마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가
Evaluation of Antimicrobial, Antithrombin, and Antioxidant Activity of Dioscorea batatas Decne
2
조회
0
다운로드
41
인용
0
참고문헌
키워드
초록
Yam (Dioscorea batatas Decne) has been recognized as healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, anti-mutagenic activity, and decrease of blood glucose and cholesterol level. In this study, the methanol extract and its organic solvent fractions were prepared from dried Dioscorea batatas Decne, and their antimicrobial, antithrombin, and antioxidant activity were evaluated, respectively. The 66.7% of methanol extract was fractioned into water residue, and most of total-polyphenol/total-flavonoids were found in ethylacetate fraction. This ethylacetate fraction at 500 μg/disc concentration showed strong antibacterial activity against gram-negative and gram-positive bacteria, except Staphylococcus aureus. Antifungal activity was not observed in methanol extract and its fractions. But, strong antithrombin activity was found in methanol extract. At 4.8 mg/mL concentration, thrombin time was 99.5 sec, which is coincides with 30% activity of aspirin, a commercial available antithrombosis agent. The ethylacetate fraction showed strong DPPH scavenging activity (IC50 of 80.5 μg/mL), compared to vitamin C (IC50 of 15.2 μg/mL). Also, the ethylacetate fraction showed strong SOD-like activity and reducing power, which are coincide with 43% of vitamin C and 82.7% of butylated hydroxytoluene activity, respectively.
Abstract
Yam (Dioscorea batatas Decne) has been recognized as healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, anti-mutagenic activity, and decrease of blood glucose and cholesterol level. In this study, the methanol extract and its organic solvent fractions were prepared from dried Dioscorea batatas Decne, and their antimicrobial, antithrombin, and antioxidant activity were evaluated, respectively. The 66.7% of methanol extract was fractioned into water residue, and most of total-polyphenol/total-flavonoids were found in ethylacetate fraction. This ethylacetate fraction at 500 μg/disc concentration showed strong antibacterial activity against gram-negative and gram-positive bacteria, except Staphylococcus aureus. Antifungal activity was not observed in methanol extract and its fractions. But, strong antithrombin activity was found in methanol extract. At 4.8 mg/mL concentration, thrombin time was 99.5 sec, which is coincides with 30% activity of aspirin, a commercial available antithrombosis agent. The ethylacetate fraction showed strong DPPH scavenging activity (IC50 of 80.5 μg/mL), compared to vitamin C (IC50 of 15.2 μg/mL). Also, the ethylacetate fraction showed strong SOD-like activity and reducing power, which are coincide with 43% of vitamin C and 82.7% of butylated hydroxytoluene activity, respectively.
참고문헌 (26)
- 안정희, "부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산", Journal of Plant Biotechnology, 32(3), pp.217~223 (2005), 한국식물생명공학회
- Han, Y. N, "Purification of mucilages from Dioscorea batatas and D. japonica and their content analysis", Kor. J. Pharmacogn, 21, pp.274~283 (1990)
- Hironaka, K, "Chemical composition of mucilage of chinese yam", Nippon Shokuhin Kogyo Gakkaishi, 37, pp.48~51 (1990)
- 김선여, "산약의 항당뇨 특성 연구(1) - 혈당 강하 효과 -", 한국식품영양학회지, 21(4), pp.425~429 (2008), 한국식품영양학회
- 김선여, "산약의 항당뇨 특성 연구(2) - NGF 유도 신경병증예방 -", 한국식품영양학회지, 21(4), pp.430~435 (2008), 한국식품영양학회
- Kim M. W, "Effects of H2O-fraction of Dioscorea japonica Thunb and selenium on lipid peroxidation in streptozotocin-induced diabetic rats", Kor. J. Soc. Food Cookery Sci., 17, pp.344~352 (2001)
- 김은영, "항산화활성을 나타내는 약용식물 소재 탐색", 한국식품과학회지, 36(2), pp.333~338 (2004), 한국식품과학회
- 김정옥, "상황버섯 열수 및 에탄올 추출물의 항산화 효과 및 생리활성", 한국식품영양과학회지, 37(6), pp.684~690 (2008), 한국식품영양과학회
- 손호용, "재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성", 생명과학회지, 16(4), pp.647~652 (2006), 한국생명과학회
- Kwon, C. S, "Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents", Biosci. Biotechnol. Biochem, 67, pp.1451~1456 (2003)
- Kwon, C. S., "Effects of yam on lowering cholesterol level and its mechanism", Kor. J. Food Nutr., 32, pp.637~643 (1999)
- Kwon, E. G, "Effects of mucilage from yam (Dioscorea batatas DECNE) on blood glucose and lipid composition in alloxan-induced diabetic mice", Kor. J. Food Sci. Technol, 33, pp.795~801 (2001)
- Lee I.-S, "Inhibitory effects of yam (Dioscorea batatas DECNE) extracts on the mutagenicity", Kor. J. Soc. Food Sci, 11, pp.351~355 (1995)
- Lee, B. Y, "Quality properties of korean yam by various drying methods", Kor. J. Food Sci. Technol, 30, pp.877~882 (1998)
- Marklund, S, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur. J. Biochem, 47, pp.469~474 (1974)
- Na, M, "Effect of the rhizomes of Astilbe chinensis on UVB-induced inflammatory response", Phytother. Res., 18, pp.1000~1004 (2004)
- 박찬성, "한약재 추출물의 항산화작용 및 아질산염 소거작용", 한국식품저장유통학회지, 12(6), pp.631~636 (2005), 한국식품저장유통학회
- Park, C. S, "Functional properties of medicinal plant extracts", Kor. J. Food. Cookery Sci, 23, pp.720~727 (2006)
- 배경화, "안동소주 발효액으로부터 분리한 젖산 세균의 동정 및 발효 특성", 한국미생물·생명공학회지, 35(4), pp.310~315 (2007), 한국미생물·생명공학회
- 류희영, "클로버 오일을 이용한 생마 저온부패균의 제어 및 기능성 생마 신선편이의 제조", 한국미생물·생명공학회지, 35(1), pp.66~72 (2007), 한국미생물·생명공학회
- 류희영, "생마 저온부패 원인세균의 분리 및 부패균의 특성", 한국미생물·생명공학회지, 34(2), pp.109~114 (2006), 한국미생물·생명공학회
- Shin, K. O, "Lactic acid fermentation of chinese yam (Dioscorea batatas Decne) flour and its pharmacological effect on gastrointestinal function in rat model. Biotechnol", Bioprocess Eng, 11, pp.240~244 (2006)
- Shin, S. R, "Changes on the components of yam snack by processing methods", Kor. J. Food Preservation, 11, pp.516~521 (2004)
- 손호용, "호이초(Saxifraga stolonifera) 지상부의 항균, 항혈전 및 항산화 활성 평가", 한국미생물·생명공학회지, 36(3), pp.195~200 (2008), 한국미생물·생명공학회
- 손호용, "메밀 종자의 항트롬빈 활성과 혈전증 예방효과", 한국식품영양과학회지, 35(2), pp.132~138 (2006), 한국식품영양과학회
- Sohn, H. Y, "Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai", Phytomedicine, 11, pp.666~672 (2004)