Discorea alata L.의 항균 및 항산화 활성
Antimicrobial and Antioxidant Activity of the Discorea alata L..
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본 연구에서는 최근 국내재배가 시작한 D. alata L. 경북6호의 성분 및 색차를 D. batatas 경북 1호와 비교 분석하였으며, 메탄올 추출물 및 이의 분획물의 항산화 및 항균력을비교 평가하였다. 먼저 경북 6호의 경우, 수분함량은78.02±0.16%로 경북1호의 82.61±0.07%로 다소 낮았으며,조지질 함량은 1.7배 높았다. 반면 조단백 및 조섬유 함량은경북 1호가 경북6호보다 1.6배정도 높았다. 회분 함량은 유사하였다. 절단면의 색차는 경북 6호의 명도가 60.7, 황색도가 11.01로 경북1호의 명도 65.33, 황색도가 4.85보다 약간어두운 황색을 띄었다. 총 polyphenol함량은 경북 6호가 평균 89.45 mg/g으로 경북 1호의 43.91 mg/g의 2배에 해당하였으며, 특히 ethylacetate 분획은 144.1±3.20 mg/g의 매우높은 함량을 나타내었다. 총 flavonoid 및 총당 함량은 비슷하였다. DPPH radical 소거능 평가 결과, 경북 6호는ethylacetate 분획 > methanol 추출물 > butanol 분획 > hexane 분획 >물 분획 순으로 우수하였으며, 가장 우수한 소거능을나타낸 ethylacetate 분획의 IC50는 78.32 μg/mL이었다. 환원성 평가 결과, 물분획 > methanol 추출물 > ethylacetate 분획 > butanol 분획 > hexane 분획 순으로 나타나 수용성 물질이 강력한 환원성을 나타내었다. 한편 경북 6호의 n-hexane및 ethylacetate 분획에서 B. subtilis, L .monocytogenes, S. epidermidis, S. aureus, P. vulgaris, 및S. typhimurium에 대해 우수한 항균력을 나타내었다. 본 연구 결과는 국내 재배된 D. alata L.의 기초자료로 이용될 것이다.
Abstract
Yam (Dioscorea spp.) has been used as important edible and medicinal natural resource in worldwide and D. alata L. is most popular nourishment among the yam. In this study the composition,color, antioxidation and antimicrobial activity of D. alata Gyeongbuk No. 6 (GB-6), which was established in Gyeongbuk Agricultural Research & Extension Services, Andong, Korea, was compared to those of D. batatas Gyeongbuk No. 1 (GB-1), a major domestic cultivation species. Water content of GB-6 was 78.02±0.16%, which is slightly lower than that of GB-1 (82.61±0.07%). The contents of crude protein, crude fat, crude fiber and ash of GB-6 were 0.95, 0.26, 0.85 and 0.70%, whereas those of GB-1 were 1.58, 0.15, 1.39and 0.88%, respectively. Analysis of color using colormeter showed that the GB-6 is slight dark-yellow than GB-1, and total polyphenol content of GB-6 was 2-fold higher compared than that of GB-1. Sequential organic solvent fractions from methanol extract of GB-6 showed that the ethylacetate fraction has highest total polyphenol (144.1±3.20 mg/g). Determination of antioxidation activity showed that the ethylacete fraction and water fraction has strong DPPH radical scavenging activity (IC50=78.32 μg/mL) and reducing power,respectively. In antimicrobial activity assay, the n-hexane and ethylacetate fraction showed antibacterial activity against B. subtilis, L .monocytogenes, S. epidermidis, S. aureus, P. vulgaris, and S. typhimurium. These results provide the possibility of domestic cultivated D. alata GB-6 as a healthy food.
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