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Cellulophaga lytica PKA 1005의 Cellulose 분해 조효소 생산 최적조건과 조효소의 특성

Optimization and Characterization of Conditions for Cellulose-degrading Crude Enzymes Produced by Cellulophaga lytica PKA 1005

김보람|안동현|김민지|임성미|강보경|박홍민|김꽃봉우리|박원민|박시우
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2014.03|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제42권 제1호|pp. 18-24|DOI 10.4014/kjmb.1311.11004

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참고문헌

키워드

Cellulophaga lyticacellulose-degrading crude enzyme
주제분류생물학

초록

This study was conducted to investigate optimum conditions for the production of cellulose-degrading crude enzymes by anisolated marine bacterium. A marine microorganism producing an extracellular cellulose-degrading enzyme was isolated fromthe red seaweed, Grateloupia elliptica Holmes. The isolated bacterium was identified as Cellulophaga lytica by 16S ribosomalRNA gene sequence analysis and physiological profiling and designated as Cellulophaga lytica PKA 1005. The optimum conditionsfor the growth of Cellulophaga lytica PKA 1005 were pH 7, 2% NaCl, and 30oC with 36 h incubation time. To obtain thecrude enzyme, the culture medium of the strain was centrifuged for 30 min at 12,000 × g and 4oC, and the supernatant wasused as crude enzyme. The optimum conditions for the production of the cellulose-degrading crude enzyme were pH 8, 35oC,8% carboxyl methyl cellulose, and 60 h reaction time.

Abstract

This study was conducted to investigate optimum conditions for the production of cellulose-degrading crude enzymes by anisolated marine bacterium. A marine microorganism producing an extracellular cellulose-degrading enzyme was isolated fromthe red seaweed, Grateloupia elliptica Holmes. The isolated bacterium was identified as Cellulophaga lytica by 16S ribosomalRNA gene sequence analysis and physiological profiling and designated as Cellulophaga lytica PKA 1005. The optimum conditionsfor the growth of Cellulophaga lytica PKA 1005 were pH 7, 2% NaCl, and 30oC with 36 h incubation time. To obtain thecrude enzyme, the culture medium of the strain was centrifuged for 30 min at 12,000 × g and 4oC, and the supernatant wasused as crude enzyme. The optimum conditions for the production of the cellulose-degrading crude enzyme were pH 8, 35oC,8% carboxyl methyl cellulose, and 60 h reaction time.

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