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Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) from Riparian People along the Mekong River in Cambodia

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초록

Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) is described based on adult flukes collected from humans residing along the Mekong River in Cambodia. Total 256 flukes were collected from the diarrheic stool of 6 echinostome egg positive villagers in Kratie and Takeo Province after praziquantel treatment and purging. Adults of the new species were 9.0–13.1 (av. 11.3) mm in length and 1.3–2.5 (1.9) mm in maximum width and characterized by having a head collar armed with 37 collar spines (dorsal spines arranged in 2 alternative rows), including 5 end group spines. The eggs in feces and worm uterus were 98–132 (117) μm long and 62–90 (75) μm wide. These morphological features closely resembled those of Echinostoma revolutum, E. miyagawai, and several other 37-collar-spined Echinostoma species. However, sequencing of the nuclear ITS (ITS1-5.8S rRNA-ITS2) and 2 mitochondrial genes, cox1 and nad1, revealed unique features distinct from E. revolutum and also from other 37-collar-spined Echinostoma group available in GenBank (E. bolschewense, E. caproni, E. cinetorchis, E. deserticum, E. miyagawai, E. nasincovae, E. novaezealandense, E. paraensei, E. paraulum, E. robustum, E. trivolvis, and Echinostoma sp. IG). Thus, we assigned our flukes as a new species, E. mekongi. The new species revealed marked variation in the morphology of testes (globular or lobulated), and smaller head collar, collar spines, oral and ventral suckers, and cirrus sac compared to E. revolutum and E. miyagawai. Epidemiological studies regarding the geographical distribution and its life history, including the source of human infections, remain to be performed.

Abstract

Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) is described based on adult flukes collected from humans residing along the Mekong River in Cambodia. Total 256 flukes were collected from the diarrheic stool of 6 echinostome egg positive villagers in Kratie and Takeo Province after praziquantel treatment and purging. Adults of the new species were 9.0–13.1 (av. 11.3) mm in length and 1.3–2.5 (1.9) mm in maximum width and characterized by having a head collar armed with 37 collar spines (dorsal spines arranged in 2 alternative rows), including 5 end group spines. The eggs in feces and worm uterus were 98–132 (117) μm long and 62–90 (75) μm wide. These morphological features closely resembled those of Echinostoma revolutum, E. miyagawai, and several other 37-collar-spined Echinostoma species. However, sequencing of the nuclear ITS (ITS1-5.8S rRNA-ITS2) and 2 mitochondrial genes, cox1 and nad1, revealed unique features distinct from E. revolutum and also from other 37-collar-spined Echinostoma group available in GenBank (E. bolschewense, E. caproni, E. cinetorchis, E. deserticum, E. miyagawai, E. nasincovae, E. novaezealandense, E. paraensei, E. paraulum, E. robustum, E. trivolvis, and Echinostoma sp. IG). Thus, we assigned our flukes as a new species, E. mekongi. The new species revealed marked variation in the morphology of testes (globular or lobulated), and smaller head collar, collar spines, oral and ventral suckers, and cirrus sac compared to E. revolutum and E. miyagawai. Epidemiological studies regarding the geographical distribution and its life history, including the source of human infections, remain to be performed.

참고문헌 (40)

