α-Galactosidase를 생산하는 고온성 Bacillus coagulans KM-1 균주의 생화학적 특성
Biochemical Characterization of α-Galactosidase-Producing Thermophilic Bacillus coagulans KM-1
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
A bacterium producing α-galactosidase (α-d-galactoside galactohydrolase, EC 3.2.1.22) was isolated. The isolate,KM-1 was identified as Bacillus coagulans based on its 16S rRNA sequence, morphology, and biochemical properties. α-Galactosidase activity was detected the culture supernatant of B. coagulans KM-1. The bacterium showed the maximumactivity for hydrolyzing para-nitrophenyl-α-d-galactopyranoside (pNP-αGal) at pH 6.0 and 50°C. It hydrolyzedoligomeric substrates such as melibiose, raffinose, and stachyose liberating a galactose residue, indicating that the B. coagulans KM-1 α-galactosidase hydrolyzed α-1,6 linkage. The results suggest that the decreased stachyose and raffinosecontents in fermented soybean meal are due to the α-galactosidase activity.
Abstract
A bacterium producing α-galactosidase (α-d-galactoside galactohydrolase, EC 3.2.1.22) was isolated. The isolate,KM-1 was identified as Bacillus coagulans based on its 16S rRNA sequence, morphology, and biochemical properties. α-Galactosidase activity was detected the culture supernatant of B. coagulans KM-1. The bacterium showed the maximumactivity for hydrolyzing para-nitrophenyl-α-d-galactopyranoside (pNP-αGal) at pH 6.0 and 50°C. It hydrolyzedoligomeric substrates such as melibiose, raffinose, and stachyose liberating a galactose residue, indicating that the B. coagulans KM-1 α-galactosidase hydrolyzed α-1,6 linkage. The results suggest that the decreased stachyose and raffinosecontents in fermented soybean meal are due to the α-galactosidase activity.
참고문헌 (20)
- Coombs J, "Characterization of two new glicosyl hydrolases from the lactic acid bacterium Carnobacterium piscicola strain BA", Appl Environ Microbiol, 67, pp.5094~5099 (2001)
- Choi C, "Characteristics and action pattern of α-galactosidase from Scopulariopsis brevicaulis in Korean traditional Meju", ACB, 41, pp.489~495 (1998)
- Dey PM, "Biochemistry of the multiple forms of glycosidases in plants", Adv Enzymol Relat Areas Mol Biol, 56, pp.141~249 (1984)
- Halstead JR, "α-galactosidase a from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis", FEMS Microbiol Lett, 192, pp.197~203 (2000)
- Irish GG, "Removal of the α-galactosidase of sucrose from soybean meal using either ethanol extraction or exogenous alpha-galactosidase and broiler performance", Poult Sci, 74, pp.1484~1494 (1995)
- Jang MS, "Effect of extruded pellets containing fermented soybean meal as a partial substitute for fish meal on growth performance and muscle quality of olive flounder (Paralichthys olivaceus).", J Agric Life Sci, 47, pp.203~215 (2013)
- Jindou S, "α-galactosidase Agar27A, an enzymatic component of the Clostridium josui cellulosome", J Bacteriol, 184, pp.600~604 (2002)
- 김강웅, "치어기 넙치(Paralichthys olivaceus) 사료내 어분 대체원으로서발효 대두박 이용성", 한국수산과학회지, 46(6), pp.769~776 (2013), 한국수산과학회
- KIM, JEONG-DONG, "Purification and Characterization of Extracellular β-Glucosidase fromSinorhizobium kostiense AFK-13 and Its Algal Lytic Effect on Anabaena flos-aquae", Journal of Microbiology and Biotechnology, 17(5), pp.745~752 (2007), 한국미생물·생명공학회
- Kim JS, "The Changes of α-galactosidase activities and stachyose and raffinose contents during fermentation of soybeans", Korea J Soc Food Sci, 14, pp.509~512 (1998)
- Li DF, "Transient hypersensitivity to soybean meal in the early-weaned pig", J Anim Sci, 68, pp.1790~1799 (1991)
- Min BJ, "Influence of dietary carbohydrase on egg quality and nutrient digestibility in laying hens", Korea J Poult Sci, 29, pp.19~23 (2002)
- Rackis JJ, "Flatulence caused by soya and its control through processing", J Am Oil Chem Soc, 58, pp.503~510 (1981)
- Reddy NR, "Flatulence in rats following ingestion of cooked and germinated black and a fermented product of black gram and rice blend", J Food Sci, 45, pp.1161~ (1980)
- Silvestroni A, "Characterization of the mel locus for α-galactosidase in Lactobacillus plantarum", Appl Environ Microbiol, 68, pp.5464~5471 (2002)
- Scalabrini, Rossi P, "Characterization of Bifidobacterium strains for use in soymilk fermentation", Int J food microbiol, 39, pp.213~219 (1998)
- Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Steggerda FR, "Effects of various soybean products on flatulence in the adult man", Proc Soc Exp Biol Med, 121, pp.1235~ (1968)
- Sanada CTN, "Utilization of soybean vinasse for α-galactosidase production", Food Res Int, 42, pp.476~483 (2009)
- 유종상, "환경친화적인 사료의 급여가 육성돈의 성장 능력, 영양소 소화율, 분 배설량, 분뇨내 질소배설량 및 악취 가스에 미치는 영향", 한국동물자원과학회지, 49(4), pp.491~500 (2007), 한국동물자원과학회