동해안 사구로부터 Auxin을 생산하는 Bacillus cereus A‐139의 분리 및 그 특성
Isolation and Characterization of Bacillus cereus A‐139 Producing Auxin from East Coast Sand Dunes
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A bacterium, which was named to be Bacillus cereus A‐139, secreting auxin was isolated from the East Coast Sand Dunes in Korea. The secretion of auxin was confirmed by HPLC. When cultured in LB broth, Bacillus cereus A‐139 produced 16.12 µg/mL auxin after 8 days in LB broth. Bacillus cereus A‐139 produced 49 µg/mL auxin and 162.6 µg/mL by the addition of 2% tryptone and 0.1% tryptophan, respectively. The root growth of Arabidopsis thaliana was retarded by Bacillus cereus A‐139 culture broth up to 57% but the formation of lateral roots was increased up to almost 2 times after 4 days incubation. Also the formation of lateral roots of mung bean was increased up to 57% after 10 days incubation.
Abstract
A bacterium, which was named to be Bacillus cereus A‐139, secreting auxin was isolated from the East Coast Sand Dunes in Korea. The secretion of auxin was confirmed by HPLC. When cultured in LB broth, Bacillus cereus A‐139 produced 16.12 µg/mL auxin after 8 days in LB broth. Bacillus cereus A‐139 produced 49 µg/mL auxin and 162.6 µg/mL by the addition of 2% tryptone and 0.1% tryptophan, respectively. The root growth of Arabidopsis thaliana was retarded by Bacillus cereus A‐139 culture broth up to 57% but the formation of lateral roots was increased up to almost 2 times after 4 days incubation. Also the formation of lateral roots of mung bean was increased up to 57% after 10 days incubation.
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