  1. 채종일, "Taxonomy of Echinostoma revolutum and 37-Collar-Spined Echinostoma spp.: A Historical Review", Parasites, Hosts and Diseases, 58(4), pp.343~371 (2020), 대한기생충학ㆍ열대의학회[DOI]
  2. Chai JY, "Human Intestinal Flukes. Chapter 2. Echinostomes", pp.169~343 (2019), Springer Nature B.V
  3. Chai JY, "The Biology of Echinostomes", pp.147~183 (2009), Springer
  4. Kanev I, "Life-cycle, delimitation and redescription of Echinostoma revolutum(Froelich, 1802)(Trematoda : Echinostomatidae)", Syst Parasitol, 28, pp.125~144 (1994)
  5. Nagataki M, "Mitochondrial DNA sequences of 37 collar-spined echinostomes (Digenea: Echinostomatidae) in Thailand and Lao PDR reveals presence of two species: Echinostoma revolutum and E. miyagawai", Infect Genet Evol, 35, pp.56~62 (2015)
  6. Georgieva S, "Molecular characterization of four echinostomes (Digenea: Echinostomatidae) from birds in New Zealand, with descriptions of Echinostome novaezealandense n. sp. and Echinoparyphium poulini n. sp", Syst Parasitol, 94, pp.477~497 (2017)
  7. Kostadinova A, "Phylogenetic relationships of Echinostoma Rudolphi, 1809(Digenea : Echinostomatidae)and related genera re-assessed via DNA and morphological analyses", Syst Parasitol, 54, pp.159~176 (2003)
  8. Georgieva S, "New cryptic species of the ‘revolutum’ group of Echinostoma(Digenea : Echinostomatidae)revealed by molecular and morphological data", Parasit Vectors, 6, pp.64~ (2013)
  9. Georgieva S, "Echinostoma ‘revolutum’(Digenea : Echinostomatidae)species complex revisited : species delimitation based on novel molecular and morphological data gathered in Europe", Parasit Vectors, 7, pp.520~ (2014)
  10. Detwiler JT, "Revealing the secret lives of cryptic species : examining the phylogenetic relationships of echinostome parasites in North America", Mol Phylogenet Evol, 55, pp.611~620 (2010)
  11. Detwiler JT, "Revealing cryptic parasite diversity in a definitive host : echinostomes in muskrats", J Parasitol, 98, pp.1148~1155 (2012)
  12. Morgan JA, "Mitochondrial ND1 gene sequences used to identify echinostome isolates from Australia and New Zealand", Int J Parasitol, 28, pp.493~502 (1998)
  13. Saijuntha W, "Genetic variation and relationships of four species of medically important echinostomes(Trematoda : Echinostomatidae)in South-East Asia", Infect Genet Evol, 11, pp.375~381 (2011)
  14. Noikong W, "Molecular analysis of echinostome metacercariae from their second intermediate host found in a localised geographic region reveals genetic heterogeneity and possible cryptic speciation", PLoS Negl Trop Dis, 8, pp.e2778~ (2014)
  15. Mohanta UK, "Characterization of Echinostoma revolutum and Echinostoma robustum from ducks in Bangladesh based on morphology, nuclear ribosomal ITS2 and mitochondrial nad1 sequences", Parasitol Int, 69, pp.1~7 (2019)
  16. Morgan JA, "Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species(Trematoda)", Parasitology, 116, pp.289~297 (1998)
  17. 채종일, "Echinostome Flukes Receovered from Humans in Khammouane Province, Lao PDR", Parasites, Hosts and Diseases, 50(3), pp.269~272 (2012), 대한기생충학ㆍ열대의학회
  18. Faltýnková A, "A re-assessment of species diversity within the ‘revolutum’ group of Echinostoma Rudolphi, 1809(Digenea : Echinostomatidae)in Europe", Syst Parasitol, 90, pp.1~25 (2015)
  19. Luton K, "Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes(Platyhelminthes : Trematoda : Digenea)", Mol Biochem Parasitol, 56, pp.323~328 (1992)
  20. Tamura K, "MEGA6: Molecular Evolutionary Genetics Analysis version 6.0", Mol Biol Evol, 30, pp.2725~2729 (2013)
  21. Yamaguti S, "Studies on the helminth fauna of Japan. Part 3. Avian trematodes, II", Jpn J Zool, 5, pp.542~583 (1934)
  22. Yamaguti S, "Studies on the helminth fauna of Japan. Part 5. Trematodes of birds, III", Jpn J Zool, 6, pp.159~182 (1935)
  23. Sandground JH, "Echinostoma lindoensis n. sp., a new parasite of man in the Celebes with an account of its life history and epidemiology", Am J Trop Med Hyg, 20, pp.511~536 (1940)
  24. Kanev I, "Redescription of Echinostoma jurini (Skvortzov, 1924) with a discussion of its identity and characteristics", Ann Naturhist Mus Wien, 97, pp.37~53 (1995)
  25. Reddy DB, "Artyfechinostomum mehrai infestation in man", J Trop Med Hyg, 67, pp.58~59 (1964)
  26. 손운목, "Echinostoma ilocanum Infection in Oddar Meanchey Province, Cambodia", Parasites, Hosts and Diseases, 49(2), pp.187~190 (2011), 대한기생충학ㆍ열대의학회
  27. Sohn WM, "Echinostoma revolutum infection in children, Pursat Province, Cambodia", Emerg Infect Dis, 17, pp.117~119 (2011)
  28. Anh NTL, "Animal reservoir hosts and fish-borne zoonotic trematode infections on fish farms, Vietnam", Emerg Infect Dis, 15, pp.540~546 (2009)
  29. Anh NTL, "Poultry as reservoir hosts for fishborne zoonotic trematodes in Vietnamese fish farms", Vet Parasitol, 169, pp.391~394 (2010)
  30. 채종일, "Echinostoma revolutum: Metacercariae in Filopaludina Snails from Nam Dinh Province, Vietnam, and Adults from Experimental Hamsters", Parasites, Hosts and Diseases, 49(4), pp.449~455 (2011), 대한기생충학ㆍ열대의학회
  31. Sayasone S, "Rare human infection with the trematode Echinochasmus japonicus in Lao PDR", Parasitol Int, 58, pp.106~109 (2009)
  32. 손운목, "Echinostoma macrorchis in Lao PDR: Metacercariae in Cipangopaludina Snails and Adults from Experimentally Infected Animals", Parasites, Hosts and Diseases, 51(2), pp.191~196 (2013), 대한기생충학ㆍ열대의학회
  33. 채종일, "Echinostoma ilocanum Infection in Two Residents of Savannakhet Province, Lao PDR", Parasites, Hosts and Diseases, 56(1), pp.75~79 (2018), 대한기생충학ㆍ열대의학회
  34. 채종일, "Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR", Parasites, Hosts and Diseases, 58(1), pp.67~72 (2020), 대한기생충학ㆍ열대의학회[DOI]
  35. Jong-Yil Chai, "Echinochasmus caninus n. comb. (Trematoda: Echinostomatidae) Infection in Eleven Riparian People in Khammouane Province, Lao PDR", Parasites, Hosts and Diseases, 57(4), pp.451~456 (2019), 대한기생충학ㆍ열대의학회[DOI]
  36. Bhaibulaya M, "The finding of Echinostoma revolutum infection in man in Thailand", J Med Assoc Thailand, 49, pp.83~ (1966)
  37. Radomyos P, "Multi-infection with helminths in adults from northeast Thailand as determined by post-treatment fecal examination of adult worms", Trop Med Parasitol, 45, pp.133~135 (1994)
  38. Saijuntha W, "Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum(Trematoda : Echinostomatidae)in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence", Parasitol Res, 108, pp.751~755 (2011)
  39. Buddhachat K, "Is species identification of Echinostoma revolutum using mitochondrial DNA barcoding feasible with high-resolution melting analysis?", Parasitol Res, 118, pp.1799~1810 (2019)
  40. World Health Organization, "Basic Laboratory Methods in Medical Parasitology", World Health Organization, 26~ (1991)

