Echinostoma mekongi: Discovery of Its Metacercarial Stage in Snails, Filopaludina martensi cambodjensis, in Pursat Province, Cambodia
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Echinostoma mekongi was reported as a new species in 2020 based on specimens collected from humans in Kratie and Takeo Province, Cambodia. In the present study, its metacercarial stage has been discovered in Filopaludina martensi cambodjensis snails purchased from a local market nearby the Tonle Sap Lake, Pursat Province, Cambodia. The metacercariae were fed orally to an experimental hamster, and adult flukes were recovered at day 20 post-infection. They were morphologically examined using light and scanning electron microscopes and molecularly analyzed by sequencing of their mitochondrial cox1 and nad1 genes. A total of 115 metacercariae (1-8 per snail) were detected in 60 (60.0%) out of 100 Filopaludina snails examined. The metacercariae were round, 174 μm in average diameter (163-190 μm in range), having a thin cyst wall, a head collar armed with 37 collar spines, and characteristic excretory granules. The adult flukes were elongated, ventrally curved, 7.3 (6.4-8.2)×1.4 (1.1-1.7) mm in size, and equipped with 37 collar spines on the head collar (dorsal spines in 2 alternating rows), being consistent with E. mekongi. In phylogenetic analyses, the adult flukes showed 99.0-100% homology based on cox1 sequences and 98.9-99.7% homology based on nad1 sequences with E. mekongi. The results evidenced that F. martensi cambodjensis snails act as the second intermediate host of E. mekongi, and hamsters can be used as a suitable experimental definitive host. As local people favor to eat undercooked snails, these snails seem to be an important source of human infection with E. mekongi in Cambodia.
Abstract
Echinostoma mekongi was reported as a new species in 2020 based on specimens collected from humans in Kratie and Takeo Province, Cambodia. In the present study, its metacercarial stage has been discovered in Filopaludina martensi cambodjensis snails purchased from a local market nearby the Tonle Sap Lake, Pursat Province, Cambodia. The metacercariae were fed orally to an experimental hamster, and adult flukes were recovered at day 20 post-infection. They were morphologically examined using light and scanning electron microscopes and molecularly analyzed by sequencing of their mitochondrial cox1 and nad1 genes. A total of 115 metacercariae (1-8 per snail) were detected in 60 (60.0%) out of 100 Filopaludina snails examined. The metacercariae were round, 174 μm in average diameter (163-190 μm in range), having a thin cyst wall, a head collar armed with 37 collar spines, and characteristic excretory granules. The adult flukes were elongated, ventrally curved, 7.3 (6.4-8.2)×1.4 (1.1-1.7) mm in size, and equipped with 37 collar spines on the head collar (dorsal spines in 2 alternating rows), being consistent with E. mekongi. In phylogenetic analyses, the adult flukes showed 99.0-100% homology based on cox1 sequences and 98.9-99.7% homology based on nad1 sequences with E. mekongi. The results evidenced that F. martensi cambodjensis snails act as the second intermediate host of E. mekongi, and hamsters can be used as a suitable experimental definitive host. As local people favor to eat undercooked snails, these snails seem to be an important source of human infection with E. mekongi in Cambodia.
참고문헌 (18)
- 채종일, "Taxonomy of Echinostoma revolutum and 37-Collar-Spined Echinostoma spp.: A Historical Review", The Korean Journal of Parasitology, 58(4), pp.343~371 (2020), 대한기생충학ㆍ열대의학회[DOI]
- 채종일, "Echinostoma miyagawai Ishii, 1932 (Echinostomatidae) from Ducks in Aceh Province, Indonesia with Special Reference to Its Synonymy with Echinostoma robustum Yamaguti, 1935", The Korean Journal of Parasitology, 59(1), pp.35~45 (2021), 대한기생충학ㆍ열대의학회
- 조재은, "Echinostoma mekongi n. sp. (Digenea: Echinostomatidae) from Riparian People along the Mekong River in Cambodia", The Korean Journal of Parasitology, 58(4), pp.431~443 (2020), 대한기생충학ㆍ열대의학회[DOI]
- Ng TH, "Annotated checklist of freshwater mollusk from the largest freshwater lake in Southeast Asia", ZooKeys, 958, pp.107~141 (2020)[DOI]
- 채종일, "Larval Gnathostomes and Spargana in Chinese Edible Frogs, Hoplobatrachus rugulosus, from Myanmar: Potential Risk of Human Infection", The Korean Journal of Parasitology, 58(4), pp.467~473 (2020), 대한기생충학ㆍ열대의학회[DOI]
- Morgan JA, "Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda)", Parasitology, 116, pp.289~297 (1998)[DOI]
- Larkin MA, "Clustal W and Clustal X version 2.0", Bioinformatics, pp.2947~2948 (2007)[DOI]
- Kumar S, "MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms", Mol Biol Evol, 35, pp.1547~1549 (2018)[DOI]
- 채종일, "Echinostoma revolutum: Metacercariae in Filopaludina Snails from Nam Dinh Province, Vietnam, and Adults from Experimental Hamsters", The Korean Journal of Parasitology, 49(4), pp.449~455 (2011), 대한기생충학ㆍ열대의학회
- Kittichai Chantima, "Echinostoma revolutum: Freshwater Snails as the Second Intermediate Hosts in Chiang Mai, Thailand", The Korean Journal of Parasitology, 51(2), pp.183~189 (2013), 대한기생충학ㆍ열대의학회
- Noikong W, "Molecular analysis of echinostome metacercariae from their second intermediate host found in a localised geographic region reveals genetic heterogeneity and possible cryptic speciation", PLoS Negl Trop Dis, 8, pp.e2778~ (2014)[DOI]
- Wang YC, "Assessing the role of Filopaludina martensi martensi as a biocontrol agent of Bithynia siamensis goniomphalos, the first intermediate host of Opisthorchis viverrini", Parasitol Res, 119, pp.3415~3431 (2020)[DOI]
- Chai JY, "Human Intestinal Fluke:From Discovery to Treatment and Control", pp.1~549 (2019), Springer Nature
- Kostadinova A, "Re-validation of Echinostoma miyagawai Ishii, 1932 (Digenea: Echinostomatidae) on the basis of the experimental completion of its life-cycle", Syst Parasitol, 45, pp.81~108 (2000)[DOI]
- Faltýnková A, "A re-assessment of species diversity within the ‘revolutum’ group of Echinostoma Rudolphi, 1809 (Digenea: Echinostomatidae) in Europe", Syst Parasitol, 90, pp.1~25 (2015)[DOI]
- Kanev I, "Life-cycle, delimitation and redescription of Echinostoma revolutum (Froelich, 1802) (Trematoda: Echinostomatidae)", Syst Parasitol, 28, pp.125~144 (1994)[DOI]
- Sandground JH, "Echinostoma lindoensis n. sp., a new parasite of man in the Celebes with an account of its life history and epidemiology", Am J Trop Med Hyg, 20, pp.511~536[DOI]
- Sohn WM, "Echinostoma revolutum infection in children, Pursat Province, Cambodia", Emerg Infect Dis, 17, pp.117~119 (2011)[DOI]