Long-term Photosynthetic Chlorella Cultivation in Recycled Medium for Sustainable Lipid Production
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Effective recycling of spent medium is crucial for scaling up photosynthetic microalgal cultivation and achieving sustainable biofuel production. In this study, we investigated the long-term cultivation of Chlorella sp. KR-1 in a 1-L bubble-column photobioreactor over a 49-day period using recycled culture medium. Neutral lipid content, predominantly triacylglycerols—a promising feedstock for next-generation biofuels—was quantified as fatty acid methyl esters (FAMEs). Control cultures were grown in fresh N8 medium, while the experimental groups were grown in recycled medium supplemented with full N8 nutrients. Photosynthetic cell growth remained stable during the first three cycles, with final biomass concentrations of 3.07 ± 0.16 and 3.08 ± 0.17 g/L in the second and third cycles, respectively, slightly exceeding the control (2.96 ± 0.09 g/L). In later cycles (four to seven), biomass production gradually declined, stabilizing between 2.57 and 2.69 g/L. FAME productivity peaked at 135 mg FAMEs/L/day during the second and third cycles, representing a 12% increase over that of the control, before declining to 88 mg FAMEs/L/day by the seventh cycle. These findings suggest that recycling the culture medium, with appropriate management of inhibitory byproducts, can sustain microalgal cultivation with minimal reductions in biomass and lipid productivity.
Abstract
Effective recycling of spent medium is crucial for scaling up photosynthetic microalgal cultivation and achieving sustainable biofuel production. In this study, we investigated the long-term cultivation of Chlorella sp. KR-1 in a 1-L bubble-column photobioreactor over a 49-day period using recycled culture medium. Neutral lipid content, predominantly triacylglycerols—a promising feedstock for next-generation biofuels—was quantified as fatty acid methyl esters (FAMEs). Control cultures were grown in fresh N8 medium, while the experimental groups were grown in recycled medium supplemented with full N8 nutrients. Photosynthetic cell growth remained stable during the first three cycles, with final biomass concentrations of 3.07 ± 0.16 and 3.08 ± 0.17 g/L in the second and third cycles, respectively, slightly exceeding the control (2.96 ± 0.09 g/L). In later cycles (four to seven), biomass production gradually declined, stabilizing between 2.57 and 2.69 g/L. FAME productivity peaked at 135 mg FAMEs/L/day during the second and third cycles, representing a 12% increase over that of the control, before declining to 88 mg FAMEs/L/day by the seventh cycle. These findings suggest that recycling the culture medium, with appropriate management of inhibitory byproducts, can sustain microalgal cultivation with minimal reductions in biomass and lipid productivity.
참고문헌 (29)
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