooms of highway service areas in 7 Korean provinces with high population density. All these buildings and facilities have water storage tanks in common. The survey was separated into categories of buildings, apartments, and highway service areas. Five hundred milliliters of tap water from each building was collected and filtered with 0.2 μm pore filter paper. The filters were incubated in agar plates with heated E. coli at 25°C. After axenization, genomic DNA was collected from each FLA, and species classification was performed using partial 18S-rDNA PCR-sequencing analysis. We found that 12.9% of tap water from buildings with storage tanks in Korea was contaminated with FLAs. The highway service areas had the highest contamination rate at 33.3%. All of the FLAs, except one, were genetically similar to Vermamoeba vermiformis (Hartmannella vermiformis). The remaining FLA (KFA21) was very similar to Acanthamoeba lugdunensis (KA/E26). Although cases of human infection by V. vermiformis are very rare, we must pay attention to the fact that one-third of tap water supplies in highway service areas have been contaminated.
Abstract
Free-living amoebas (FLAs) can cause severe disease in humans and animals when they become infected. However, there are no accurate survey reports on the prevalence of FLAs in Korea. In this study, we collected 163 tap water samples from buildings, apartments, and restrooms of highway service areas in 7 Korean provinces with high population density. All these buildings and facilities have water storage tanks in common. The survey was separated into categories of buildings, apartments, and highway service areas. Five hundred milliliters of tap water from each building was collected and filtered with 0.2 μm pore filter paper. The filters were incubated in agar plates with heated E. coli at 25°C. After axenization, genomic DNA was collected from each FLA, and species classification was performed using partial 18S-rDNA PCR-sequencing analysis. We found that 12.9% of tap water from buildings with storage tanks in Korea was contaminated with FLAs. The highway service areas had the highest contamination rate at 33.3%. All of the FLAs, except one, were genetically similar to Vermamoeba vermiformis (Hartmannella vermiformis). The remaining FLA (KFA21) was very similar to Acanthamoeba lugdunensis (KA/E26). Although cases of human infection by V. vermiformis are very rare, we must pay attention to the fact that one-third of tap water supplies in highway service areas have been contaminated.
참고문헌 (26)
- Greub G, "Microorganisms resistant to free-living amoebae", Clin Microbiol Rev, 17, pp.413~433 (2004)
- Schuster FL, "Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals", Int J Parasitol, 34, pp.1001~1027 (2004)
- Martinez AJ, "Free-Living Amoebas: natural history, prevention, diagnosis, pathology and treatment of disease" (1985), CRC Press
- Aitken D, "Amebic keratitis in a wearer of disposable contact lenses due to a mixed Vahlkampfia and Hartmannella infection", Ophthalmology, 103, pp.485~494 (1996)
- Grace E, "Naegleria fowleri : pathogenesis, diagnosis, and treatment options", Antimicrob Agents Chemother, 59, pp.6677~6681 (2015)
- Niyyati M, "Isolation of Balamuthia mandrillaris from urban dust, free of known infectious involvement", Parasitol Res, 106, pp.279~281 (2009)
- Qvarnstrom Y, "Molecular confirmation of Sappinia pedata as a causative agent of amoebic encephalitis", J Infect Dis, 199, pp.1139~1142 (2009)
- Stehr-Green JK, "The epidemiology of Acanthamoeba keratitis in the United States", Am J Ophthalmol, 107, pp.331~336 (1989)
- Park MK, "Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2", PLoS One, 9, pp.e92726~ (2014)
- National Research Council, "Drinking Water and Health, Volume 4" (1982), The National Academies Press
- Kilvington S, "Acanthamoeba keratitis : the role of domestic tap water contamination in the United Kingdom", Invest Ophthalmol Vis Sci, 45, pp.165~169 (2004)
- Marciano-Cabral F, "Free-living amoebae, Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA", J Water Health, 8, pp.71~82 (2010)
- 이선주, "Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear", Parasites, Hosts and Diseases, 44(4), pp.313~320 (2006), 대한기생충학ㆍ열대의학회
- Visvesvara GS, "Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea", FEMS Immunol Med Microbiol, 50, pp.1~26 (2007)
- Kang H, "Molecular detection of free-living amoebae from Namhangang(southern Han River)in Korea", Sci Rep, 10, pp.335~ (2020)
- Corliss JO, "Comments on the role of the cyst in the life cycle and survival of free-living protozoa", Trans Am Microsc Soc, 93, pp.578~593 (1974)
- Trabelsi H, "Pathogenic free-living amoebae : epidemiology and clinical review", Pathol Biol (Paris), 60, pp.399~405 (2012)
- Castrillón JC, "Acanthamoeba spp. as opportunistic pathogens parasites", Rev Chilena Infectol, 30, pp.147~155 (2013)
- Carnt NP, "Acanthamoeba keratitis : confirmation of the UK outbreak and a prospective case-control study identifying contributing risk factors", Br J Ophthalmol, 102, pp.1621~1628 (2018)
- 정해진, "The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea", Parasites, Hosts and Diseases, 43(2), pp.1~13 (2005), 대한기생충학ㆍ열대의학회
- Kong HH, "Mitochondrial DNA restriction fragment length polymorphism(RFLP)and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea", J Clin Microbiol, 40, pp.1199~1206 (2002)
- Yu HS, "Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea", Korean J Parasitol, 39, pp.161~170 (2001)
- Yu HS, "Laboratory investigation of Acanthamoeba lugdunensis from patients with keratitis", Invest Ophthalmol Vis Sci, 45, pp.1418~1426 (2004)
- Schaumberg DA, "The epidemic of Acanthamoeba keratitis : where do we stand", Cornea, 17, pp.3~10 (1998)
- Rodríguez-Zaragoza S, "Ecology of free-living amoebae", Crit Rev Microbiol, 20, pp.225~241 (1994)
- Scheid PL, "Vermamoeba vermiformis as etiological agent of a painful ulcer close to the eye", Parasitol Res, 118, pp.1999~2004 (2019)