된장에서 분리된 Bacillus licheniformis의 β-galactosidase 생산성과 효소특성
Production and Characterization of β-galactosidase from Bacillus licheniformis Isolated from Doenjang
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초록
A bacterial strain was isolated from homemade doenjang (Korean fermented soybean paste) as a producer of the extracellular β-galactosidase, capable of hydrolyzing lactose to liberate galactose and glucose residues. The isolate YB-1414 has been identified as Bacillus licheniformis on the basis of its 16S rDNA sequence, morphology and biochemical properties. The production of β-galactosidase by B. licheniformis YB-1414 reached maximum levels of 6.2 U/ml in culture medium containing wheat bran (1%) and yeast extract (2.5%) as carbon and nitrogen sources, respectively. Particularly, the insoluble fraction was more effective for β-galactosidase production than the soluble extract of wheat bran. The enzyme exhibited maximum activity for hydrolysis of para-nitrophenyl-β-D-galactopyranoside (pNP-βGal) under reaction conditions of pH 6.0 and 55-60oC. Its hydrolyzing activity for pNP-βGal was drastically decreased by the addition of low concentrations of galactose, but only slightly decreased by glucose, with 85% of maximal activity in the presence of 400 mM glucose.
Abstract
A bacterial strain was isolated from homemade doenjang (Korean fermented soybean paste) as a producer of the extracellular β-galactosidase, capable of hydrolyzing lactose to liberate galactose and glucose residues. The isolate YB-1414 has been identified as Bacillus licheniformis on the basis of its 16S rDNA sequence, morphology and biochemical properties. The production of β-galactosidase by B. licheniformis YB-1414 reached maximum levels of 6.2 U/ml in culture medium containing wheat bran (1%) and yeast extract (2.5%) as carbon and nitrogen sources, respectively. Particularly, the insoluble fraction was more effective for β-galactosidase production than the soluble extract of wheat bran. The enzyme exhibited maximum activity for hydrolysis of para-nitrophenyl-β-D-galactopyranoside (pNP-βGal) under reaction conditions of pH 6.0 and 55-60oC. Its hydrolyzing activity for pNP-βGal was drastically decreased by the addition of low concentrations of galactose, but only slightly decreased by glucose, with 85% of maximal activity in the presence of 400 mM glucose.
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