관련 논문AI추천

Echinostoma mekongi: Discovery of Its Metacercarial Stage in Snails, Filopaludina martensi cambodjensis, in Pursat Province, Cambodia손운목, 이건훈, 장태희, 정봉광, 조재은, 채종일, Rekol Huy, Virak Khieu대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2021 · 제59권 제1호Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR손운목, 신혜주, 용태순, 임한종, 장태희, 정봉광, 채종일, Bounlay Phammasack, Bounnaloth Insisiengmay, Duk-Young Min, Keeseon S. Eom대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2020 · 제58권 제1호Echinochasmus caninus n. comb. (Trematoda: Echinostomatidae) Infection in Eleven Riparian People in Khammouane Province, Lao PDR손운목, 신혜주, 용태순, 임한종, 장태희, 정봉광, Bounlay Phammasac, Bounnaloth Insisiengmay, Duk-Young Min, Jong-Yil Chai, Keeseon S. Eom대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2019 · 제57권 제4호Echinostome Flukes Receovered from Humans in Khammouane Province, Lao PDR민득영, 손운목, 엄기선, 용태순, 임한종, 채종일, Bounlay Phammasack, Bounnaloth Insisiengmay, Eui-Hyug Hoang대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2012 · 제50권 제3호Echinostoma ilocanum Infection in Oddar Meanchey Province, Cambodia손운목, 엄기선, 용태순, 채종일, A-Reum Kang, Duong Socheat, Hoo-Gn Jeong, Hyeong-Jin Kim, Jae-Kwang Kim, Jung-Mi Park, Mok-Ryun Kim, Muth Sinuon, Soo-Hyeon Ji대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2011 · 제49권 제2호Echinostoma macrorchis Metacercariae in Cipangopaludina chinensis malleata from Xiengkhuang Province, Lao PDR and Morphologies of Adults from Experimental Animals나병국, 손운목, 용태순, 채종일, DongMinLee, Duk-Young Min, Keeseon S. Eom대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2019 · 제57권 제6호

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