Isolation and Culture Properties of a Thermophilic Agarase-Producing Strain, Microbulbifer sp. SD-1
3
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
An agar-degrading enzyme-producing strain was isolated from seawater. The isolate was identified as Microbulbifer sp. SD-1 by 16S rRNA sequencing analysis. The optimal pH and temperature for growth were 6.0 and 30°C, respectively, and growth was possible at pH 9.0 and 60°C. The isolate required 5% NaCl for optimal growth and showed 45% growth activity without NaCl. Agar concentrations of 0-0.4% in the medium did not affect growth. Thin-layer chromatography analysis revealed that this strain could degrade agar into a monosaccharide and oligosaccharide, which may have industrial applications.
Abstract
An agar-degrading enzyme-producing strain was isolated from seawater. The isolate was identified as Microbulbifer sp. SD-1 by 16S rRNA sequencing analysis. The optimal pH and temperature for growth were 6.0 and 30°C, respectively, and growth was possible at pH 9.0 and 60°C. The isolate required 5% NaCl for optimal growth and showed 45% growth activity without NaCl. Agar concentrations of 0-0.4% in the medium did not affect growth. Thin-layer chromatography analysis revealed that this strain could degrade agar into a monosaccharide and oligosaccharide, which may have industrial applications.
참고문헌 (13)
- Berns KI, "Isolation of high molecular weight DNA from Hemophilus influenzae", J Mol Biol, 11, pp.476~490 (1965)
- Duckworth M, "Thin-layer chromatographic analysis of enzymic hydrolysates of agar", J Chromatogr, 49, pp.482~487 (1970)
- Dunbar J, "Assessment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis", Appl Environ Mirobiol, 66, pp.2943~2950 (2000)
- Ensor LA, "Expression of multiple complex polysaccharide-degrading enzyme systems by marine bacterium strain 2-40", J Ind Microbiol Biotechnol, 23, pp.123~126 (1999)
- Fu XT, "Agarase:review of major sources,categories,purification method,enzyme characteristics sand applications", Mar Drugs, 8, pp.200~218 (2010)
- González JM, "Microbulbifer hydrolyticus gen.nov.,sp.nov.,and Marinobacterium georgiense gen.nov.,sp.nov.,two marine bacteria from a lignin-rich pulp mill waste enrichment community", Int J Syst Bacteriol, 47, pp.369~376 (1997)
- Jonnadula R, "Characterization of Microbulbifer strain CMC-5,a new biochemical variant of Microbulbifer elongatus type strain DSM6810T isolated from decomposing seaweeds", Curr Microbiol, 59, pp.600~607 (2009)
- 이상현, "한천분해효소를 생산하는 해양세균 Thalassomonas sp. SL-5의 분리 및 특성", 생명과학회지, 17(2), pp.70~75 (2007), 한국생명과학회
- 이재화, "한천분해효소를 생산하는 Agarivorans sp. JA-1의 배양조건 및 Fed-batch 배양", KSBB Journal, 21(5), pp.389~393 (2006), 한국생물공학회
- Ohta Y, "Purification and characterization of a novel alpha-agarase from a Thalassomonas sp", Curr Microiol, 50, pp.212~216 (2005)
- 이영준, "Isolation of a Novel Freshwater Agarolytic Cellvibrio sp. KY-YJ-3 and Characterization of Its Extracellular β-Agarase", Journal of Microbiology and Biotechnology, 20(10), pp.1378~1385 (2010), 한국미생물·생명공학회
- Suzuki H, "Purification and characterization of an extracellular α-neoagarooligosaccharide hydrolase from Bacillus sp.MK03", J Biosci Bioeng, 93, pp.456~463 (2002)
- Yoon JH, "Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea", Int J Syst Evol Microbiol, 54, pp.1111~1116 (2004)